Floxed stop chr2 tdtomato

All mouse care and experimental procedures were approved by the institutional animal care research advisory committee of the Albert Einstein College of Medicine. All experiments were performed in accordance with relevant guidelines and regulations. Mice used in experiments included Th-Cre (Stock# 008601), floxed tdTomato (Stock# 007909), and floxed-stop ChR2-tdTomato (Stock# 012567) transgenic mice (The Jackson Laboratory). Both male and female mice on a mixed C57BL6/129SVJ background were used for all experiments. Animals were housed in groups in cages under conditions of controlled temperature (22 °C) with a 12:12 hr light-dark cycle and fed a standard chow diet with ad libitum access to water.

Mice used in this study included POMC-Cre (Stock #005965), floxed-stop GFP (Stock #004077), and floxed-stop ChR2-tdTomato (Stock #012567) transgenic mice (The Jackson Laboratory). Both female and male mice of mixed C57BL/6J, FVB, and 129 strain backgrounds were used. Animals were housed in groups in cages under conditions of controlled temperature (22°C) with a 12:12 h light-dark cycle and fed a standard chow diet with ad libitum access to water.