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2 protocols using optigrade

1

Anthocyanin Standards Preparation

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Acetonitrile and methanol (Promochem Optigrade, LGC Standards GmbH, Wesel, Germany) were gradient grades for liquid chromatography. Delphinidin-3-O-glucoside (Dph-glc), Cyanidin-3-O-glucoside (Cyd-glc), Pelargonidin-3-O-glucoside (Plg-glc), Petunidin-3-O-glucoside (Ptd-glc), Malvidin-3,5-di-O-glucoside (Mvd-diglc), Peonidin-3-O-glucoside (Pnd-glc), and Malvidin-3-O-glucoside (Mvd-glc) were purchased from Extrasynthese (Genay, France). Formic acid 98–100% was purchased from Molar Chemicals Ltd., Halásztelek, Hungary. High-purity deionized water was obtained by a LaboStar 7 TWF-UV ultrapure water system (SG Wasseraufbereitung und Regenerierstation GmbH, Barsbüttel, Germany).
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2

Optimized Flavonoid Compound Extraction

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Acetonitrile and methanol (Promochem Optigrade, LGC Standards GmbH, Wesel, Germany) were gradient grade for liquid chromatography. Quercetin-3-O-rutinoside, quercetin-3-O-galactoside, quercetin-3-O-glucoside, quercetin-3-O-glucuronide, kaempferol-3-O-glucoside, and kaempferol-3-O-glucuronide were purchased from Extrasynthese (Genay, France). Ultrapure water system (LaboStarf 7 TWF-UV, Germany) was used to obtain high purity water.
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