Uas mcherry rnai

Manufactured by Bloomington Drosophila Stock Center

The UAS-mcherry RNAi is a genetic tool used for RNA interference (RNAi) in Drosophila. It contains the mCherry fluorescent protein gene under the control of a UAS promoter, which can be used to induce RNAi-mediated gene silencing in a targeted manner.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

UAS-mCherry (9 citations) MCherry RNAi (6 citations)

12 protocols using uas mcherry rnai

Drosophila TREM2 and TYROBP Models

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were maintained in standard cornmeal media at 25 °C. Complementary DNA (cDNA) encoding the full length of TREM2 (NM_018965, RC221132) and TYROBP (NM_198125, RC203771) with Myc-DDK tag were obtained from OriGene Technologies, Inc. These constructs were subcloned into a pJFRC19-13XLexAop2 vector (Addgene #26224). TREM2R47H mutation was introduced by using site-directed mutagenesis kit (Takara Bio Inc.). Transgenic flies were generated by PhiC31 integrase-mediated transgenesis systems (Best Gene Inc.). Transgenic fly lines carrying UAS-Aβ42 and UAS-tau were previously described [43 (link)–45 (link)]. Repo-LexA (#67096), Elav-GAL4 (#458), GMR-GAL4 (#1104), UAS-para RNAi (#31626), and UAS-mcherry RNAi (#35785) were obtained from the Bloomington Stock Center. For RNA sequencing (RNA-seq), around seven-day-old male flies were used. All experiments were performed using age-matched male flies.

Sekiya M., Wang M., Fujisaki N., Sakakibara Y., Quan X., Ehrlich M.E., De Jager P.L., Bennett D.A., Schadt E.E., Gandy S., Ando K., Zhang B, & Iijima K.M. (2018). Integrated biology approach reveals molecular and pathological interactions among Alzheimer’s Aβ42, Tau, TREM2, and TYROBP in Drosophila models. Genome Medicine, 10, 26.

+ Open protocol

+ Expand

Visualizing Dendrites and Transgene Expression

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Detailed genotypes of the animals and clones are described and summarized in Supplementary Table S1. To visualize dendrites and /or express transgenes, we used the following Gal4 drivers: Gr28b.c51 (link)52 (link) and Gal4^5-4053 (link). To express fluorescent proteins, we used UAS-mCD8:GFP (#5137 of the Bloomington Stock Center) or UAS-Venus-pm44 (link)54 (link)55 (link). Other strains used were dinr³³⁹56 (link), Akt^q57 (link), Tor^ΔP58 (link), rok²59 (link), UAS-dicer2 (#60009 of Vienna Drosophila RNAi Center), UAS-raptor^RNAi (HMS00124/#34814 of the Bloomington Stock Center), UAS-mCherry^RNAi (#35785 of the Bloomington Stock Center), UAS-CHORD^RNAi (this study), UAS-Dp110[D954A] (#25918 of the Bloomington Stock Center), UAS-rok^RNAi (GD1522/#3793 and KK107802/#104675 of the Vienna Drosophila RNAi Center; JF03225/#28797, HMS01311/#34324, and GL00209/#35305 of the Bloomington Stock Center), UAS-hsp90^RNAi (HMS00899/#33947 of the Bloomington Stock Center) and UAS-Rok.CAT^48.260 (link).

Shimono K., Fujishima K., Nomura T., Ohashi M., Usui T., Kengaku M., Toyoda A, & Uemura T. (2014). An evolutionarily conserved protein CHORD regulates scaling of dendritic arbors with body size. Scientific Reports, 4, 4415.

+ Open protocol

+ Expand

Drosophila Genetic Toolkit Protocols

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were maintained in standard cornmeal media at 25°C. Transgenic fly line carrying UAS-human tau was previously described.⁹⁵ (link) Canton-S line is a kind gift from Dr. K. Akagi (Toyama University). Toll-9 KO fly is a kind gift from Dr. J. Royet (Aix-Marseille Université).³⁵ (link) UAS-Toll-9 and the background strain y¹w^67c23 line (used as a control for UAS-Toll-9) are kind gifts from Dr. Y. Yagi (Nagoya University).³⁰ (link) Or85e-mCD8::GFP fly is a kind gift from Dr. M.R. Freeman (Oregon Health & Science University).⁵¹ (link) Repo-GAL4 (X) driver line and drpr^Δ5mutant fly are kind gifts from Dr. T. Awasaki (Kyorin University).¹⁰⁵ (link)^,¹⁰⁶ (link) The GMR-GAL4 (#1104), Rh1-GAL4 (#8688), GeneSwitch GMR-GAL4 (#6759), repo-GAL4 (III) (#7415), UAS-mCherry RNAi (#35785), UAS-Toll-9 RNAi #1 (#30535), UAS-Toll-9 RNAi #2 (#34853), UAS-DN-p38b (#59005) and attP2 background line (#36303) were obtained from the Bloomington Stock Center. The UAS-Luciferase transgenic flies were generated by PhiC31 integrase-mediated transgenesis systems (Best Gene Inc.). Experiments were performed using age-matched male or female flies. Genotypes and ages of all flies used in this study are described in Table S1.

Sakakibara Y., Yamashiro R., Chikamatsu S., Hirota Y., Tsubokawa Y., Nishijima R., Takei K., Sekiya M, & Iijima K.M. (2023). Drosophila Toll-9 is induced by aging and neurodegeneration to modulate stress signaling and its deficiency exacerbates tau-mediated neurodegeneration. iScience, 26(2), 105968.

+ Open protocol

+ Expand

Drosophila Neurodegeneration Model Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were maintained in standard cornmeal media at 25 °C. Transgenic fly lines carrying UAS-Aβ42 and UAS-Tau were previously described (Iijima et al., 2004 (link); Sekiya et al., 2017 (link)). The elav-GAL4 (#458), GMR-GAL4 (#1104), UAS-mcherry RNAi (#35785), UAS-Vha68–1 RNAi (#50726 and #42888), Vha68–1¹ (#82466), and UAS-Vha68–2 RNAi (#34582) were obtained from the Bloomington Drosophila Stock Center. UAS-Vha68–1 RNAi (#46397) and UAS-Vha68–2 RNAi (#110600) were obtained from the Vienna Drosophila Resource Center. The UAS-Luciferase RNAi Transgenic flies were generated by PhiC31 integrase-mediated transgenesis systems (Best Gene Inc.). Genotypes and ages of all flies used in this study are provided in figure legends. Experiments were performed using age-matched male flies and genetic background of the flies was controlled. For example, for RNAi experiments, we crossed virgin females from elav-GAL4; UAS-Aβ42 (double transgenic flies expressing Aβ42 pan-neuronally) and males from UAS-Vha68–1 RNAi lines (experimental group) or a UAS-mcherry RNAi line with the same genetic background as the RNAi lines (control group). The resultant offspring from each cross has the same hybrid genetic background and these flies were used for the experiments.

Wang M., Li A., Sekiya M., Beckmann N.D., Quan X., Schrode N., Fernando M.B., Yu A., Zhu L., Cao J., Lyu L., Horgusluoglu E., Wang Q., Guo L., Wang Y.S., Neff R., Song W.M., Wang E., Shen Q., Zhou X., Ming C., Ho S.M., Vatansever S., Kaniskan H.Ü., Jin J., Zhou M.M., Ando K., Ho L., Slesinger P.A., Yue Z., Zhu J., Katsel P., Gandy S., Ehrlich M.E., Fossati V., Noggle S., Cai D., Haroutunian V., Iijima K.M., Schadt E., Brennand K.J, & Zhang B. (2020). Transformative Network Modeling of Multi-Omics Data Reveals Detailed Circuits, Key Regulators, and Potential Therapeutics for Alzheimer’s Disease. Neuron, 109(2), 257-272.e14.

+ Open protocol

+ Expand

Drosophila Genetics: Diverse Lines and Tools

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were maintained on a cornmeal–molasses–yeast medium and at room temperature (22°C) with 60–65% humidity. The following Drosophila lines were obtained from the Bloomington Stock Center: UAS-LacZ, UAS-mCherry-RNAi, UAS-emb-RNAi, GMR-GAL4, and OK371-GAL4. The UAS-p53.ORF.3xHA was obtained from FlyORF. The UAS-(G4C2)30 lines were obtained from Dr Peng Jin’s laboratory (Xu et al., 2013 (link)). The three p53 RNAi lines used in this study are summarized in the Key Resources Table.

Maor-Nof M., Shipony Z., Lopez-Gonzalez R., Nakayama L., Zhang Y.J., Couthouis J., Blum J.A., Castruita P.A., Linares G.R., Ruan K., Ramaswami G., Simon D.J., Nof A., Santana M., Han K., Sinnott-Armstrong N., Bassik M.C., Geschwind D.H., Tessier-Lavigne M., Attardi L.D., Lloyd T.E., Ichida J.K., Gao F.B., Greenleaf W.J., Yokoyama J.S., Petrucelli L, & Gitler A.D. (2021). p53 is a central regulator driving neurodegeneration caused by C9orf72 poly(PR). Cell, 184(3), 689-708.e20.

+ Open protocol

+ Expand

Drosophila Alzheimer's Disease Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were maintained in standard cornmeal media at 25°C. Elav-GAL4 (#458), UAS-mcherry RNAi (#35785) and UAS-Rac1 RNAi (#34910 and #28985) transgenic flies were obtained from the Bloomington Stock Center. Transgenic fly line carrying UAS-Aβ42 was previously described (35 (link)). Experiments were performed using age-matched male flies. The ages of the flies used in the experiments presented in Figure 5A and Supplementary Material, Figure S5A were 7 days old, and the ages of the other flies are indicated in the figures. The genotypes of the flies were as follows: (mcherry RNAi): Elav-GAL4/Y;;UAS-mcherry RNAi/+, (Rac1 RNAi): Elav-GAL4/Y;;UAS-Rac1 RNAi #34910/+, (Control): Elav-GAL4/Y, (Aβ42): Elav-GAL4/Y;UAS-Aβ42/+;, (Aβ42/mcherry RNAi): Elav-GAL4/Y;UAS-Aβ42/+; UAS- mcherry RNAi/+, (Aβ42/Rac1 RNAi): Elav-GAL4/Y;UAS-Aβ42/+; UAS- Rac1 RNAi #34910/+ and (Rac1 RNAi (#28985)): Elav-GAL4/Y;;UAS-Rac1 RNAi #28985/+.

Kikuchi M., Sekiya M., Hara N., Miyashita A., Kuwano R., Ikeuchi T., Iijima K.M, & Nakaya A. (2020). Disruption of a RAC1-centred network is associated with Alzheimer’s disease pathology and causes age-dependent neurodegeneration. Human Molecular Genetics, 29(5), 817-833.

+ Open protocol

+ Expand

Drosophila Genetics: Protein Regulation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were kept at 25°C for all experiments. Fly stocks were UAS-Mfap1 (FlyORF F001460), UAS-Mfap1 RNAi (VDRC 15610), UAS-coilin::YFP [16 (link)], ppk-GAL4 [21 (link)], UAS-tdtomato [22 (link)], UAS-VCP QQ [9 (link)], UAS-mcherry RNAi (Bloomington 35785).

Rode S., Ohm H., Zipfel J, & Rumpf S. (2017). The spliceosome-associated protein Mfap1 binds to VCP in Drosophila. PLoS ONE, 12(8), e0183733.

+ Open protocol

+ Expand

Drosophila Genetics Toolbox Utilization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following stocks were obtained from Tsinghua RNAi Stock Center: UAS-babo-RNAi (THU5256), UAS- uba1-RNAi (THU2127).
The following stocks were obtained from Bloomington Stock Center (BSC): w1118, ok6-gal4, elav-gal4 (c155), PPK-Gal4, mCD8-GFP, UAS-EcR-RNAi (BSC9327), UAS-EcR-B1^DN (BSC9452), UAS-bsk-RNAi (BSC36643), UAS-mCherry-RNAi (BSC35785).
UAS-tubulin-GFP (Dr. Xing Liang, University of Tsinghua).

Xu W., Kong W., Gao Z., Huang E., Xie W., Wang S, & Rui M. (2023). Establishment of a novel axon pruning model of Drosophila motor neuron. Biology Open, 12(1), bio059535.

+ Open protocol

+ Expand

Drosophila Model of Tau Pathology

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were maintained in standard cornmeal media at 25°C. The transgenic fly lines carrying the human 0N4R tau, which has four tubulin-binding domains (R) at the C-terminal region and no N-terminal insert (N), include a kind gift from Dr. M. B. Feany (Harvard Medical School) [59 (link)] and the lines established following the standard method [63 (link), 114 (link)] by using human 0N4R tau (a kind gift from Dr Mike Hutton (Mayo Clinic Jacksonville)). The UAS-Aβ42 and UAS-luciferase RNAi transgenic flies were previously described [63 (link), 64 (link), 114 (link)]. The transgenic fly line carrying UAS-S2Atau was established following the standard method [63 (link), 114 (link)] by using human 0N4R tau (a kind gift from Dr Mike Hutton (Mayo Clinic Jacksonville)) with alanine mutation at Ser262 and Ser356 introduced by using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA, USA). The elav-GAL4, GMR-GAL4, UAS-CD8GFP were obtained from the Bloomington Stock Center. UAS-PAR-1 RNAi is a gift from Dr. J. McDonard (Cleveland Clinic, Cleveland, USA) [70 (link)]. UAS-PAR-1 is a gift from Dr. Bingwei Lu (Stanford University) [115 (link)]. UAS-Sgg RNAi, UAS-mCherry RNAi (TRiP at Harvard Medical School) were obtained from Bloomington stock center. All experiments were performed using age-matched male flies, and genotypes are described in S1 Table.

Ando K., Maruko-Otake A., Ohtake Y., Hayashishita M., Sekiya M, & Iijima K.M. (2016). Stabilization of Microtubule-Unbound Tau via Tau Phosphorylation at Ser262/356 by Par-1/MARK Contributes to Augmentation of AD-Related Phosphorylation and Aβ42-Induced Tau Toxicity. PLoS Genetics, 12(3), e1005917.

+ Open protocol

+ Expand

Drosophila Neurodegeneration Research Protocols

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Flies were maintained in standard cornmeal media at 25°C. Transgenic fly lines carrying UAS-Luciferase RNAi, UAS-human tau and UAS-Aβ42 were previously described [43 (link),63 (link)]. Eaat1^SM2 and a precise excision line Eaat1^PE (used as a control line for Eaat1^SM2) are kind gifts from Dr. D. J. van Meyel (McGill University) [55 (link)]. The elav-GAL4 (#458), Repo-GAL4 (#7415), GMR-GAL4 (#1104), UAS-wfs1 RNAi^TRiP (#53330), UAS-mcherry RNAi (#35785), wfs1^e03461 (#18157), wfs1^MI14041 (#59250), UAS-wfs1 (#8356), UAS-wfs1 (#8357), UAS-mitoGFP (#8442) and UAS-Luciferase (#35788), Opa1^S3475 (#12188) were obtained from the Bloomington Stock Center. UAS-wfs1 RNAi^GD (#42664) was obtained from the Vienna Drosophila RNAi Center. wfs1^LL07290 (#142011) was obtained from Kyoto Stock Center. Genotypes and ages of all flies used in this study are provided in S1 Table. Experiments were performed using age-matched male or female flies.

Sakakibara Y., Sekiya M., Fujisaki N., Quan X, & Iijima K.M. (2018). Knockdown of wfs1, a fly homolog of Wolfram syndrome 1, in the nervous system increases susceptibility to age- and stress-induced neuronal dysfunction and degeneration in Drosophila. PLoS Genetics, 14(1), e1007196.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!