Aav8 hsyn dio gq mcherry

Manufactured by Addgene

AAV8-hSyn-DIO-Gq–mCherry is a recombinant adeno-associated virus (rAAV) vector that expresses a Gq-coupled Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) fused to the fluorescent protein mCherry under the control of the human synapsin (hSyn) promoter. This vector can be used for Cre-dependent expression of the Gq-DREADD in target cell populations.

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Lab products found in correlation

C fos antibody, by Cell Signaling Technology (1 mentions) Aav8 hsyn mcherry, by Addgene (1 mentions)

Spelling variants of this product

AAV8-hSyn-DIO-mCherry (26 citations) AAV8-hSyn-DIO-hM3D(Gq)-mCherry (10 citations) AAV8-hSyn-mCherry (8 citations)

2 protocols using aav8 hsyn dio gq mcherry

Targeted Optogenetic and Chemogenetic Manipulation

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AAV8-hSyn-DIO-Gq–mCherry (Addgene, no.44361-AAV8), AAV8-hSyn-DIO-Gi–mCherry (Addgene, no. 44362-AAV8), AAV8-hSyn-mCherry (Addgene, no. 114472-AAV8) and AAV9-Syn-GCaMP6m-WPRE-SV40 (Addgene, no. 100841-AAV9) were obtained from Addgene.
AAV5-ESARE-ER-Cre-ER-PEST was obtained from UNC, and AAV8-Ef1a-DIO hChR2(H134R)-p2AScarlett and AAV8-CaMKIIa-hChR2-p2A-oScarlet were obtained from Stanford. c-Fos antibody was purchased commercially (Cell signaling, no. 2250) as was CNO (Hello, no. HB1807).

Lal N.K., Le P., Aggarwal S., Zhang A., Wang K., Qi T., Pang Z., Yang D., Nudell V., Yeo G.W., Banks A.S, & Ye L. (2023). Xiphoid nucleus of the midline thalamus controls cold-induced food seeking. Nature, 621(7977), 138-145.

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Viral Transduction of Genetically Encoded Sensors

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AAV8-hSyn-DIO-Gq-mCherry (Addgene # 44361-AAV8) and
AAV1-Syn-Flex-GCaMP6s-WPRE-SV40 (Addgene # 100845-AAV1) were obtained from
Addgene. AAV2/1-hSyn-Flex-TeLC-EYFP^{56 (link)} was prepared through Boston Children’s Hospital
Viral Core. All viruses were diluted with PBS to a final concentration of 5E12
– 1E13 genome copies per mL before stereotaxic delivery into the mice
brain.

Hrvatin S., Sun S., Wilcox O.F., Yao H., Lavin-Peter A.J., Cicconet M., Assad E.G., Palmer M.E., Aronson S., Banks A.S., Griffith E.C, & Greenberg M.E. (2020). Neurons that regulate mouse torpor. Nature, 583(7814), 115-121.

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