FastDigest restriction enzymes, pfu DNA polymerase, T4 DNA ligase, and media components were purchased from Thermo Scientific (Waltham, MA, USA). pEASY-Blunt Simple Cloning Kit was purchased from TransGen Biotech (Beijing, China). Plasmid miniprep kit, gel extraction kit, and PCR purification kit were purchased from Omega Bio-tek (Norcross, GA, USA). Gene sequencing and oligonucleotide synthesis were performed by Tsingke Biological Technology (Beijing, China). Isobutyraldehyde standard was purchased from Acros Organics (Geel, Belgium). All the other reagents were purchased from Sigma–Aldrich (St. Louis, Mo, USA).
Media components
Manufactured by Thermo Fisher Scientific
Sourced in United States
Media components are essential laboratory supplies used in the preparation of various culture media for microbiology, cell culture, and biotechnology applications. They provide the necessary nutrients, growth factors, and other components required for the cultivation and growth of microorganisms, cells, and tissues in controlled laboratory settings.
Automatically generated - may contain errors
Lab products found in correlation
T4 dna ligase, by Thermo Fisher Scientific (1 mentions)
Fastdigest restriction enzyme, by Thermo Fisher Scientific (1 mentions)
Gel extraction kit, by Omega Bio-Tek (1 mentions)
Pcr purification kit, by Omega Bio-Tek (1 mentions)
Pfu dna polymerase, by Thermo Fisher Scientific (1 mentions)
Peasy blunt simple cloning kit, by Transgene (1 mentions)
Plasmid miniprep kit, by Omega Bio-Tek (1 mentions)
Dna extraction kit, by Qiagen (1 mentions)
Dna polymerase, by Thermo Fisher Scientific (1 mentions)
Digestive enzymes, by Merck Group (1 mentions)
2 protocols using media components
1
Optimized Genetic Transformation Protocol
2
Comprehensive Biomolecular Sourcing Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Digestive enzymes and buffer components were purchased through Sigma-Aldrich. Media components, HPLC consumables and DNA-polymerase were purchased through Thermo Fisher Scientific (Waltham, MA, United States). DNA extraction kits were purchased from Qiagen (Germantown, MD, United States). Sequencing supplies were from Illumina (San Diego, CA, United States).
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