Log In Sign up
The largest database of trusted experimental protocols

Caspase glo 3 7 luminescence assay

Manufactured by Promega
Visit Supplier
Sourced in United States

The Caspase-Glo 3/7 luminescence assay is a reagent system that measures the activity of caspase-3 and caspase-7, two key enzymes involved in the apoptotic pathway. The assay utilizes a luminogenic caspase-3/7 substrate, which upon cleavage by the enzymes, generates a luminescent signal proportional to the amount of caspase activity present in the sample.

Automatically generated - may contain errors

Lab products found in correlation

Cell death detection elisaplus kit, by Roche (3 mentions) Microplate reader, by Agilent Technologies (2 mentions) Etoposide, by Merck Group (2 mentions) Staurosporine, by Merck Group (2 mentions) Fitc annexin 5 dead cell apoptosis kit, by Thermo Fisher Scientific (2 mentions) Turner biosystems luminometer, by Promega (2 mentions) Passive lysis buffer (plb), by Promega (2 mentions) Cucl2, by Thermo Fisher Scientific (1 mentions) Alamarblue, by Bio-Rad (1 mentions) Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (1 mentions) Micro bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Eclipse te2000 u, by Nikon (1 mentions) Sa β galactosidase staining kit, by Cell Signaling Technology (1 mentions) Synergy ht microplate reader, by Agilent Technologies (1 mentions) 96 well plate, by Thermo Fisher Scientific (1 mentions) Ldh assay kit, by Merck Group (1 mentions) Sa β gal, by Cell Signaling Technology (1 mentions) Fitc annexin 5 apoptosis detection kit, by BD (1 mentions) Wallac 1420 apparatus, by PerkinElmer (1 mentions) Celltitreglo luminescence assay, by Promega (1 mentions)

20 protocols using caspase glo 3 7 luminescence assay

1

Measuring Apoptosis Markers in Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
We measured caspase 3 and 7 activity as indicators of apoptosis. 3T3 cells were nucleofected with pControl or pFlag-UCKL-1 and incubated for 16 hours at 37°C. Staurosporine (12.5 nM) was then added for an additional 6 hours. Caspase activity and cell counts were assessed 22 hours after nucleofection. K562 cells were nucleofected with pControl or pFlag-UCKL-1 and incubated for 16 hours at 37°C. Staurosporine (2.5 μM) or etoposide (50 μM) (Sigma, St. Louis, MO) was then added for an additional 8 hours. Caspase activity was measured 24 hours after nucleofection. Activity was assessed using the Caspase-Glo 3/7 luminescence assay (Promega, Madison, WI), following the manufacturer’s instructions. Triplicate samples were analyzed for luminescence using a microplate reader (Bio-Tek, Winooski, VT). Caspase activity in UCKL-1 transfected cells was calculated relative to control transfected cells.
Gullickson G., Ambrose E.C., Hoover R.G, & Kornbluth J. (2016). Uridine Cytidine Kinase Like-1 Enhances Tumor Cell Proliferation and Mediates Protection from Natural Killer-Mediated Killing. International journal of immunology and immunotherapy, 3(1), 10.23937/2378-3672/1410018.
+ Open protocol
+ Expand
2

Apoptosis Quantification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Caspase-3/7 activation was measured using the Caspase-Glo 3/7 Luminescence assay (Promega), according to the manufacturer's instructions. Annexin V was measured using a FITC Annexin V/Dead Cell Apoptosis Kit (ThermoFisher). The flow cytometry data were analyzed using FlowJo (v10.2).
Goss K.L., Koppenhafer S.L., Harmoney K.M., Terry W.W, & Gordon D.J. (2017). Inhibition of CHK1 sensitizes Ewing sarcoma cells to the ribonucleotide reductase inhibitor gemcitabine. Oncotarget, 8(50), 87016-87032.
+ Open protocol
+ Expand
3

Caspase-3/7 Activity in Kidney Apoptosis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Apoptosis was assessed in whole kidney lysates using the Caspase-Glo 3/7 luminescence assay (Promega) measuring levels of active-caspase 3/7, as previously described(29 (link)). Luminescence was measured using a Turner Biosystems luminometer (Promega). Experiments were performed in 10 independent kidney samples each, from RCALC2 mutant mice and WT littermates. Data was expressed as fold change±SEM for each group. Statistical analyses were performed using two-way ANOVA.
Gorvin C.M., Ahmad B.N., Stechman M.J., Loh N.Y., Hough T.A., Leo P., Marshall M., Sethi S., Bentley L., Piret S.E., Reed A., Jeyabalan J., Christie P.T., Wells S., Simon M.M., Mallon A., Schulz H., Huebner N., Brown M.A., Cox R.D., Brown S.D, & Thakker R.V. (2018). An N‐Ethyl‐N‐Nitrosourea (ENU)‐Induced Tyr265Stop Mutation of the DNA Polymerase Accessory Subunit Gamma 2 (Polg2) Is Associated With Renal Calcification in Mice. Journal of Bone and Mineral Research, 34(3), 497-507.
+ Open protocol
+ Expand
4

GHK Peptide Effects on MSC Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
MSC were seeded at 2.5×105 cells/cm2 in multi-well plates and allowed to adhere overnight. Media was refreshed with media supplemented with GHK peptide (G4GHKSP, Commonwealth Biotechnologies) at 1, 10, 100, or 500 ng/mL, together with ammonium tetrathiomolybdate (50 μg/mL, Acros Organics) to deplete endogenous copper from serum [26 (link), 28 (link)]. Unless otherwise stated, all experiments were performed in the presence of CuCl2 (40 μM, Fisher Scientific) [29 ] to provide a more consistent concentration of copper than achievable with FBS. MSC were cultured for 3 days, and metabolic activity was measured using alamarBlue (AbD Serotec) according to the manufacturer’s instruction. Cells were collected in 1X passive lysis buffer (Promega), and protein concentration and DNA content were determined using the Micro BCA protein assay kit (Thermo Scientific) and Quant-iT Pico Green dsDNA assay kit (Invitrogen), respectively. Cell morphology was examined by brightfield microscopy on day 3 using a Nikon Eclipse TE2000-U and SpotRT digital camera. To determine apoptosis in GHK-exposed cells, MSC were collected in passive lysis buffer after incubation with GHK for 3 days, and caspase activity was quantified using the Caspase-Glo 3/7 luminescence assay (Promega). Luminescence was measured on a multifunctional plate reader (Synergy HTTR) and normalized to DNA content within each well [30 ].
Jose S., Hughbanks M.L., Binder B.Y., Ingavle G.C, & Leach J.K. (2014). Enhanced trophic factor secretion by mesenchymal stem/stromal cells with Glycine-Histidine-Lysine (GHK)-modified alginate hydrogels. Acta biomaterialia, 10(5), 1955-1964.
+ Open protocol
+ Expand
5

Apoptosis Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Caspase-3/7 activation was measured using the Caspase-Glo 3/7 Luminescence assay (Promega), according to the manufacturer's instructions. Annexin V was measured using a FITC Annexin V/Dead Cell Apoptosis Kit (ThermoFisher). The flow cytometry data were analyzed using FlowJo.
Goss K.L, & Gordon D.J. (2016). Gene expression signature based screening identifies ribonucleotide reductase as a candidate therapeutic target in Ewing sarcoma. Oncotarget, 7(39), 63003-63019.
+ Open protocol
+ Expand
6

Amylin Oligomers Induce Caspase 3/7 Activity

Check if the same lab product or an alternative is used in the 5 most similar protocols
Caspase 3/7 activity in HBVP after 3, 6, 12, 18 and 24 h exposure to oligomer-EP, fibril-EP amylin or their control was analysed using Caspase-Glo 3/7 luminescence assay according to the manufacturer’s instructions (Promega, Fitchburg, WI). The cell experiment was performed in triplicate at each time point.
Schultz N., Byman E., Fex M, & Wennström M. (2016). Amylin alters human brain pericyte viability and NG2 expression. Journal of Cerebral Blood Flow & Metabolism, 37(4), 1470-1482.
+ Open protocol
+ Expand
7

Caspase-3/7 Activity Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Caspase-3/7 activation was measured using the Caspase-Glo 3/7 Luminescence Assay (Promega Corp. Madison, Wisc., USA) according to the manufacturer’s instructions. In a 6-well plate 3×105 cells were incubated. The day after, the cells were treated with siRNAs or PDGFRB inhibitors, for 72 h. Then, the cells were collected by trypsinization, and approximately 15×103 cells were transferred in a 96-well white plate. Caspase-3/7-Glo reagent was added, and the samples were incubated at 37°C for 1 h. The luminescence that is proportional to the caspase 3/7 activities was determined by luminometer (Tecan Sunrise, Austria GMBH).
Melaiu O., Catalano C., De Santi C., Cipollini M., Figlioli G., Pellè L., Barone E., Evangelista M., Guazzelli A., Boldrini L., Sensi E., Bonotti A., Foddis R., Cristaudo A., Mutti L., Fontanini G., Gemignani F, & Landi S. (2017). Inhibition of the platelet-derived growth factor receptor beta (PDGFRB) using gene silencing, crenolanib besylate, or imatinib mesylate hampers the malignant phenotype of mesothelioma cell lines. Genes & Cancer, 8(1-2), 438-452.
+ Open protocol
+ Expand
8

Quantifying Cell Death Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA fragmentation and caspase‐3/7 activation were assayed using the Cell Death Detection ELISAPLUS kit (Roche, USA) and the Caspase‐Glo 3/7 Luminescence Assay (Promega Corp, Madison, WI), respectively, according to the manufacturer's instructions.
Maille E., Levallet J., Dubois F., Antoine M., Danel C., Creveuil C., Mazieres J., Margery J., Greillier L., Gounant V., Moro‐Sibilot D., Molinier O., Léna H., Monnet I., Bergot E., Langlais A., Morin F., Scherpereel A., Zalcman G, & Levallet G. (2022). A defect of amphiregulin release predicted longer survival independently of YAP expression in patients with pleural mesothelioma in the IFCT‐0701 MAPS phase 3 trial. International Journal of Cancer, 150(11), 1889-1904.
+ Open protocol
+ Expand
9

Apoptosis and Senescence Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
Apoptosis was quantified using Caspase-Glo 3/7 luminescence assay (Promega, G8091). Senescence staining was carried out using SA-β-Galactosidase Staining Kit (Cell Signalling Technologies, 9680) and quantified using Galacto-Light Plus Beta-Galactosidase Reporter Gene Assay System (Invitrogen, T1007) following the manufacturer’s protocol, except modified to use lysosomal reaction buffer (100 mM sodium phosphate pH 6, 20 μM MgCl2).
Lowe D.J., Herzog M., Mosler T., Cohen H., Felton S., Beli P., Raj K., Galanty Y, & Jackson S.P. (2020). Chronic irradiation of human cells reduces histone levels and deregulates gene expression. Scientific Reports, 10, 2200.
+ Open protocol
+ Expand
10

Apoptosis Assessment via ELISA and Luminescence

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA fragmentation and Caspase 3/7 activation were assayed using the Cell Death Detection ELISA plus kit (Roche, USA) and the Caspase-Glo 3/7 Luminescence Assay (Promega Corp. Madison, WI, USA), respectively, according to the manufacturer’s instructions.
Maille E., Brosseau S., Hanoux V., Creveuil C., Danel C., Bergot E., Scherpereel A., Mazières J., Margery J., Greillier L., Audigier-Valette C., Moro-Sibilot D., Molinier O., Corre R., Monnet I., Gounant V., Langlais A., Morin F., Levallet G, & Zalcman G. (2019). MST1/Hippo promoter gene methylation predicts poor survival in patients with malignant pleural mesothelioma in the IFCT-GFPC-0701 MAPS Phase 3 trial. British Journal of Cancer, 120(4), 387-397.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!