Anti phospho gcn2 t899

Manufactured by Abcam

Sourced in United Kingdom

Anti-phospho-GCN2 (T899) is a primary antibody that specifically detects the phosphorylated form of the GCN2 protein at threonine 899. GCN2 is a serine/threonine protein kinase that plays a role in the cellular response to amino acid starvation.

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2 protocols using anti phospho gcn2 t899

Antibody Immunoblotting and Immunofluorescence

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Primary antibodies used were anti-viperin (MaP.VIP) (Wang et al., 2007 (link)), anti-GRP94 (Enzo), anti-GAPDH (Proteintech), anti-phospho-4EBP1 (Cell Signaling), anti-4EBP1 (Cell Signaling), anti-PRS6 (Cell Signaling), anti-phospho-RPS6 (Cell Signaling; 2217), anti-phospho-eIF2α (S51) (Cell Signaling), anti-eIF2α (Cell Signaling), anti-Flaviviral E protein (Millipore), anti-GCN2 (Cell Signaling), anti-phospho-GCN2 (T899) (Abcam), anti-PERK (Cell Signaling), anti-HRI (Proteintech), anti-PKR (Cell Signaling), anti-ZAK (Bethyl). All secondary antibodies used for immunofluorescence imaging (goat antimouse IgG coupled to Alexa Fluor 488, Alexa Fluor 647, goat anti-rat IgG coupled to Alexa Fluor 546, Alexa Fluor 594 and goat anti-rabbit IgG coupled to Alexa Fluor 647) were purchased from Invitrogen, and those for immunoblotting (goat anti-mouse IgG and anti-rabbit and anti-rat IgG coupled to horseradish peroxidase and alkaline phosphatase) were purchased from Jackson ImmunoResearch.

Hsu J.C., Laurent-Rolle M., Pawlak J.B., Xia H., Kunte A., Hee J.S., Lim J., Harris L.D., Wood J.M., Evans G.B., Shi P.Y., Grove T.L., Almo S.C, & Cresswell P. (2022). Viperin triggers ribosome collision-dependent translation inhibition to restrict viral replication. Molecular cell, 82(9), 1631-1642.e6.

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Immunoblotting of Cell Cycle and Stress Regulators

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Immunoblotting was carried out as previously described [48 (link)]. Commercially available antibodies used were as follws: anti-p21 (sc-6246; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-p27 (610241; BD Biosciences, Franklin Lakes, NJ, USA), anti-cyclin A (611268; BD Biosciences), anti-cyclin D1 (556470; BD Biosciences), anti-ATF4 (11815; Cell Signaling Technology, Beverly, MA, USA), anti-PERK (5683; Cell Signaling Technology), anti-GCN2 (3302; Cell Signaling Technology), anti-TRB3 (ab75846; Abcam, Cambridge, UK), anti-phospho-GCN2 (T899) (ab75836; Abcam), anti-phospho-PKR (T451) (07-886; Sigma) anti-β-actin (A5441; Sigma), and anti-PKR (MAB1980; R&D Systems, Minneapolis, MN, USA).

Nishikawa S., Itoh Y., Tokugawa M., Inoue Y., Nakashima K.I., Hori Y., Miyajima C., Yoshida K., Morishita D., Ohoka N., Inoue M., Mizukami H., Makino T, & Hayashi H. (2019). Kurarinone from Sophora Flavescens Roots Triggers ATF4 Activation and Cytostatic Effects Through PERK Phosphorylation. Molecules, 24(17), 3110.

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