Hydrogenated soybean phospholipids (HSPC), cholesterol (Chol), and distearoyl phosphoethanolamine-PEG2000-Acrylamide (DSPE-PEG2000-ACA) were purchased from Shanghai Ponsure Biotech, Inc. Four-arm poly(ethylene glycol) amine (Tetra-PEG-amine) was provided by Xiamen Sinopeg company. Dibenzocyclooctyne-acid (DBCO acid, ≥95%) was supplied by Xi’an ruixi Biological Technology Co., Ltd. O-(7-azabenzotriazol-1-yl)-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU, 99%) and glutathione (GSH, 99%) were purchased from J&K Scientific Ltd. N,N-diisopropylethylamine (≥99%, DIEA), N,N’-methylenebisAcrylamide (99%, MBAA), N,N,N’,N’-tetramethylethylenediamine (99%, TEMED), and anhydrous N,N-dimethylformamide (99.8%, DMF) were provided by Sigma. Ammonium persulfate (99.99%, APS) and 1,11-diazido-3,6,9-trioxaundecane (≥95%, N3-PEG3-N3) were supplied by Aladdin. Acrylamide (99%, AM) was purchased from Adamas. Ethyl ether (≥99.7%, Et2O) and chloroform (≥99%, CHCl3) were provided by Sinopharm chemical reagent Co., Ltd. Ammonium sulfate (≥99%, (NH4)2SO4) and PBS buffer (10×, pH 7.4) were supplied by Sangon Biotech company. The PBS buffer used in all experiments was diluted ten times. Deionized water was used to prepare all aqueous solutions. Other reagents and solvents were used as received.
N n diisopropylethylamine
Manufactured by J&K Scientific
N,N-diisopropylethylamine is a colorless liquid organic compound used as a base and a catalyst in various chemical reactions. It is a tertiary amine with the chemical formula C8H19N.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)
Ethyl ether, by Sinopharm (1 mentions)
Ammonium sulfate, by Sangon (1 mentions)
N n n n tetramethylethylenediamine, by Merck Group (1 mentions)
O 7 azabenzotriazol 1 yl n n n n tetramethyluronium hexafluorophosphate hatu, by J&K Scientific (1 mentions)
Glutathione gsh, by J&K Scientific (1 mentions)
Acrylamide, by Adamas (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
N n methylenebisacrylamide, by Merck Group (1 mentions)
Chloroform, by Sinopharm (1 mentions)
Sodium cholate, by Merck Group (1 mentions)
Ms 222, by Merck Group (1 mentions)
N hydroxysuccinimide, by J&K Scientific (1 mentions)
Fm 1 43fx, by Thermo Fisher Scientific (1 mentions)
Mitotracker green fm, by Yeasen (1 mentions)
Dimethylformamide, by Sinopharm (1 mentions)
Methylene chloride, by Sinopharm (1 mentions)
Cupric sulfate, by Sinopharm (1 mentions)
Epichlorohydrin, by Dow (1 mentions)
6 protocols using n n diisopropylethylamine
1
Versatile Multifunctional Lipid Nanoparticles
2
Nanoparticle-Based Delivery of Therapeutic Peptide
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SS-31 peptide with a cysteine on the N-terminal was purchased from GL Biochem Ltd. (Shanghai, China). Poly(lactic-co-glycolic acid) (PLGA-COOH, 50:50, M.W. 13,000) and methoxy terminated PLGA-poly(ethylene glycol) (PLGA-mPEG2000, 50:50, M.W. 13,000) were purchased from Daigang Biotechnology Co, Ltd. (Jinan, China). PLGA-PEG conjugated rhodamine by amide linkage (PLGA13000-PEG3400-rhodamine) was purchased from Shanghai Ponsure Biotech, Inc. (Shanghai, China). Amino-poly(ethylene glycol)-maleimidyl (NH2-PEG3400-Mal) was purchased from JenKem Co., Ltd. (Beijing, China). Geranylgeranylacetone was purchased from Meilunbio (Dalian, China). Gentamicin sulfate (GT) was bought from DongKangYuan Science and Technology Ltd. (Wuhan, China). N-hydroxysuccinimide (NHS), dicyclohexylcarbodiimide (DCC), and N, N-Diisopropylethylamine (DIPEA) were obtained from J&K Scientific (Beijing, China). Sodium cholate, 2-[4-(dimethylamino)-styryl]-1-ethylpyridinium iodide (DASPEI), and MS-222 were obtained from Sigma-Aldrich (St. Louis, MO). Texas Red (TR) succinimidyl ester and FM1-43FX were both purchased from Molecular Probes, Inc. (Invitrogen, Eugene, OR). 3, 3-Diethyloxacarbocyanine iodide (DiOC2(3)) was bought from Tokyo Chemical Industry Development Co., Ltd. (Tokyo, Japan). MitoTracker® Green FM was obtained from YEASEN Biotechnology Co., Ltd. (Shanghai, China).
3
Synthesis and Characterization of PEG-based Antimicrobial
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Epichlorohydrin (Dow Chemical, USA), trifluoride diethyl etherate (BF3–OEt2, Tianjin Lianxing Biotechnology Co. Ltd.), 3-bromo-1-propyne (Aladdin, Shanghai), sodium ascorbate (VcNa, Aladdin, Shanghai), trypticase soy agar medium (TSA, AOBOX, Hengshui), trypticase soy liquid medium (TSB, AOBOX, Hengshui), 1,4-butylene glycol (BDO, KESHI, Chengdu), 4,4′-diphenylmethane diisocyanate (MDI, J&K, Beijing), tetrahydrofuran (THF, Bioway, Shanghai), anhydrous magnesium sulfate (MgSO4, Tianjin ZhiYuan Reagent Co. Ltd.) N,N-diisopropylethylamine (DIEA, J&K, Beijing) can be used directly. All other chemical reagents such as sodium hydride (NaH), dimethyl sulfoxide (DMSO), sodium azide (NaN3), methanol, sodium hydroxide (NaOH), diethyl ether, cupric sulfate (CuSO4), phosphate buffer (PBS), polypropylene glycol (PPG), methylene chloride (DCM) and dimethylformamide (DMF) were purchased from Sinopharm. S. aureus and E. coli were purchased from Guangdong Institute of Microbiology. mPEG with number average molecular weights of 5000, 3350 and 1000 (mPEG-5000, mPEG-3350 and mPEG-1000, Aladdin) were used without further purification.
4
Synthesis and Characterization of CTAB-Chitosan Nanoparticles
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Hexadecyltrimethylammonium bromide (CTAB, 99%), (3-aminopropyl)triethoxysilane (APTES, 98%), chitosan, superdry tetrahydrofuran (THF, 99.5%) and N,N-diisopropylethylamine (DIPEA, 99%) were purchased from J&K Chemical. Cyanuric chloride (CYC, 99%) was purchased from Energy Chemical. Bergamot oil and lemon oil were purchased from Shanghai Yuanye Bio-Technology Co.,Ltd. Ammonium hydroxide (AR, 25−28%), hydrogen chloride (HCl, AR, 36−38%), ethanol (AR), acetone (AR), dimethylsulfoxide (AR, DMSO), cyclohexane (AR), methanol (AR) and dichloromethane (DCM) were obtained from Sinopharm Chemical Reagent Co.,Ltd. CORT (97%) was purchased from TCI.
5
Synthesis and Characterization of Polymeric Nanomaterials
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1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDCI), triethylamine (TEA), 4-dimethylaminopyridine (DMAP), N,N-diisopropylethylamine (DIEA), N,N-dimethylacetamide (anhydrous, DMAc), dimethyl sulfoxide (anhydrous, DMSO), N,N-dimethylformamide (anhydrous, DMF), dichloromethane (anhydrous, DCM), 2,2′-azobis (2-methylpropionitrile) were purchased from J&K Scientific Ltd. (Shanghai, China). Nile Red (NiR), 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiL) and 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl indotricarbocyanineiodide (DiR) were all obtained from Life Technologies (Shanghai, China). NLG919 was purchased from Selleck Chemghai, China). PPa was purchased from Dibai Chem Tech Co., Ltd. (Shanghai, China). Triphosgene, 4-cyano-4-(dodecylsulfanylthiocarbonyl) sulfanylpentanoic acid (CTA) and bis(2-hydroxyethyl) disulfide were purchased from TCI (Shanghai, China). Enzyme-linked immunosorbent assay (ELISA) kits were ordered from Neobioscience Technology Co., Ltd. (Shenzhen, China). Kyn, Trp and 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) were ordered from Meilun Biotechnology Co., Ltd. (Dalian, China). The macromolecular chain transfer agent mPEG113-CTA and 2-(hexamethyleneimino) ethanol methacrylate (HMA) were synthenzied by following the similar procedure reported previously34 ,35 (link).
6
Intracellular Labeling and Organelle Imaging
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ICG were purchased from Aladdin Scientific; ICG-NHS and N,N-Diisopropylethylamine (DIPEA, 99 %) were purchased from J&K Scientific; BDA (D1956) were purchased from Molecular Probes; Colchicine was purchased from GlpBio; Anti S100β, anti NF200, anti β-Tubulin Polyclonal antibody were purchased from Proteintech; Dimethyl sulfoxide (DMSO, 99.9 %) and N,N-Dimethylformamide (DMF, 97 %) were purchased form LeYan; DMEM were purchased from Thermo Fisher; DAPI, Lyso-Tracker Green (C1047S) and Mito-Tracker Green (C1048) were purchased from Beyotime; ER-Tracker Green (BODIPY® FL Glibenclamide) was purchased from YEASEN Biotech; PC12 cells were purchased from the Chinese Academy of Sciences. The cells were cultivated in a DMEM medium in a humidified incubator with 5 % CO2 at 37 °C. All reagents were used as received without further purification. Deionized water was used in all experiments (18.2 MΩ cm−1). The 33-gauge 5 μL microsyringe was purchased from Hamilton.
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