Uv 1800 spectrometer

Manufactured by Shimadzu

Sourced in Japan, Switzerland

The UV-1800 spectrometer is a compact and versatile laboratory instrument designed for ultraviolet and visible spectroscopy. It features a double-beam optical system and a wide wavelength range of 190 to 1100 nanometers. The UV-1800 provides accurate and reliable measurements for various applications that require spectroscopic analysis.

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Close spelling variants of this product

UV-1800 (1915 citations) UV-1800 UV-vis spectrometer (15 citations) UV spectrometer (UV-1800, (2 citations) 1800 spectrometer (2 citations) Spectrometers (2 citations) UV-1800 absorption spectrometer (2 citations) DR 1800 UV-visible spectrometer (2 citations)

118 protocols using uv 1800 spectrometer

Adsorption Isotherm of Dopamine on Gold Nanoparticles

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To construct the adsorption isotherm of DA on GNPs, UV-vis studies were performed to accurately measure the uptake of DA by GNPs. A certain amount of GNPs were mixed with a defined unit volume (1.0 mL) of different concentrations of DA solutions. The amount of GNPs used for each DA solution was estimated by calculation such that the GNPs used must adsorb at least 20% of DA in solution, assuming a full monolayer coverage of DA on GNPs. The mixture of GNPs and DA solution was then sonicated (FB15050, Fisher Scientific, 50/60 Hz, 80 W, Germany) for 35 min to allow full adsorption, followed by centrifugation (Eppendorf Centrifuge 5430 R) for 10 min at 14 000 rpm. The original DA solution prior to adsorption and the supernatant after adsorption then centrifugation were both diluted into the calibration region then examined by UV-vis spectroscopy.
UV-vis spectroscopy was conducted using a Shimadzu spectrometer UV-1800 and quartz cells with a 10 mm optical path. In all cases, a baseline correction was conducted prior to any measurement, and the absorbance was recorded from 400–220 nm.

Chen L., Tanner E.E., Lin C, & Compton R.G. (2017). Impact electrochemistry reveals that graphene nanoplatelets catalyse the oxidation of dopamine via adsorption †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc03672h. Chemical Science, 9(1), 152-159.

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Electrochemical and UV-vis Characterization

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UV-vis spectroscopy was conducted using a Shimadzu spectrometer UV-1800 and quartz cells with a 10 mm optical path. The absorbance was recorded from 800-300 nm and a baseline correction was conducted prior to all measurements.
All electrochemical experiments were conducted with a standard three-electrode system in a Faraday cage at 298K. The cyclic voltammetric measurements were performed using a Autolab II potentiostat (Metrohm-Autolab BV, Netherlands). Semi-circular potential wave sweep voltammetry was carried out with a computer-controlled in-lab built potentiostat ensuring the low-noise measurements with signal sampled at a stream rate of 100 kHz (Amin et al., 2019) (link). The potentiostat was controlled by script written in Python 3.5 to generate required potential waveform. For the voltammetric measurements, a glassy carbon (GC) macroelectrode (diameter calibrated as 2.99 mm) was used as the working electrode, a saturated calomel electrode (SCE, ALS distributed by BASi Inc., Japan.) as the reference electrode and a graphite rod as the counter electrode. The GC electrode was polished onto the soft lapping pads (Buehler, UK) with alumina (particles size of 1.0 and 0.3 m, Buehler, IL, UK) before each voltammetric experiment, followed by sonication in water and drying with nitrogen.

Wang Y., Chen L, & Compton R.G. (2020). Selective voltammetric detection of chlorophylls using a semi-circular potential wave. Food chemistry, 323.

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Determination of Enzymatic Antioxidants

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Fresh leaf samples were collected from the topmost part of the plant after treatment for 21 days and used for the determination of enzymatic antioxidants. The activity of the antioxidant enzymes, namely SOD, POD, CAT, and APX was determined spectrophotometrically (1800 UV Spectrometer, Shimadzu). Leaf samples (1.0 g) were thoroughly crushed using pre-cooled mortar and pestle and then homogenised in 10 mL of 0.05 M phosphate buffer (pH 7.8), filtered using muslin cloth and centrifuged at 10,000 ×g for 15 min at 4 °C. Thereafter, the enzyme extract was used to evaluate SOD and POD activity using the method reported by Zhang [42 ].
CAT activity was measured according to Aebi [43 (link)]. Assay samples (3 mL) consisted of enzyme extract (100 μL), 300 mM H₂O₂ (100 μL), and 50 mM phosphate buffer (2.8 mL, pH 7.0) with 2 mM Ca. CAT activity was then determined by measuring the decrease in absorbance at 240 nm as a consequence of H₂O₂. APX activity was measured according to the procedures of Nakano and Asada [44 (link)]. APX activity was estimated by monitoring the change in the absorbance at 290 nm.

Singh D., Sharma N.L., Singh C.K., Sarkar S.K., Singh I, & Dotaniya M.L. (2020). Effect of chromium (VI) toxicity on morpho-physiological characteristics, yield, and yield components of two chickpea (Cicer arietinum L.) varieties. PLoS ONE, 15(12), e0243032.

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Proline Content Determination Protocol

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Proline content was estimated according to the method of Bates et al. [41 (link)]. Fresh root and shoot tissue samples (0.5 g) were collected and homogenised in 3% aqueous sulphosalicylic acid filtered through Whatman filter paper (Praxor, Tamil Nadu, India). Then, 2 mL of glacial acetic acid and 2 mL of ninhydrin were added to the filtered extract (2mL) and boiled in a water bath at 100 °C for 1 h before extraction with 4 mL of toluene. To evaluate the proline content, the absorbance of the chromophore phase was measured at 520 nm using a 1800 UV Spectrometer (Shimadzu Corp. Kyoto, Japan). The quantity of proline was expressed as μM g⁻¹ fresh weight (FW).

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Chlorophyll Quantification in Plant Leaves

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After 95 days of treatment, 0.5 g samples of healthy leaves from the upper portions of plants were used to estimate Chl a and Chl b contents. The Chl was extracted in 85% v/v acetone solution (Sigma Aldrich, St. Louis, MI, USA) and kept in the dark at 4 °C until the green colour disappeared. The extracts were then centrifuged at 3000×g for 10 min at 4 °C, and the absorbance of the supernatant was recorded at 663 nm and 645 nm for Chl a and Chl b, respectively, using a spectrophotometer (1800 UV Spectrometer, Shimadzu) [45 (link)]. Chl content was determined according to Arnon [46 (link)] and expressed as mg g⁻¹ FW. The total Chl content was calculated as follows:

Total Chl (mg g - 1 FW) = 20.2 (OD at 663 nm) + 8.02 (OD at 645 nm) \times \frac{V}{1000 \times W},

where V is the final volume, OD is the optical density, and W is the fresh weight of samples.

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Spectroscopic Analysis of Stavudine Solutions

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Aqueous solutions of buffer phosphate was prepared by mixing a particular volume of KH2PO4 with a concentration of 0.0667 M ,then the volume completed with Na2HPO4 2H2O with a concentration of 0.0667M to 100 mL, followed by PH adjusting . Aqueous solutions of Stavudine (D4T) with a concentration of 1 x10 -4 M was prepared as a stock solution. Spectroscopic measurements was implemented at 37 0 C for drug (1x10 -5 M) solution , n-Hexane and drug solution in Sodium dodecyl sulfate micelle(with respect to critical micelle concentration in the preparation process ) using Shimadzu 1800 UV-spectrometer in the range of 200-400 nm.

Determination of Chemical Potential for Stavudine (D4T) Diffusion through SDS Micelle Solution. (2020). Systematic Reviews in Pharmacy, 11(06).

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Quantifying DsiRNA Entrapment in AuNPs

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The entrapment efficiency (EE) of DsiRNA loaded onto AuNPs was measured using a UV–visible spectrophotometer (UV spectrometer 1800, Shimadzu, Kyoto, Japan). DsiRNA-AuNPs were centrifuged at 10000 rpm for 30 min (Universal 320 R ultracentrifuge, Andreas Hettich GmbH & Co., Germany). The absorbance (A) of supernatant recovered from centrifugation was measured at 260 nm and scanned at 480 mm/min. The DsiRNA entrapment efficiency was calculated using the formula below:

D s i R N A E E (%) = \frac{(A b s o r b a n c e o f D s i R N A a d d e d - A b s o r b a n c e o f D s i R N A i n s u p e r n a t a n t)}{(A b s o r b a n c e o f D s i R N A a d d e d)} \times 100

The binding efficiency of DsiRNA onto the AuNPs surfaces was determined using E-Gel^TM 4% agarose stained with ethidium bromide (Invitrogen, USA). Naked DsiRNA was used as positive control while 10 bp DNA ladder (Invitrogen, USA) as a size reference. Approximately, 20 µL sample (containing different concentrations of AuNPs-CLRE and AuNPs-HLRE loaded with 15 µg/mL of DsiRNA) were loaded in each well. Electrophoresis were run for 30 min according to E-Gel™ protocol on E-Gel™ Power Snap Electrophoresis Device (Thermofisher Scientific).

Nor Azlan A.Y., Katas H., Habideen N.H, & Mh Busra M.F. (2020). Dual-action of thermoresponsive gels containing DsiRNA-loaded gold nanoparticles for diabetic wound therapy: Characterization, in vitro safety and healing efficacy. Saudi Pharmaceutical Journal : SPJ, 28(11), 1420-1430.

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Bacterial Cell Preparation for Microfiltration

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For all experiments, the bacterial strains were revitalised and maintained on standard tryptic–soy–agar (TSA, tryptone 15 g/L, soy peptone 5 g, NaCl 5 g/L, agar 15 g/L, pH 7.2) anfd incubated at 37 ± 0.5 °C for 24 h (E. coli) to 48 h (S. aureus and M. luteus). Individual colonies were then inoculated into 50 mL of tryptic–soy–broth (TSB) and incubated overnight (for 16 h E. coli and S. aureus; and for 18 h M. luteus) at 37 ± 0.5 °C on a rotary shaker (Innova 230, New Brunswick Scientific, Enfield, CT, USA) at 180 rpm. The ice-cooled overnight bacterial biomass was harvested by centrifugation at 6000 rpm and 4 ± 0.5 °C for 5 min. The cell pellet was rinsed twice by resuspension in deionised water, followed by another centrifugation. The final pellet was then resuspended in deionised water and the optical density was measured at 600 nm (UV Spectrometer 1800, Shimadzu, Kyoto, Japan). The bacterial cell concentration was adjusted to ~10⁸ CFU/mL. For the microfiltration experiments, a calibrated suspension of bacterial cells was diluted 100 times in the required volume of deionised water. The final concentration of bacterial cells in the feed suspension was ~10⁶ CFU/mL. Its homogeneity throughout the experiment was maintained using a magnetic stirrer.

Kokol V., Kos M., Vivod V, & Gunde-Cimerman N. (2023). Cationised Fibre-Based Cellulose Multi-Layer Membranes for Sterile and High-Flow Bacteria Retention and Inactivation. Membranes, 13(3), 284.

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Analytical Techniques for Natural Product Characterization

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All the 1D and 2D NMR spectra were obtained by a Bruker AVANCE III 600 MHZ spectrometer with TMS as an internal standard (Bruker company, Switzerland). The HRESIMS data were obtained on an Agilent 6210 TOF MS system (Agilent Technologies, Santa Clara, CA, USA) or AB SCIEX Triple TOF 5600⁺ (AB SCIEX, USA). Optical rotations were measured by a JASCO P-1020 polarimeter (Jasco Tokyo Japan). UV spectra were performed in MeOH by using a Shimadzu UV spectrometer-1800 (Shimadzu Corp., Kyoto, Japan). IR spectra (KBr) were obtained on a Nicolet 6700 FT-IR spectrometer (Thermo Electric Nicoli, United States). Semipreparative high performance liquid chromatography (HPLC) was performed by an Agilent 1260 separation system with an Aglient ZORBAC SB-C₁₈ column (5 μm, 250 mm × 9.4 mm, 3 mL/min). Sephadex LH-20 gel (GE Healthcare, Uppsala, Sweden) and MCI gel (Mitsubishi Chemical Corp., Japan) were used in column chromatography. And silica gel (200–300 mesh for column chromatography, GF254 for TLC) was supplied by the Yantai Zhifu Huanwu Silicone Factory, Yantai, China.

Lv H., Su H., Xue Y., Jia J., Bi H., Wang S., Zhang J., Zhu M., Emam M., Wang H., Hong K, & Li X.N. (2023). Polyketides with potential bioactivities from the mangrove-derived fungus Talaromyces sp. WHUF0362. Marine Life Science & Technology, 5(2), 232-241.

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Quantitative UV/Vis Analysis of Analyte

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UV/Vis spectrophotometry was employed as the reference method, by measuring the absorbance of DS at λ_max = 280 nm, as described by Fabre et al. (1993) [46 (link)]. A stock standard solution of DS (60 mg/L) was prepared by dissolving 3 mg of standard in 50 mL of solvent mixture (methanol–water, 1:1 v/v). Serial dilutions were performed for the preparation of calibration standards containing 5–15 mg/L DS. These solutions were measured in the range 200–500 nm and the calibration curve was constructed by using their absorbance at λ_max. The measurements were conducted using a UV-1800 spectrometer (Shimadzu, Kyoto, Japan). The known samples that were prepared with the aforementioned procedure (§ 3.3) were further diluted with the solvent mixture, in order for their final concentrations to fall within the calibration curve concentration range.

Siozou E., Sakkas V, & Kourkoumelis N. (2021). Quantification and Classification of Diclofenac Sodium Content in Dispersed Commercially Available Tablets by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis. Pharmaceuticals, 14(5), 440.

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