Alternaria panax Whetz, Cylindrocarpon destructans, Botrytis cinerea Pers, Sclerotionia schinseng, Phytophora cactorum Schroet, and Escherichia coli were provided by the Plant Pathology Laboratory of Jilin Agricultural University. PCR Master Mix kit (ABI USA); Proteinase K; EDTA, Tris-HCl, NaCl, magnetic nanoparticles, isopropanol, 70% ethanol, and BSA-Biotin conjugate (Sangon Biotech Co., Ltd, Shanghai, China); 30 nm colloidal gold particles, gold absorbent pad (GL0194), and nitrocellulose filter membrane (Millipore 135) (Jiyi Biotech Co., Ltd, Shanghai, China); mouse anti-Fam antibody (Gei Man Biotech Co., Ltd, Guangzhou, China); streptavidin (Ruijin Biotech Co., Ltd, Shanghai, China), biosensor reader instrument (JY1501GS, Jie Yi Biotech Co., Ltd, Shanghai, China).
Millipore 135
Manufactured by Merck Group
Sourced in China, United States
The Millipore 135 is a laboratory filtration device. It is used for the filtration of liquids, such as water or other solvents, through a membrane filter. The device provides a controlled and consistent filtration process.
Automatically generated - may contain errors
Lab products found in correlation
Citrinin, by Merck Group (1 mentions)
Hypoxanthine aminopterin, by Merck Group (1 mentions)
Fumonisin b1, by Merck Group (1 mentions)
Millipore 180, by Merck Group (1 mentions)
Hypoxanthine thymidine medium, by Merck Group (1 mentions)
Polyethylene glycol peg 1450, by Merck Group (1 mentions)
Ochratoxin a, by Merck Group (1 mentions)
Aflatoxin b1, by Merck Group (1 mentions)
3 protocols using millipore 135
1
Rapid Pathogen Detection Protocol
2
Rapid Immunoassay Strip Fabrication
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The nitrocellulose membrane (Millipore 135) was cut to 25 mm wide and 100 mm long and then pasted to the middle of the PVC substrate. Goat anti-mouse IgG (1 mg/mL) or goat anti-rabbit IgG (1 mg/mL) was dispensed onto the control area to prepare the control line, and RAC-BSA (1 mg/mL) or SAL-BSA (0.05 mg/mL) was dispensed onto the detection area to prepare the test line. After 3 hours of drying at 37 °C in an electric blast drying oven, the samples were removed and placed into a sealed plastic bucket.
3
Mycotoxin Detection Assay Development
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Citrinin (CIT), Aflatoxin B1 (AFB1), Fumonisin B1 (FB1), Patulin (PAT), Ochratoxin A (OTA), polyethylene glycol (PEG) 1450, hypoxanthine aminopterin and thymidine (HAT) medium, hypoxanthine-thymidine (HT) medium, complete and incomplete Freund adjuvant were purchased from Sigma (USA). The mouse McAb isotyping kit was purchased from Hycult Biotechnology (Netherlands). The SP2/0 myeloma cell line was stored in our lab. The Ridascreen Fast CIT ELISA kit was purchased from the R-Biopharm group (Germany). Goat anti-mouse IgG (H+L) was purchased from BioWorld (USA). Protein-G Sepharose Fast Flow Columns were purchased from GE Healthcare (USA). Chloroauricacid (HAuCl4) and trisodium citrate were obtained from Shanghai Chemical Reagents (Shanghai, China). The nitrocellulose membranes (Millipore 135, Millipore 180) were purchased from Millipore (USA). Othertypes of nitrocellulose membranes (Sartorius CN 140, PALL Vivid 170), conjugation pads (GL0194, Ahlstrom 8964, Ahlstrom 6613), sample pads (GL-b01, GL-b02) and absorbent pads were purchased from Jieyi Bio. Co. (China).
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