Log In Sign up
The largest database of trusted experimental protocols

1 fold protein thermal shift dye

Manufactured by Thermo Fisher Scientific
Visit Supplier

The 1-fold protein thermal shift dye is a laboratory reagent used to assess the thermal stability of proteins. It binds to the hydrophobic regions of proteins that are exposed during thermal denaturation, causing a shift in the protein's melting temperature. This information can be used to evaluate the structural integrity and stability of proteins under different experimental conditions.

Automatically generated - may contain errors

Lab products found in correlation

Protein thermal shift software, by Thermo Fisher Scientific (4 mentions)

Spelling variants of this product

Protein Thermal Shift Dye (61 citations) Thermal shift dye (3 citations)

4 protocols using 1 fold protein thermal shift dye

1

NLRP3 and NEK7 Thermal Stability

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified 2 μM NLRP3 or NEK7 was mixed with 1-fold protein thermal shift dye (Thermo fisher scientific) and 200 μM MCC950 or ADP or 100 μM MCC950 plus 100 μM ADP in a 20 μl reaction volume. Thermal scanning (25 to 75 °C at 1.5 °C/min) was performed and melting curves were recorded on a StepOne RT-PCR machine. Data analysis was done by Protein Thermal Shift™ Software (Thermo Fisher Scientific).
Sharif H., Wang L., Wang W.L., Magupalli V.G., Andreeva L., Qiao Q., Hauenstein A.V., Wu Z., Nunez G., Mao Y, & Wu H. (2019). Structural mechanism for NEK7-licensed NLRP3 inflammasome activation. Nature, 570(7761), 338-343.
+ Open protocol
+ Expand
2

Thermal Stability Profiling of BCL10 Mutants

Check if the same lab product or an alternative is used in the 5 most similar protocols
BCL10 full-length, R58Q, and E140X filaments purified from the void peak of Superdex200 were mixed with 1-fold protein thermal shift dye (Thermo Fisher Scientific). Thermal scanning (25°C to 95°C at 1°C/minute) was performed, and melting curves were recorded on a StepOne RT-PCR machine. Data analysis was done by Protein Thermal Shift Software (Thermo Fisher Scientific).
Xia M., David L., Teater M., Gutierrez J., Wang X., Meydan C., Lytle A., Slack G.W., Scott D.W., Morin R.D., Onder O., Elenitoba-Johnson K.S., Zamponi N., Cerchietti L., Lu T., Philippar U., Fontan L., Wu H, & Melnick A.M. (2022). BCL10 Mutations Define Distinct Dependencies Guiding Precision Therapy for DLBCL. Cancer Discovery, 12(8), 1922-1941.
+ Open protocol
+ Expand
3

Thermal Stability Analysis of BCL10 Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified BCL10 FL, R58Q and E140X filaments purified from the void peak of Superdex200 were mixed with 1-fold protein thermal shift dye (Thermo Fisher Scientific). Thermal scanning (25 to 95 °C at 1 °C/min) was performed and melting curves were recorded on a StepOne RT-PCR machine. Data analysis was done by Protein Thermal Shift™ Software (Thermo Fisher Scientific).
Xia M., David L., Teater M., Gutierrez J., Wang X., Meydan C., Lytle A., Slack G.W., Scott D.W., Morin R.D., Onder O., Elenitoba-Johnson K.S., Zamponi N., Cerchietti L., Lu T., Philippar U., Fontan L., Wu H, & Melnick A.M. (2022). BCL10 Mutations Define Distinct Dependencies Guiding Precision Therapy for DLBCL. Cancer Discovery, 12(8), 1922-1941.
+ Open protocol
+ Expand
4

NLRP3 and NEK7 Thermal Stability

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified 2 μM NLRP3 or NEK7 was mixed with 1-fold protein thermal shift dye (Thermo fisher scientific) and 200 μM MCC950 or ADP or 100 μM MCC950 plus 100 μM ADP in a 20 μl reaction volume. Thermal scanning (25 to 75 °C at 1.5 °C/min) was performed and melting curves were recorded on a StepOne RT-PCR machine. Data analysis was done by Protein Thermal Shift™ Software (Thermo Fisher Scientific).
Sharif H., Wang L., Wang W.L., Magupalli V.G., Andreeva L., Qiao Q., Hauenstein A.V., Wu Z., Nunez G., Mao Y, & Wu H. (2019). Structural mechanism for NEK7-licensed NLRP3 inflammasome activation. Nature, 570(7761), 338-343.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!