HepG2 and Hep3B cells were seeded onto coverslips, incubated 24 h and then fixed with 4% paraformaldehyde. Cells were incubated with primary antibodies (P300, 1:100, abcam, Cambridge, UK; Elk1, 1:100, abcam, Cambridge, UK) for 1 h at room temperature. CoraLite 488-conjugated secondary antibodies and CoraLite 594-conjugated secondary antibodies (Proteintech Group, Rosemont, IL, USA) were added for 30 min at room temperature at a dilution of 1:200 in PBS. Cells were then mounted with mounting medium (Vector Laboratories, Burlingame, CA, USA). Images were captured with a Carl Zeiss LSM 710 laser scanning confocal microscope (Carl Zeiss, Oberkochen, Germany).
Coralite 594 conjugated secondary antibodies
Manufactured by Proteintech
Sourced in United States
CoraLite 594-conjugated secondary antibodies are fluorescently labeled antibodies designed for detection and visualization in immunoassays. These antibodies are conjugated to the CoraLite 594 fluorescent dye, which has an excitation/emission spectrum suitable for red fluorescence detection.
Automatically generated - may contain errors
Lab products found in correlation
Mounting medium, by Vector Laboratories (1 mentions)
Coralite 488 conjugated secondary antibodies, by Proteintech (1 mentions)
Lsm 710 confocal laser scanning microscope, by Zeiss (1 mentions)
Ultrapure water machine, by Merck Group (1 mentions)
Caspase 3 19677 1 ap, by Proteintech (1 mentions)
Survivin sirna, by GenePharma (1 mentions)
β actin 20536 1 ap, by Proteintech (1 mentions)
Triton x 100, by Maclin Biochemical Technology (1 mentions)
Huvec cells, by American Type Culture Collection (1 mentions)
Horseradish peroxidase conjugated, by Proteintech (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Agarose, by Biowest (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
3 protocols using coralite 594 conjugated secondary antibodies
1
Immunofluorescence Analysis of HepG2 and Hep3B Cells
2
Survivin siRNA Transfection in Glioma Cells
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All oligonucleotides were synthesized and purified by Sangon Biotech Company (Shanghai, China). Survivin siRNA (5ʹ-AUUCACCAAGGGUUAAUUCdTdT-3ʹ) was synthesized by GenePharma Company (Shanghai, China). Deionized water used in all aqueous solutions was produced using a Millipore Ultrapure water machine (Massachusetts, USA). GelRed DNA gel stain solution was purchased from Biosharp (Beijing, China). Tris, magnesium chloride (MgCl2), and Triton X-100 were obtained from Macklin (Shanghai, China). Agarose was purchased from Biowest (Barcelona, Spain). U87, U251, and HUVEC cells were purchased from ATCC. Fetal bovine serum (FBS) and high-glucose Dulbecco’s modified Eagle’s medium (DMEM/high glucose) were bought from Gibco (NY, USA). A cell counting kit (CCK-8) was purchased from Bimake (Texas, USA). 4′6-Diamidino-2-phenylindole (DAPI) were obtained from Solarbio (Beijing, China). Primary antibodies β-actin (20536-1-AP), NCL (10556-1-AP), and Caspase-3 (19677-1-AP) were obtained from Proteintech (IL, USA). Survivin (YT4472) was purchased from Immunoway (TX, USA), and horseradish peroxidase-conjugated or CoraLite594-conjugated secondary antibodies were purchased from Proteintech (IL, USA).
3
Immunofluorescence Staining of β-Catenin
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IF analysis was performed as described previously (19 (link)). Briefly, cells were seeded onto chamber slides, then fixed with 4% PFA for 10 min, permeabilized for 15 min with 0.1% Triton X-100, and blocked with 5% bovine serum albumin (BSA) for 1 h. Subsequently, cells were incubated with anti-β-catenin antibody (1:100) at room temperature for another 1 h. Then the slides were washed with PBS, followed by incubation with Coralite 594-conjugated secondary antibodies (1:500, ProteinTech, USA) for 1 h. Finally, cells were stained with 4′, 6-diamidino-2-phenylindole (DAPI) (Beyotime, China) for 3 min. Images were captured with the confocal laser microscope.
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