Slic3r

Cell-laden composite bioinks (3% MeALG with 1% bone particles) were printed on methacrylated glass slides [96 (link)] at optimized bioprinting parameters, such as 150–200 kPa at 20–30 mm/s for neat inks, and 150–230 kPa at 20–30 mm/s for composite inks. 3D scaffold designs were created by Autodesk^® Fusion 360™ and the 3D models were sliced with Slic3r in Repetier-Host to generate G-code files. A 2 mm × 2 mm grid scaffold composed of 4-layers (with a layer height of 150-µm and 500-µm offset between struts) was used for culture studies. Each bioprinted layer was partially crosslinked for 15 s to allow formation of a self-supporting layer (405 nm, 2 mW/cm²). Bioprinted scaffolds were exposed to light for 1 min to finalize the bioprinting process. Bioprinted cell-laden scaffolds were immediately transferred into non-treated 6-well plates, and 5 mL of growth media was added into each well.

Cylinders of 13 mm in diameter and 1 mm in height were graphically designed and sliced using free versions of Tinkercad^® (Tinkercad.com/">https://www.Tinkercad.com/, Autodesk^® Inc., San Rafael, CA, USA) and Slic3r^® (version 1.3.0, Rome, Italy) software, respectively. FDM Voolt 3D model Gi3 printer (Sao Paulo, Brazil) endowed with a nozzle diameter of 0.4 mm was used to print the filaments chosen in Section 2.2. The printing temperature was 185 °C for the first layer and 180 °C for other layers. The temperature of the building platform was set at 65 °C, and five devices were printed at a time. The layer height was fixed at 0.2 mm, the infill density was 10% using a rectilinear pattern, and the printing speed was 15 mm s⁻¹ for printing moves and 50 mm s⁻¹ for travel speed.