Recombinant mouse ifn γ 485 mi cf

Manufactured by R&D Systems

Recombinant mouse IFN-γ (485 MI/CF) is a laboratory-produced protein that mimics the function of the naturally occurring mouse interferon gamma (IFN-γ) cytokine. It is commonly used in research applications to study immune system responses and cellular signaling pathways.

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Lab products found in correlation

Dimethyloxalylglycine dmog, by Cayman Chemical (2 mentions) Citrulline, by Merck Group (1 mentions) Ascorbate, by Merck Group (1 mentions) Hif 1α nb100 479, by Novus Biologicals (1 mentions) Il 1b af 401 na, by R&D Systems (1 mentions) β actin 13e5, by Cell Signaling Technology (1 mentions) S nitroso n acetylpenicillamine snap, by Merck Group (1 mentions) U 13c glucose, by Cambridge Isotopes (1 mentions) 2 deoxyglucose 2 dg, by Merck Group (1 mentions) D glucose, by Merck Group (1 mentions)

2 protocols using recombinant mouse ifn γ 485 mi cf

Mouse Interferon-gamma Signaling Assay

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Recombinant mouse IFN-γ (485 MI/CF) was obtained from R&D systems. 1400W, and Dimethyloxalylglycine (DMOG) were obtained from Cayman Chemical. Pam3CysK4 (PAM) was obtained from EMC Microcollections. Ascorbate, citrulline, and S-Nitroso-N-acetylpenicillamine (SNAP) were obtained from Sigma-Aldrich. The following primary antibodies were used: HIF-1α (NB100-479, Novus Biologicals), IL-1b (AF-401-NA, R&D systems), and the following Cell Signaling Technology antibodies: HIF-1α (D2U3T), RelA (D14E12), RelB (C1E4), NF-kB1 (D4P4D), IkBa (L35A5), α/β-Tubulin (2148), Histone H3 (D1H2), and β-actin (13E5).

Braverman J, & Stanley S.A. (2017). Nitric oxide modulates macrophage responses to M. tuberculosis infection through activation of HIF-1α and repression of NF-kB. Journal of immunology (Baltimore, Md. : 1950), 199(5), 1805-1816.

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Metabolic Regulation by IFN-γ

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Recombinant mouse IFN-γ (485 MI/CF) was obtained from R&D systems, Minneapolis, MN, and was used at indicated concentrations. 2-deoxyglucose (2-DG) and D-glucose were obtained from Sigma Aldrich, St Louis, MO and were used at indicated concentrations. U-¹³C-glucose was obtained from Cambridge Isotope Laboratories, Andover, MA. Dimethyloxalylglycine (DMOG) was obtained from Cayman Chemical, Ann Arbor, MI and was used at indicated concentrations.

Braverman J., Sogi K.M., Benjamin D., Nomura D.K, & Stanley S.A. (2016). HIF-1α is an essential mediator of IFN-γ dependent immunity to Mycobacterium tuberculosis. Journal of immunology (Baltimore, Md. : 1950), 197(4), 1287-1297.

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