Total RNA was extracted by using TRIzol reagent (Invitrogen). 1 μg RNA was reverse transcribed to cDNA using Reverse Transcription Master Kit (Takara) according to the manufacturer’s instructions. For quantitative PCR analysis, aliquots of cDNA were amplified using TB Green Premix Ex Taq II (Takara) and ViiATM7/QuantStudio 7 Flex Real Time PCR System (Applied Biosystems). GAPDH was used as an internal control. All reactions were performed in triplicate. The sequences of primers used are listed in the key resources table .
Viiatm7 quantstudio 7 flex real time pcr system
Manufactured by Thermo Fisher Scientific
Sourced in United States
The ViiA™7/QuantStudio 7 Flex Real-Time PCR System is a high-performance real-time PCR instrument designed for accurate and reliable genetic analysis. It features a six-channel optical detection system, advanced temperature control, and a flexible configuration to accommodate a variety of experimental needs.
Automatically generated - may contain errors
Lab products found in correlation
Reverse transcription master kit, by Takara Bio (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Tb green premix ex taq 2, by Takara Bio (1 mentions)
First strand cdna synthesis kit, by Takara Bio (1 mentions)
Trizol reagent, by Takara Bio (1 mentions)
Sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions)
2 protocols using viiatm7 quantstudio 7 flex real time pcr system
1
RNA Extraction and qPCR Analysis
2
Quantitative Real-Time PCR Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNAs from tissue samples or cell lines were extracted by the TRIzol reagent (Takara, Japan). Complementary DNA strands were reverse-transcribed by the first-strand cDNA synthesis kit (Takara, Japan). SYBR Green PCR Master Mix (Applied Biosystems, USA) were used for qRT-PCR analysis on the ViiATM7/QuantStudio 7 Flex Real Time PCR System (Applied Biosystems, USA). GAPDH was used as internal control. Relative gene expression was calculated by the 2−ΔΔCq method. The primers used for qRT-PCR analysis were listed in Supplementary Table 3 .
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