Tecnai g2 f20 twin cryo tem

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Tecnai™ G2 F20 TWIN Cryo-TEM is a high-resolution transmission electron microscope designed for cryogenic sample imaging. It provides advanced electron optics and a unique TWIN objective lens system to enable high-contrast, low-dose imaging of sensitive biological specimens in their native hydrated state.

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Lab products found in correlation

K alpha x ray photoelectron spectrometer xps system, by Thermo Fisher Scientific (1 mentions) Dxr raman microscope, by Thermo Fisher Scientific (1 mentions) D8 advance, by Bruker (1 mentions) Auriga microscope, by Zeiss (1 mentions) Flexstation 3 reader, by Molecular Devices (1 mentions) Nano zs instrument, by Malvern Panalytical (1 mentions) X maxn 80t, by Oxford Instruments (1 mentions)

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Tecnai G2 F20 (520 citations) Tecnai F20 (449 citations) Tecnai G2 (325 citations) Tecnai F20 TEM (70 citations) Tecnai G2 TEM (42 citations) Tecnai G2 F20 TEM (29 citations) Tecnai G2 Twin TEM (14 citations) Cryo-TEM (6 citations) Cryo Tecnai F20 (4 citations) Tecnai F20 cryo-TEM (2 citations)

6 protocols using tecnai g2 f20 twin cryo tem

Characterization of Ru-doped TiO2 Nanomaterials

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X-Ray diffraction (XRD) of the samples was analyzed at ambient temperature on a Bruker D8 Advance diffractometer using the characteristic Kα radiation of copper at a voltage of 40 kV and a current of 40 mA. XRD patterns were collected in the 2θ range between 5° and 80°.
The UV-vis diffuse reflectance spectra of rutile Ru_xTi_1-xO₂ were obtained by using a Jasco UV-Vis V-550 spectrophotometer in the wavelength range from 200 to 900 nm with an integrating sphere assembly. The sample was diluted with MgO (ratio 1:6) and then mechanically mixed. The UV-vis absorption was transformed according to the Kubelka Munk function,

F (R_{\infty})

, for infinite thick samples. The sample surface elements and their oxidation states were analyzed by Thermo Scientific K-Alpha X-ray Photoelectron Spectrometer (XPS) system with Al K-alfa radiation. The Raman spectra of samples were registered using a DXR Raman Microscope from Thermo Scientific. The morphology of the samples was characterized by transmission electron microscope Tecnai™ G2 F20 TWIN Cryo-TEM, FEI Company™, through bright field (BF-TEM) and scanning transmission electron microscopy analyses. The TEM was operated under an acceleration voltage of 200 kV. A small drop of well-dispersed sample, ultrasonicated for 5 minutes, was put on a carbon film copper grid and then, visualized on TEM.

Mihai S., Cursaru D.L., Matei D., Manta A.M., Somoghi R, & Branoiu G. (2019). Rutile RuxTi1-xO2 nanobelts to enhance visible light photocatalytic activity. Scientific Reports, 9, 18798.

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Nanoparticle Morphological Analysis

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The evaluation of the morphological features of the nanoparticles was realized using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Examination of the surface and cross-sectional morphology of the samples surface were performed using Thermo Scientific™ FEI Quanta™ 650 FEG variable-pressure and environmental high-performance scanning electron microscope ((Hillsboro, OR, USA, SEM with Field Emission Gun). Transmission electron microscopy images were recorded using Tecnai G2 F20 TWIN Cryo-TEM (FEI Company, Hillsboro, OR, USA), at 300 kV acceleration voltage and a resolution of 1Å.

Ungureanu C., Fierascu I., Fierascu R.C., Costea T., Avramescu S.M., Călinescu M.F., Somoghi R, & Pirvu C. (2021). In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts. Materials, 14(8), 1845.

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Morphological Analysis of Cu-Au NPs

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The morphology of the Cu-Au NPs was examined using transmission electron microscopy (TECNAI G2 F20 TWIN Cryo-TEM, FEI Company, Hillsboro, OR, USA) with an accelerating voltage of 200 keV. A small droplet from both dispersions of Cu-Au (1:1) NPs (just after irradiation and after 1 year) was placed on copper grids with Lacey carbon film, without staining.

Lungulescu E.M., Setnescu R., Pătroi E.A., Lungu M.V., Pătroi D., Ion I., Fierăscu R.C., Șomoghi R., Stan M, & Nicula N.O. (2021). High-Efficiency Biocidal Solution Based on Radiochemically Synthesized Cu-Au Alloy Nanoparticles. Nanomaterials, 11(12), 3388.

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Synthesis and Characterization of Silicon Quantum Dots

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The SiQDs used within this study were obtained at the National Institute for Laser, Plasma and Radiation Physics, Bucharest, Magurele, Romania, by the method described in 2005 by Grigoriu et al. using ablation in a stainless steel chamber [22 ]. Briefly, the laser beam was focused on a silicon target inside the chamber; the nanoparticles were taken up by the gas flow and directed to a Millipore filter with a pore size of 100 nm, reacted with oxygen in the air, formed a silicon dioxide layer on their surface, and resulted in core/shell QDs of silicon/silica.
The SiQDs were analyzed by scanning electron microscopy (SEM) on a Zeiss Auriga microscope (Carl Zeiss, Jena, Germany), acquiring the images at a voltage of 5 kV, and in a bright-field mode of transmission electron microscopy (TEM) using a Tecnai F20 G2 TWIN Cryo-TEM (FEI, Hillsboro, OR, USA) at an acceleration voltage of 200 kV.
The measurement of the hydrodynamic size, polydispersity index, and zeta potential of SiQDs dispersed in saline was performed in triplicate at 25 °C using a refractive index of 1.52 on a Malvern Nano-ZS instrument (Malvern Instruments, Malvern, Worcestershire, UK). The absorbance and emission spectra of SiQDs were recorded in 0.9% NaCl solution using the FlexStation 3 reader (Molecular Devices, San Jose, CA, USA).

Cristian R.E., Balta C., Herman H., Trica B., Sbarcea B.G., Hermenean A., Dinischiotu A, & Stan M.S. (2024). In Vivo Assessment of Hepatic and Kidney Toxicity Induced by Silicon Quantum Dots in Mice. Nanomaterials, 14(5), 457.

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Cryo-TEM Analysis of 3D Printed Composites

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The freeze-dried 3D printed composites were examined using transmission electron microscopy (TEM). On a TECNAI F20 G2 TWIN Cryo-TEM (FEI, Hillsboro, OR, USA), all of the samples were examined in BF-TEM (Bright Field Transmission Electron Microscopy) mode at an accelerating voltage of 200 kV.

Marin M.M., Gifu I.C., Pircalabioru G.G., Albu Kaya M., Constantinescu R.R., Alexa R.L., Trica B., Alexandrescu E., Nistor C.L., Petcu C, & Ianchis R. (2023). Microbial Polysaccharide-Based Formulation with Silica Nanoparticles; A New Hydrogel Nanocomposite for 3D Printing. Gels, 9(5), 425.

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Biogenic Selenium Nanoparticles Characterization

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Biogenic selenium nanoparticles were visualized by TEM using TECNAI F20 G2 TWIN Cryo-TEM (FEI) transmission electron microscope (Houston, TX, USA), and the elemental composition was assessed using the EDX detector (X-MaxN 80T—Oxford Instruments, Abingdon, Oxfordshire, UK).

Tritean N., Dima Ș.O., Trică B., Stoica R., Ghiurea M., Moraru I., Cimpean A., Oancea F, & Constantinescu-Aruxandei D. (2023). Selenium-Fortified Kombucha–Pollen Beverage by In Situ Biosynthesized Selenium Nanoparticles with High Biocompatibility and Antioxidant Activity. Antioxidants, 12(9), 1711.

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