Levamisole

DEFs were obtained from the American Type Culture Collection (VA). Cell cultures were grown in eagle's minimum essential medium (American Type Culture Collection) containing 10% fetal bovine serum (Gibco, NY). Cell cultures were maintained in an incubator at 37°C with 95% humidity and 5% of CO₂. Saline was purchased from Kelun Pharmaceutical Co., Ltd. (Henan, China), dihydromyricetin, ribavirin, chlorogenic acid, naringen, imiquimod, thymopentin, thymosin α1, propolis, β-D-Glucan, astragalus polysacharin, astragalus saponin I, astragalus flavone, lycium barbarum polysaccharide, and levamisole were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Mannan peptide was purchased from National Institutes for Food and Drug Control (Beijing, China); curcumin and LPS were purchased from Sigma-Aldrich (St. Louis, MO); CpG-DNA-1 (5′-ATGGCGTTGACGTTT-3′) and CpG-DNA-2 (5′-GATATGCGACCGATT-3′) were obtained from TSINGKE Biological Technology (Beijing, China); human IgG and chicken IgG were purchased from Solarbio (Beijing, China); and poly(I:C) was purchased from InvivoGen (San Diego, CA). All reagents were dissolved in sterile water, and the stock solution was kept at −20°C and protected from light until use.

The bright green fluorescent dots were measured on day 1 of adult TJ356 strain which was
incubated on NGM/HT115 plates after treated with or without RNAi bacteria from birth.
After 25 minutes of heat stress at 37°C, worms were placed on a 2% agarose pad and
paralyzed with levamisole (Yuanye, China). The expression of GFP was detected by
fluorescence microscopy. The intensity of fluorescence was analyzed using ImageJ.