Jurkat cells were harvested 5 days after lentiviral infection after transduction and lysed in RIPA lysis buffer (PC101, Epizyme Biotech). Then, the protein samples were mixed with 1x SDS (LT101S; Epizyme Biotech), boiled for 10 minutes, and then subjected to PAGE gel electrophoresis. The primary antibody used in the experiment includes DEK (E4S5J; Cell Signaling Technology), GAPDH (D16H11; Cell Signaling Technology), TP53 (DO-7; Cell Signaling Technology), c-Myc (ab32072; Abcam), CDK4 (A11136; ABclonal), CDK6 (13331; Cell Signaling Technology), CDKN1A (A1483; ABclonal), CDKN2A (ab151303; Abcam), caspase-3 (9662; Cell Signaling Technology), BCL2L1 (A19703; ABclonal) at 4°C, and HRP-conjugated secondary antibody (anti-rabbit,7074S, anti-mouse; 7076S,Cell Signaling Technology) at room temperature for 2 h. The target protein was detected by using Omni-ECL™-enhanced chemiluminescent liquid (SQ101; Epizyme Biotech) and quantified using ImageQuant LAS 4000 mini (GE).
Cdkn2a
Manufactured by Abcam
CDKN2A is a gene that encodes for two distinct tumor suppressor proteins, p16INK4a and p14ARF, which play important roles in cell cycle regulation and tumor suppression. The p16INK4a protein inhibits the activity of cyclin-dependent kinases CDK4 and CDK6, thereby preventing the phosphorylation of the retinoblastoma protein (Rb) and blocking cell cycle progression. The p14ARF protein, on the other hand, stabilizes the p53 tumor suppressor protein by inhibiting its degradation. This gene is frequently altered or inactivated in a wide variety of cancers, making it an important biomarker and target for cancer research and diagnostics.
Automatically generated - may contain errors
Lab products found in correlation
Imagequant las 4000 mini, by GE Healthcare (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Gapdh d16h11, by Cell Signaling Technology (1 mentions)
C myc, by Abcam (1 mentions)
Myogenin, by Abcam (1 mentions)
Neuronal nuclei (neun), by Merck Group (1 mentions)
H3k27me3, by Cell Signaling Technology (1 mentions)
Tissue studio, by Definiens (1 mentions)
Runx2, by Abcam (1 mentions)
Tnf α, by Abcam (1 mentions)
Il 1b, by Abcam (1 mentions)
Hrp conjugated species specific secondary antibody, by Beyotime (1 mentions)
Fibroblast growth factor 2 (fgf2), by Abcam (1 mentions)
Arg 1, by Abcam (1 mentions)
β actin, by Proteintech (1 mentions)
3 protocols using cdkn2a
1
Western Blot Analysis of Cell Signaling
2
Immunohistochemical analysis of mouse brains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse brains were processed, immunostained, and quantified as previously described [67 (link)–69 (link)]. In brief, mice were placed under isoflurane anesthesia and decapitated. Harvested brains were fixed by immersion in 4% formaldehyde for 24 h and then transferred to a graded ethanol series and embedded in paraffin and sectioned along the sagittal midline. Samples were stained and imaged using an Aperio Scanscope and quantified via automated cell counting using Tissue Studio (Definiens).
Primary antibodies used were: H3K27me3 diluted 1:200 (Cell Signaling, #9733), pRB diluted 1:3000 (Cell Signaling, #8516), cC3 diluted 1:400 (Biocare Medical, #CP229C), NeuN diluted 1:10,000 (Millipore, MAB377), Myogenin (MYOG) diluted 1:500 (Abcam, ab124800), SMYD1 diluted 1:100 (ThermoFisher, PA5-84544), and CDKN2A diluted 1:500 (Abcam, ab241543). Stained images were counterstained with DAPI.
Primary antibodies used were: H3K27me3 diluted 1:200 (Cell Signaling, #9733), pRB diluted 1:3000 (Cell Signaling, #8516), cC3 diluted 1:400 (Biocare Medical, #CP229C), NeuN diluted 1:10,000 (Millipore, MAB377), Myogenin (MYOG) diluted 1:500 (Abcam, ab124800), SMYD1 diluted 1:100 (ThermoFisher, PA5-84544), and CDKN2A diluted 1:500 (Abcam, ab241543). Stained images were counterstained with DAPI.
3
Protein Expression Analysis in Graft Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein samples were extracted from graft tissues. Equal masses of protein samples were separated by SDS-PAGE, and transferred to PVDF membranes that were blocked and reacted with primary antibodies according to the manufacturer's recommendations. The antibodies used include: CDKN2A (1:600, Abcam), TNFAIP6 (1:800, Abcam), ESR2 (1:400, Abcam), UCN (1:400, LifeSpan BioSciences), IL1B (1:1000, Abcam), VIP (1:1000, Abcam), NFKBIA (1:1000, Abcam), GAL (1:600, Santa), CSF1 (1:400, Santa), JUN (1:1000, Abcam), ARG1 (1:800, Abcam), FGF2 (1:1000, Abcam), ATF3 (1:1000, Abcam), ZFP36 (1:400, Millipore), CCL2 (1:1000, Millipore), GFI1 (1:400, Abcam), CCK (1:1000, Santa), RUNX2 (1:600, Abcam), TNFα (1:600, Abcam), and β-actin (1:4,000, Proteintech). The specific binding of primary antibody was detected by HRP-conjugated species-specific secondary antibody (Beyotime, Shanghai, China) and enhanced chemiluminescence (ECL) assay.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!