Anti wt1 sc 192

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-Wt1 (sc-192) is a laboratory antibody product produced by Santa Cruz Biotechnology. It is designed to detect the Wt1 (Wilms' Tumor 1) protein in various assays.

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Lab products found in correlation

Ab10543, by Abcam (1 mentions) Antibody diluent with background reducing components, by Agilent Technologies (1 mentions) Goat anti rat igg h l alexafluor 488, by Thermo Fisher Scientific (1 mentions) Protein block serum free, by Agilent Technologies (1 mentions) Alexa fluor 488 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Biorevo microscope, by Keyence (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Ab15251, by Abcam (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti β catenin, by BD (1 mentions) Anti β actin mouse monoclonal antibody a5316, by Merck Group (1 mentions) Tnf α 315 01a, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Zhongshan Biotechnology (1 mentions)

Close spelling variants of this product

WT1 (sc-192; Sc-192 Anti-WT1

4 protocols using anti wt1 sc 192

Comprehensive Kidney Development Analysis

Cited 1 time

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Histology, immunohistochemistry, and in situ hybridization were carried out according to standard procedures. Briefly, kidneys were fixed in 4% paraformaldehyde (PFA) for overnight at 4°C, dehydrated, and embedded in wax or OCT. Paraffin or frozen sections were generated at 6 μm of thickness. We used six embryos for each genotype at each stage for each probe and the result was consistent in each embryo. Probes for ISH were reported previously (Xu et al., 2014 (link)). Cy3-, Cy2-, Cy5-and FITC-conjugated secondary antibodies were used and Hoechst was used for nuclear counter-staining.
Primary antibodies: Anti-Six1 (12,891, Cell Signaling), -Six2 (MBS610128, MyBiosource), anti-Wt1 (sc-192, Santa Cruz Biotechnology), -Eya1 (25-067, Prosci Inc. and MABE1047, Sigma), and -PH3 (ab10543, Abcam).

Xu J., Li J., Ramakrishnan A., Yan H., Shen L, & Xu P.X. (2022). Six1 and Six2 of the Sine Oculis Homeobox Subfamily are Not Functionally Interchangeable in Mouse Nephron Formation. Frontiers in Cell and Developmental Biology, 10, 815249.

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Immunofluorescence Analysis of Murine Kidney

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Mouse kidneys were collected at 28 days after ADR administration. Renal tissues were frozen with liquid nitrogen in OCT compound immediately after collection. Frozen tissues were sliced into 10-μm thickness using cryostat and attached onto glass slides. Sections were thawed with ice-cold acetone at − 20 °C for 5 min. After blocking with Protein Block Serum-Free (Dako, USA) at room temperature for 30 min, sections were reacted with primary antibody diluted at 1:100 and secondary antibody diluted at 1:300 with Antibody Diluent with Background Reducing Components (Dako, USA). Primary antibody was reacted overnight at 4 °C and secondary antibody was reacted for 1 h at room temperature. Polyclonal rabbit anti-WT-1 (sc-192; Santa Cruz Biotechnology), purified rat anti-mouse CD44 (#550538; BD, USA), Goat anti-rabbit IgG (H + L) Alexa Fluor 488 (A-11008; Thermo Scientific Inc., USA) and Goat anti-rat IgG (H + L) Alexa Fluor 488 (A-11006; Thermo scientific Inc., USA) were used for antibody reactions. Sections were incubated with 1 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) solution for 20 min at room temperature. Then, stained sections were mounted with VECTASHIELD mounting medium (Vector Laboratories, USA). Images were captured using BIOREVO microscope (Keyence, Japan).

Teramoto K., Tsurekawa Y., Suico M.A., Kaseda S., Omachi K., Yokota T., Kamura M., Piruzyan M., Kondo T., Shuto T., Araki E, & Kai H. (2020). Mild electrical stimulation with heat shock attenuates renal pathology in adriamycin-induced nephrotic syndrome mouse model. Scientific Reports, 10, 18719.

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Wnt/AMPK Signaling in Kidney Cells

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Kidney tissues or cells were lysed with RIPA buffer, and the supernatants were collected. Protein samples were separated using SDS-PAGE and transferred to polyvinylidene difluoride membranes. Membranes were blocked with 10% BSA for 1 h at room temperature and hybridized at 4°C overnight with specific antibodies: anti-Wnt1 (ab15251; abcam), anti-Wnt3a (SAB 2108434; Sigma), anti-β-catenin (610154; BD Biosciences, Frnklin Lakes, NJ), anti-α-tubulin (T9026; Sigma), anti-nephrin (20R-NP002; Fitzgerald), anti-WT1 (sc192; Santa Cruz Biotechnology), anti-AMPK (2532; CST), anti-p-AMPK (2535; CST). After incubation with the secondary antibody of the corresponding species origin, they were developed by the HRP-ECL luminescence method.

Yu Y., Mo H., Zhuo H., Yu C, & Liu Y. (2022). High Fat Diet Induces Kidney Injury via Stimulating Wnt/β-Catenin Signaling. Frontiers in Medicine, 9, 851618.

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Antibody and Reagent Sources for Immunoassays

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Goat polyclonal anti-CXCR3 (sc-9901), rabbit polyclonal anti-LHR (sc-25828) and anti-WT1 (sc-192) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Rabbit polyclonal anti-Caspase-3 (9662S) and mouse monoclonal anti-Caspase-8 (9746S) antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). Mouse monoclonal anti-MuV nucleoprotein (ab9876) and rabbit polyclonal anti-MVH (ab13840) antibodies were purchased from Abcam (Cambridge, UK). Mouse monoclonal anti-β-actin antibody (A5316) was purchased from Sigma (St. Louis, MO, USA). Horseradish-peroxidase (HRP)-conjugated secondary antibodies were purchased from Zhongshan Biotechnology Co. (Beijing, China). Recombinant mouse CXCL10 (250-16) and TNF-α (315-01A) were purchased from Peprotech (Rocky Hill, CT, USA). DEVD-fmk (264156), an inhibitor of caspase-3, was purchased from Calbiochem (La Jolla, CA, USA). Pomalidomide (S1567), an inhibitors of TNF-α, was purchased from Selleckchem (Houston, TX, USA). Annexin V-FITC apoptosis detection kit (FXP018) and ELISA kit for detecting mouse TNF-α (CME0004) were purchased from Beijing 4A Biotech Company (Beijing, China). ELISA kit for detecting mouse CXCL10 (BMS6018) was purchased from eBioscience (San Diego, CA, USA).

Jiang Q., Wang F., Shi L., Zhao X., Gong M., Liu W., Song C., Li Q., Chen Y., Wu H, & Han D. (2017). C-X-C motif chemokine ligand 10 produced by mouse Sertoli cells in response to mumps virus infection induces male germ cell apoptosis. Cell Death & Disease, 8(10), e3146-.

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