Dox hcl

Dox HCl and Span20 were purchased from Sigma Aldrich^®, St. Louis, MO, USA. The synthesis of plier-like cationic lipid B (PCL-B) was kindly assisted by Ramkhamhaeng University and Mahasarakham University, Thailand. Cholesterol (Chol) was acquired from Carlo Erba Reagent (Milan, Italy). siAF488 was obtained from Qiagen (Santa Clarita, CA, USA). siRNA targeting apoptosis-related proteins including Mcl-1, Bcl-2, and survivin was obtained from Ambion™ Silencer™ siRNA, Thermo Fisher Scientific (Waltham, MA, USA). Lipo2k and siNT were purchased from Invitrogen (Carlsbad, CA, USA).

Poly (ε-caprolactone) (PCL, Mw = 70,000–90,000) was obtained from Sigma–Aldrich (USA). 2,2,2-Trifluoroethanol (TFE) was purchased from Alfa Aesar, USA. DOX.HCl was provided by Sigma, Korea. Dopamine hydrochloride was obtained from Sigma (Korea). Live and the Dead cell assay kit were purchased from Sigma, Korea. All other chemicals used were of analytical grade and were used as received.

ML (purity > 98%) was isolated from Costus speciosus rhizomes extract (Supplementary Materials). DOX HCl was obtained from Sigma-Aldrich (St. Louis, MO, USA). Remaining chemicals conformed with the highest available commercial purity.

The lactulose was provided by Sedico company as a free gift (Sedico, Egypt). From Sigma-Aldrich, we purchased copper sulphate pentahydrate CuSO₄.5H₂O with a molecular mass of 249.69 g/mol and ferrous sulphate heptahydrate FeSO₄.7H₂O with a molecular mass of 278.01 g/mol. Both of these chemicals are sulphates (St Louis, MO, USA). Polyethylene glycols 6000, was obtained from Merck, Germany. As an adsorbate, DOX HCL was purchased from Sigma-Aldrich.

LOX-1 KO mice with a C57/BL6J background were produced as described previously [20 (link)] and LOX-1 KO mice and control wild-type (WT) C57/BL6J strain mice were a kind gift from Dr. Tatsuya Sawamura (National Cerebral and Cardiovascular Center, Suita, Japan/ Department of Physiology, Shinshu University School of Medicine, Matsumoto, Japan). Male LOX-1 KO mice and WT mice were used at the age of 12 weeks. Mice were housed in a facility with a 12-hour/12-hour light/dark cycle and were given free access to water and standard rodent chow. The room was kept specific-pathogen-free. DOX HCl (Sigma Chemical Co.) was dissolved in saline and administered by intraperitoneal injection at a dose of 20 mg/kg. This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (NIH Publication, 8th Edition, 2011). The protocol was approved by the Institutional Animal Research Committee of Gifu University (Permit Number: 17−68).

STS and DOX HCl were purchased from Sigma (St. Louis, MO, USA). All the chemicals used in the preparation of the physiologic salt solution were purchased from Merck (Darmstadt, Germany) (NaCl, MgCl₂, KCl, NaH₂PO₄, NaHCO₃, glucose, CaCl₂, KH₂PO₄, and EDTA).