2 2 diphenyl 1 picrylhydrazyl (dpph)

Manufactured by MedChemExpress

Sourced in United States

DPPH is a stable free radical that is commonly used in antioxidant activity assays. It has a strong absorbance at 517 nm that decreases upon reduction by an antioxidant.

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Lab products found in correlation

4 protocols using 2 2 diphenyl 1 picrylhydrazyl (dpph)

Antioxidant Capacity Evaluation Protocol

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UV–VIS spectrophotometer Helios 3 Thermo Spectronic, differential refractometer Waters 2414 (Mettlet-Toledo, Ltd., Prague, Czech Republic), microjet microwave autoclave (The Rodwell Autoclave Company, London, UK), vortex mixer mini analog (OHAUS Europe GmbH, Nänikon, Switzerland), FTIR Bruker ALPHA (Bruker GmbH, Vienna, Austria), Memmert cultivator (Memmert GmbH + Co. KG, Büchenbach, Germany), Lambda Life laminar box (Lambda Life, Ltd., Bratislava, Slovakia), Waters HPLC Breeze analytical device (Waters Chromatography Europe B.V., Etten-Leur, The Netherlands), OHpak SB-804 HQ analytical column 300 × 8 mm/13µm (Altmann Analytik GmbH & Co. KG, Munich, Germany). 0.1 mol/L phosphate buffer pH 6.8 (Faren Ltd., Uherské Hradiště, Czech Republic), DPPH (2,2-diphenyl-1-picrylhydrazyl) (MedChemExpress, Monmouth Junction, NJ, USA), ABTS (2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (Merck Life Science, Praha, Czech Republic).

Prokopová A., Mokrejš P., Gál R., Pavlačková J, & Hurajová A. (2024). Characterization of Poultry Gelatins Prepared by a Biotechnological Method for Targeted Changes at the Molecular Level. International Journal of Molecular Sciences, 25(2), 916.

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Synthesis and Analysis of Dopamine Derivatives

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Dopamine hydrochloride was purchased from Carbosynth (Berkshire, UK). Trimethyl benzene was purchased from Alfa Aesar (Tewksbury, MA, USA). Morphine, Pluronic F127 (F127), and Tris were purchased from Sigma-Aldrich (St. Louis, MO, USA). DPPH (2,2-Diphenyl-1-picrylhydrazyl) was purchased from Med chem express (Princeton, NJ, USA).

Kuthati Y., Busa P., Tummala S., Rao V.N., Davuluri V.N., Ho Y.P, & Wong C.S. (2021). Mesoporous Polydopamine Nanoparticles Attenuate Morphine Tolerance in Neuropathic Pain Rats by Inhibition of Oxidative Stress and Restoration of the Endogenous Antioxidant System. Antioxidants, 10(2), 195.

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Dopamine-Cy5.5 Conjugate Synthesis

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Dopamine hydrochloride, N, N-dimethylformamide (DMF) and Cy5.5 NHS ester were purchased from Aladdin Reagents of China. Lipopolysaccharide (LPS) was purchased from Sigma-Aldrich. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was purchased from Med chem express. Dihydroethidium (DHE) was purchased from Beyotime Biotechnology. PC-12 cells, Minimum Essential Medium (MEM) and fetal bovine serum (FBS) were achieved from Procell Life Science & Technology.

Zhu T.T., Wang H., Gu H.W., Ju L.S., Wu X.M., Pan W.T., Zhao M.M., Yang J.J, & Liu P.M. (2023). Melanin-like polydopamine nanoparticles mediating anti-inflammatory and rescuing synaptic loss for inflammatory depression therapy. Journal of Nanobiotechnology, 21, 52.

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Antioxidant and Cytotoxicity Assays

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Polyphenolic compounds, 3-(4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT), 2, 2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) disodium salt (ABTS), fluorometric intracellular ROS kit, penicillin/streptomycin, and 2-(4-amidinophenyl)-6-indolecarbami dine dihydrochloride (DAPI) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Parishin derivatives were bought from Chengdu Biopurify Phytochemicals Ltd. (Chengdu, Sichuan, China). Fetal bovine serum (FBS) was obtained from Young in Frontier Company (Seoul, Korea). 2,2-Diphenyl-1-picrylhydrazyl (DPPH) was bought from MedChemExpress (Princeton, NJ, USA). Ham’s F-12 Nutrient Mix medium, BCA protein assay kit, and cDNA synthesis kit (ReverTra Ace qPCR RT Kit) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Eagle’s Minimum Essential Medium (EMEM) was obtained from ATCC (Manassas, VA, USA). H₂O₂ was purchased from Fujifilm Wako Pure Chemical (Osaka, Japan). The SYBR green qPCR Kit was supplied by TOYOBO (Osaka, Japan). Lactate dehydrogenase (LDH) cytotoxicity assay kit was bought from TAKARA (Shiga Prefecture, Japan). Clarity western chemiluminescent (ECL) substrate kit was supplied by Bio-Rad Laboratories (Hercules, CA, USA).

Tian W., Heo S., Kim D.W., Kim I.S., Ahn D., Tae H.J., Kim M.K, & Park B.Y. (2021). Ethanol Extract of Maclura tricuspidata Fruit Protects SH-SY5Y Neuroblastoma Cells against H2O2-Induced Oxidative Damage via Inhibiting MAPK and NF-κB Signaling. International Journal of Molecular Sciences, 22(13), 6946.

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