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Ysi 2300 stat analyzer

Manufactured by Yellow Springs Instruments
Sourced in United States

The YSI 2300 Stat Analyzer is a laboratory instrument that measures the concentration of specific analytes in a sample. It is designed to provide accurate and reliable results for a variety of applications.

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5 protocols using ysi 2300 stat analyzer

1

Blood lactate analysis during exercise

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All samples were obtained while subjects were seated. The right index finger was cleaned using alcohol prior to each blood draw; the first blood drop was discharged to avoid contamination of the sample. Blood samples of 25 μL were collected in heparinized capillary tubes and transferred to microtubes containing 50 μL of sodium fluoride at 1%. All samples were collected at the following time-points: pre-test, immediately post, 5-min, and 10-min post each protocol. Lactate concentration was analyzed via an electro enzymatic method with a lactate analyzer (YSI 2300 Stat Analyzer; Yellow Springs Instruments, Yellow Springs, OH, United States) previously calibrated, with results expressed in mmol/l. Of note was the fact that values from immediately post, 5 min, and 10 min post were not statistically different, and they were collectively presented only as post-training. Values were also analyzed in duplicate.
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2

Lactate Concentration Quantification

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Blood samples (25 µl) from the fingertips were collected in heparinized capillary tubes and transferred to microtubes containing 50 µL of sodium fluoride at 1%. The lactate concentration was analyzed via an electro-enzymatic method with a lactate analyzer (YSI 2300 Stat Analyzer®; Yellow Springs Instruments, Yellow Springs, OH, USA). Lactate concentrations in blood are expressed in millimoles (mM).
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3

Evaluating Blood Lactate Levels

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To determine the metabolic response of the training session, we evaluated the blood lactate concentrations before and after the training session. Blood samples (25 µL) were collected from the fingertips into heparinized capillary tubes and transferred to microtubes containing 50 µL of 1% sodium fluoride. The lactate concentration was analyzed via an electro-enzymatic method with a lactate analyzer (YSI 2300 Stat Analyzer, Yellow Springs Instruments, Yellow Springs, OH, USA). Blood lactate concentrations are expressed in millimoles (mM).
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4

Blood Sampling for Treadmill Exercise

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A total of 25 µL of blood was collected from the tail on the first and last adaption day on the treadmill, before and after the exhaustive exercise season. Immediately after collection, the sample of blood was transferred to a tube with 50 µL of sodium chloride, and this sample was read in a YSI 2300 Stat Analyzer (Yellow Springs Instruments, Yellow Springs, Greene County, OH, USA) [20 (link)].
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5

Lactate Concentration Analysis in Murine Blood

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Blood from the tail vein of the mice was collected at rest and at the end of the
acute running session. The samples (25 µL) were treated with 1% (w/v) of sodium
fluoride (Sigma Aldrich), and the lactate concentration was analyzed using an
electroenzymatic method with the lactate analyzer YSI 2300 Stat Analyzer (Yellow
Springs Instruments, USA).
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