UC-MSCs were transferred into 15-ml centrifuge tubes (Corning Incorporated) at 5×105 cells/tube and centrifuged at 110 × g for 3 min at room temperature. The pelleted micromass of UC-MSCs formed at the bottom of each tube was treated with chondrogenic medium for three weeks. Chondrogenic medium comprised high-glucose (HG)-DMEM (Gibco; Thermo Fisher Scientific, Inc.), 0.1 µM dexamethasone, 50 µg/ml AsA, 100 µg/ml sodium pyruvate (Lonza Group, Basel, Switzerland), 40 µg/ml proline (Sigma-Aldrich; Merck Millipore), 10 ng/ml TGF-β1 (PeproTech, Inc.) and 1% insulin-transferrin-selenium-A (Gibco; Thermo Fisher Scientific, Inc.).
15 ml centrifuge tube
Manufactured by Corning
Sourced in United States
The 15 mL centrifuge tube is a laboratory equipment designed to hold and contain samples during centrifugation. It has a volume capacity of 15 milliliters and is made of durable, high-quality materials.
Automatically generated - may contain errors
Lab products found in correlation
Interleukin 2 (il 2), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Trypsin, by Thermo Fisher Scientific (2 mentions)
Percoll, by Corning (2 mentions)
Percoll, by GE Healthcare (2 mentions)
Proline, by Merck Group (1 mentions)
Insulin transferrin selenium a, by Thermo Fisher Scientific (1 mentions)
Tgf β1, by Thermo Fisher Scientific (1 mentions)
Dmem hg, by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Lonza (1 mentions)
Nexion 350, by PerkinElmer (1 mentions)
Dynabeads human t expander cd3 cd28, by Thermo Fisher Scientific (1 mentions)
Interleukin 7 (il 7), by Thermo Fisher Scientific (1 mentions)
Il 15, by Thermo Fisher Scientific (1 mentions)
Lymphoprep, by Takeda Pharmaceuticals (1 mentions)
Rpmi 1640 medium, by Corning (1 mentions)
Agilent 7900, by Agilent Technologies (1 mentions)
Dmem f12 medium, by Corning (1 mentions)
Collagenase type 1, by Solarbio (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
28 protocols using 15 ml centrifuge tube
1
Chondrogenic Differentiation of UC-MSCs
2
DATS Concentration Preparation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The molecular weight and purity of DATS were 178.34 and 99.2%, respectively. The DATS (50 µL) was weighed to calculate the mother liquor concentration, which was 6.27 mol/L. DMSO was used to dilute the DATS concentration to 10, 20, 40, 80, 160 µM in 1.5 mL centrifuge tubes (Corning, USA); the prepared concentrations were stored in a refrigerator (Siemens AG, Germany) at -20°C, protected away from light.
3
Selenium Quantification in Plasma and Whole Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma samples were diluted with 0.5% (v/v) HNO3 (1:20). Whole bloods were diluted with 0.5% (v/v) HNO3 and 0.05% (v/v) Triton-X-100 (1:25). In order to avoid contamination of samples, the experiment was carried out on a clean bench. Trace elements dissolution was avoided with 15 mL centrifuge tubes (Corning, NY, USA). Se concentration in the plasma and whole blood was measured by inductively coupled plasma mass spectrometry (ICP-MS, PerkinElmer, NexION 350, Waltham, MA, USA). The plasma quality-control samples (Clincheck, serum Level-2, Munich, Germany; Seronorm, serum Level-2, Billingstad, Norway) were used to monitor and analyze at intervals of 20 samples to ensure the precision and accuracy of detection. The whole-blood quality-control samples (Clincheck, whole blood Level-2, Munich, Germany; Seronorm, whole blood Level-2, Billingstad, Norway) were used to monitor and analyze at intervals of 10 samples.
The rate of recovery of Se was 105.87% and 110.53% in plasma and whole blood, respectively. The inter-day precision of Se for plasma and whole blood was 4.86% and 6.52%, while the intra-day precision values were 5.83% and 8.01%. The limit of detection (LOD) was 0.38 μg/L and 0.75 μg/L for plasma and whole-blood Se. The limit of quantitation (LOQ) was 1.26 μg/L and 2.51 μg/L for plasma and whole-blood Se.
The rate of recovery of Se was 105.87% and 110.53% in plasma and whole blood, respectively. The inter-day precision of Se for plasma and whole blood was 4.86% and 6.52%, while the intra-day precision values were 5.83% and 8.01%. The limit of detection (LOD) was 0.38 μg/L and 0.75 μg/L for plasma and whole-blood Se. The limit of quantitation (LOQ) was 1.26 μg/L and 2.51 μg/L for plasma and whole-blood Se.
4
Isolation and Activation of Human T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples obtained from healthy donors, aged 20–40 years, recruited from the Southeast University, signed written consent was obtained before the donation of peripheral blood. The blood samples were diluted with PBS plus 2% (v/v) FBS and added to the upper layer of Lymphoprep (Nycomed, Oslo, Norway) before centrifugation at 1006.2 × g for 30 min. The mononuclear cell layer at the interface between serum and the separation reagent was collected into 15-mL centrifuge tubes (Corning Incorporated, Corning, NY, USA) and washed with the medium. Furthermore, the cells were counted and maintained in RPMI 1640 medium (Corning Incorporated) in 1.5-mL centrifuge tubes (Corning Incorporated). DynabeadsTM Human T-Expander CD3/CD28 (Cat: 11141D, Gibco, Gaithersburg, MD, USA) was added at a 3:1 bead:T cell ratio at 37 °C in 5% CO2 for 1 h. After incubation, the tubes were inserted into the magnetic separation rack at RT for 10 min. The absorbed T cells were resuspended in complete medium supplemented with 10% (v/v) FBS, 100 U/mL penicillin, 100 μg/mL streptomycin, 300 U/mL IL-2 (PeproTech, Rocky Hill, NJ, USA), 10 ng/mL IL-7 (PeproTech), and 5 ng/mL IL-15 (PeproTech).
5
Chorionic and Dermal Tissue Collection for Prenatal Screening
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All samples were obtained under Institutional Review Board approved protocols with informed consent from all participants for research use at Nanfang Hospital, Southern Medical University (approval no. NFEC-2017-050). In total, 437 SM samples within 20 weeks of gestation with array CGH results, and 964 SM samples within 20 weeks of gestation but without array CGH results, were collected between August 2017 and February 2018. The maternal age range was 18–47 years, with a mean of 30 years. Gestational age ranged from 5 to 20 weeks, with a mean of 9 weeks and 2 days. Following collection, SM samples (chorionic or dermal tissue of SM) were rinsed three times in PBS and then stored in 15 ml centrifuge tubes (Corning Inc.) at −20°C until use.
6
Quantifying Total Arsenic Bioaccumulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The total concentration of As was analyzed as depicted in an earlier study [6 (link)]. The tissues and diets of mice were placed in a freeze-drying machine for 48 h till a constant weight was achieved and then ground into powder to ensure homogenization. About 0.1–0.3 g of samples was placed in 15 mL centrifuge tubes (Corning, Guangzhou, USA), followed by 1 mL HNO3. Before placing the samples in a metal bath (MK200-4, Allsheng, China), the tubes were incubated at 25–26 °C for 1 h, allowed to react at 80 °C for 24 h until clarification, and then diluted for detection.
The total As content was detected using inductively coupled plasma mass spectrometry (ICP-MS, Agilent 7900, Japan). Total As bioaccumulation (μg g−1) = total As concentrations detected by ICP-MS (μg L−1) × solution volume (L)/sample weight (g). Tuna fish standard reference material (SRM) (BCR-627, Institute for Reference Materials and Measurements, Geel, Belgium) was analyzed to evaluate the accuracy of our digestion method. The SRM contained a total As the content of 4.7 ± 0.8 μg g−1 (97.9% recovery of 4.8 ± 0.3 μg g−1 certified value, n = 6).
The total As content was detected using inductively coupled plasma mass spectrometry (ICP-MS, Agilent 7900, Japan). Total As bioaccumulation (μg g−1) = total As concentrations detected by ICP-MS (μg L−1) × solution volume (L)/sample weight (g). Tuna fish standard reference material (SRM) (BCR-627, Institute for Reference Materials and Measurements, Geel, Belgium) was analyzed to evaluate the accuracy of our digestion method. The SRM contained a total As the content of 4.7 ± 0.8 μg g−1 (97.9% recovery of 4.8 ± 0.3 μg g−1 certified value, n = 6).
7
Isolation and Culture of Mouse Adipose-Derived Mesenchymal Stem Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fat was isolated from the groins of 4-week-old C57BL/6 J mice on a clean bench and transferred to 15 mL centrifuge tubes (Corning, New York, USA) containing 5 mL of DMEM/F12 medium (Gibco, MA, USA). Collagenase type I (1 mg/mL) (Solarbio Life Science, Beijing, China) was added and incubated at 37 °C for 1.5 h under shock conditions (200 rpm). Fully digested adipose tissue was filtered through a 40-μm mesh strainer and centrifuged at 1500 rpm for 5 min to obtain cells, and this process was repeated once. The cells were resuspended in DMEM/F12 medium (Corning, New York, USA) containing 1% antibiotic cocktail (Solarbio Life Sciences, Beijing, China) and 15% fetal bovine serum, seeded on 6 cm dishes (Corning, New York, USA) and incubated in a 37 °C incubator with 5% CO2. The medium was changed every 48 h. The isolated MSCs were used in subsequent experiments.
8
Exosome Isolation from Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells growing in the logarithmic phase were digested using 1 mL of 0.25% pancreatic enzymes; digestion was terminated using 2 mL of complete medium. Cells were transferred into a 15 mL centrifuge tube (Corning, NY, United States, #430790) and centrifuged for 3 min at 1500 rpm. Cell numbers were counted using a cell counting plate. Cells were seeded in a 100 mm culture dish (Corning #430167) at a density of 80% after cell apposition and incubated at 37°C overnight in an atmosphere of 5% CO2. Following cell apposition, the culture medium was replaced with a medium containing 10% exosome-free serum and subsequently further incubated for 48 h. The supernatant was collected in a 50 mL centrifuge tube (Corning #430828) and centrifuged at 300 × g for 10 min, followed by 2000 × g for 20 min to remove the cells, and further at l0000 × g for 30 min to remove the subcellular component. A final centrifugation was performed at l0000 × g for 60 min to obtain the exosomes. The obtained exosomes were suspended in 30 mL phosphate-buffered saline (PBS) solution, followed by centrifugation at 10,0000 × g 60 min. The purified exosomes were suspended in 1 mL PBS solution and stored at 4°C for further experiments.
9
Isolation and Establishment of Liver Cancer Cell Line
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A portion of tumor tissue (3 gm) resected from liver during the operation and immediately washed in DMEM medium. The tissue was cut 1 mm3 into tissue-culture flasks (Corning Glass Works, Corning, NY), washed twice with l × PBS (Ca2+, Mg2+ free) and 1 × Wash Medium (Invitrogen), After digestion using 0.1% Collagenase Type IV (Gibco) in DMEM for 30 min at 37 °C and shaked occasionally in a 15 ml centrifuge tube (Corning), the suspension was filtered by 45 μm cell strainer to remove large fragments and the collected liquid was centrifuged consecutively at 1500 rpm, 1000 rpm, 800 rpm and 600 rpm for 5 min, respectively. Cancer cells were resuspended using primary culture medium (DMEM/F12 + 10% FBS + 1% penicillin–streptomycin + 0.2 U/ml insulin) and transferred to a tissue-culture flask overnight in a humidified incubator at 37 °C with 5% CO2. Fresh medium can be changed every day.
In the first 3 months, fibroblast cells grew fast. During this time, some tumor cell “island” emerged. We removed the fibroblast scratching by pipetting tips and the tumor cell “island” became bigger. Tumor cell clones were picked out from the primary culture and purified, at passage 40 (13 months after we picked out the tumor cell clone), a stable cell line (HCS1220) was considered to have been established.
In the first 3 months, fibroblast cells grew fast. During this time, some tumor cell “island” emerged. We removed the fibroblast scratching by pipetting tips and the tumor cell “island” became bigger. Tumor cell clones were picked out from the primary culture and purified, at passage 40 (13 months after we picked out the tumor cell clone), a stable cell line (HCS1220) was considered to have been established.
10
Analyzing Sonication's Impact on Arsenopyrite
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The effectiveness of sonication in removing fines was analyzed by measuring the average diameter of the particles by laser diffraction at Particle Tech Labs (Illinois, USA). Ten grams of sonicated and dried arsenopyrite were shipped in a 15 mL centrifuge tube (Corning USA) for analysis. The particle diameter distribution was calculated on a % volume basis.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!