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Percp cy5.5 cd25

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PerCP-Cy5.5-CD25 is a fluorescently labeled antibody used for the detection and analysis of CD25-expressing cells in flow cytometry applications. It consists of the PerCP-Cy5.5 fluorescent dye conjugated to an antibody specific for the CD25 cell surface marker.

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Lab products found in correlation

Foxp3 staining buffer kit, by Thermo Fisher Scientific (1 mentions) Cd62l pe cy7, by BioLegend (1 mentions) Cd25 fitc, by BD (1 mentions) Pecf594 cd4, by BD (1 mentions) Cd8α pe cf594, by BD (1 mentions) Percp efluor 710 tnf α, by Thermo Fisher Scientific (1 mentions) Cd45 fitc, by BD (1 mentions) Cd45 alexa fluor 700, by BD (1 mentions) Allophycocyanin cy7 tcrβ, by BD (1 mentions) Foxp3 pe, by Thermo Fisher Scientific (1 mentions) Cd4 pe cy7, by BD (1 mentions) Cd45 pe, by BD (1 mentions) Cd3 fitc, by BD (1 mentions) Cd49b fitc, by BD (1 mentions) Il 4 pe, by BD (1 mentions) Cd90 apc, by BD (1 mentions) Cd4 fitc, by BD (1 mentions) Apc conjugated anti mouse cd4, by Thermo Fisher Scientific (1 mentions)

3 protocols using percp cy5.5 cd25

1

Multi-Color Flow Cytometry Analysis of Murine Immune Cells

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All fluorochrome-conjugated antibodies used are listed in “fluorochrome-target” format as follows: FITC-IL-17A, PE-Foxp3, Allophycocyanin-IFN-γ, Allophycocyanin-Cy7-CD4, PerCP-Cy5.5-CD25, PE-Cy5-ICOS, PerCP-eFluor 710-TNF-α, and PE-Cy7-Thy1a were from eBioscience (San Diego, CA); FITC-CD25, FITC-CD45.1, FITC-Thy1a, PECF594-CD4, PE-CF594-CD8α, Allophycocyanin-ICOS, Alexa Fluor 700-CD45.2, PECy7-CD4 and Allophycocyanin-Cy7-TCRβ were from BD Biosciences (San Diego, CA); Alexa Fluor 610-CD4 and PE-Texas Red-CD8α were from Invitrogen (Carlsbad, CA); Brilliant Violet 421-NRP1, Alexa Fluor 700-CD45.1 and PE-Cy7-CD62L were from Biolegend (San Diego, CA), Foxp3 staining buffer kit was from eBiosciences. To detect cytokines, cells were stimulated with PMA/Ionomycin and analyzed as previously described (33 ).
Huang W., Jeong A.R., Kannan A.K., Huang L, & August A. (2014). IL-2 inducible T cell kinase (ITK) tunes T regulatory cell development and is required for suppressive function. Journal of immunology (Baltimore, Md. : 1950), 193(5), 2267-2272.
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2

Quantifying ILC2 and Th2 Cells in Rat Spleens

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On the same day, the rat spleens were removed and the splenic lymphocytes were separated with rat peripheral blood lymphocyte separation solution (Tianjin Haoyang Biological Products Technology Co. Ltd, China) and stained. The proportions of ILC2 and Th2 cells in the spleens were determined using flow cytometry. Lin-CD45 + CD90 + CD25 + cells were identified as ILC2 cells [15 (link)] (Lin includes CD3, CD45R, CD49b, and CD11b/c), and CD4 + IL4 + cells were identified as Th2 cells [16 (link)]. The flow antibodies FITC-CD3, FITC-CD45R, FITC-CD49b, FITC-11b/c, PE-CD45, APC-CD90, APC-cy7-FVS780, PE-IL-4, and FITC-CD4 (BD Biosciences, Franklin Lake, New Jersey, USA), and the flow antibody Percp-cy5.5-CD25 were used (eBioscience, San Diego, California, USA) in the flow cytometry experiments.
Zhu W.X., Xi Y., Li F., Jiao W.E., Li Z.J., Chen S.M., Kong Y.G., Xu Y., Deng Y.Q., Zuo J.J, & Tao Z.Z. (2023). Calpeptin may reverse glucocorticoid-resistance of allergic rhinitis associated with cigarette smoke exposure by down-regulating interferon regulatory factor 1. Heliyon, 9(7), e17316.
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3

Th17/Treg Intracellular Staining Protocol

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For Th17/Treg intracellular staining, cells (1.0 × 10 6 ) were labeled with allophycocyanin (APC)-conjugated anti-mouse CD4 (eBioscience, San Diego, CA, USA) or PerCP Cy5.5-CD25 (eBioscience) monoclonal antibodies (mAbs) at 4
Kobayashi R., Ogawa Y., Hashizume-Takizawa T, & Kurita-Ochiai T. (2020). Oral bacteria affect the gut microbiome and intestinal immunity. Pathogens and disease, 78(3).
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