Surveyor autosampler plus

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Surveyor Autosampler Plus is a robust and reliable laboratory automation solution designed to enhance the efficiency and productivity of analytical workflows. It features a high-capacity sample tray, precise and consistent sample handling, and seamless integration with various analytical instruments. The Surveyor Autosampler Plus provides reliable and repeatable sample introduction, ensuring accurate and reliable data generation for your laboratory operations.

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6 protocols using surveyor autosampler plus

Molecular Networking of LC-MS/MS Data

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LC-MS/MS data for molecular networking were obtained with a system consisting of a Thermo Finnigan Surveyor Autosampler Plus, LC-Pump-Plus and PDA Plus coupled a Thermo Finnigan LCQ Advantage Max mass spectrometer. The flash chromatography fractions were dissolved in MeOH to a concentration of 1 mg/mL, and 20 µL of each fraction was injected onto a Kinetex C18 column (5 µm, 4.6 mm × 100 mm) (Phenomenex, Torrance, CA, USA). The mobile phase consisted of acetonitrile (ACN) and H₂O (both containing 0.1% formic acid) with a flow of 0.7 mL/min, and the components were eluted with the following gradient: 30% ACN for 5 min, increase to 99% ACN over 17 min, hold at 99% ACN for 4 min. The MS was run in positive electrospray, and data from m/z 190 to 2000 was recorded with automated full dependent MS/MS scan enabled. The chromatograms were converted to .mzxml files using msConvert (www.proteowizard.sourceforge.net), and the chromatograms were submitted to GNPS for analysis (www.gnps.ucsd.edu). Cytoscape 3.6.0 (www.cytoscape.org) was used to visualize the molecular networks. A cosine value of 0.7 was used to generate the molecular network.

Kristoffersen V., Rämä T., Isaksson J., Andersen J.H., Gerwick W.H, & Hansen E. (2018). Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group. Marine Drugs, 16(5), 163.

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Chiral Compound Analysis by CD and HPLC

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ECD and UV spectra were measured on a JASCO J-815 CD spectrometer (JASCO, Inc., MD, USA (Flawil, Switzerland) . HPLC was performed with the Thermo Finnigan Surveyor Autosampler Plus using the following column: CHIRALCEL OD-H @ 5 μm (4.6 mmφ Χ 250 mmL) (Massachusetts, USA).

Lakornwong W., Kanokmedhakul K., Masranoi J., Tontapha S., Yahuafai J., Laphookhieo S., Suthiphasilp V, & Kanokmedhakul S. (2022). Cytotoxic and antibacterial xanthones from the roots of Maclura cochinchinensis. Natural product research, 36(23).

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Robenidine Hydrochloride Quantification Protocol

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Robenidine Hydrochloride (Purity ≥ 98%, Quality ratio, Dr. Ehrenstorfer GmbH, Augsburg, Germany), Robenidine-D8 Standard (Purity ≥ 98%, Quality ratio, Dr. Ehrenstorfer GmbH, Augsburg, Germany), and Robenidine Hydrochloride Raw Material (CAS 25875-50-7, Purity 98%, Shouguang Huachih Chemical Co., Shandong, China) were used. Ethyl acetate, hexane, and formic acid (chromatographic purity, J.T. Baker Co., NJ, USA). Anhydrous ethanol (analytically pure, Xilong Chemical Co., Ltd., Fujian, China), distilled water, soluble starch (analytically pure, Xiangzhong Chemical Reagent Supply Station, Shanghai, China), anhydrous magnesium sulfate (analytically pure, Sinopharm Chemical Reagent Co., Ltd., Shanghai, China), and heparin sodium (biological grade, Shanghai Biochemical Reagent Co., Ltd., Shanghai, China). Mettler-TOLE-DOAE-240 precision electronic balance (Mettler-Toledo, Zurich, Switzerland), 20PR-520 high-speed refrigerated centrifuge (Hitachi, Japan), speed mixer (Shanghai Kanghua, Shanghai, China), nitrogen blower (AOSHENG, Hangzhou Aosheng Instrument Co., Ltd., Zhejiang, China), rotary evaporator (Gongyi Yuhua, Henan, China), HPLC–Tandem Mass Spectrometer (Surveyor MS Pump Plus, Surveyor Autosampler Plus, Thermo TSQ Quantum Access MAX and Thermo LCquan 2.6 data acquisition system, Thermo Fisher Scientific, MA, USA).

Zhang L., Du X., Ai X, & Liu Y. (2023). Mass Balance Studies of Robenidine Hydrochloride in the Body of Channel Catfish (Ictalurus punctatus). Animals : an Open Access Journal from MDPI, 13(23), 3745.

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Quantitative Steroid Analysis in Colon

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For quantitative analysis of steroids, longitudinal sections of the colon (200 mg/sample) were collected, and internal standards, such as 17β-Estradiol-2,3,4-¹³C₃ (2 ng/sample), progesterone-2,3,4,20,25¹³C₅-PROG (0.4 ng/sample), and pregnenolone-20,21-¹³C₂-16,16 D₂ (10 ng/sample), were added. The tissue was processed as previously described [39 (link)]. Briefly, the colon tissues were homogenized using the Tissue Lyser (Qiagen, Milan, Italy), in ice cold MeOH/Acetic acid 1% and purified by organic phase extraction as previously described [45 (link)]. The quantitative analysis was performed using a linear ion trap-mass spectrometer (LTQ, Thermo Fisher Scientific, Waltham, MA, USA) equipped with a Surveyor liquid chromatography (LC) Pump Plus and a Surveyor Autosampler Plus (Thermo Fisher Scientific, Waltham, MA, USA), operating in APCI positive mode. Chromatographic separation was achieved on an Hypersil Gold column C18 (100 × 2.1 mm, 3 μm, Thermo Fisher Scientific, Waltham, MA, USA). LC–MS/MS data were evaluated using Excalibur^® release 2.0 SR2 (Thermo Fisher Scientific, Waltham, MA, USA). Quantitative analysis of PREG, PREG sulphate, PROG, DHP, ALLO, ISOALLO, DHEA, T, DHT, 3α-diol, and 17β-E were achieved based on calibration curves which were freshly prepared.

Diviccaro S., FitzGerald J.A., Cioffi L., Falvo E., Crispie F., Cotter P.D., O’Mahony S.M., Giatti S., Caruso D, & Melcangi R.C. (2022). Gut Steroids and Microbiota: Effect of Gonadectomy and Sex. Biomolecules, 12(6), 767.

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HPLC-MS/MS Analytical Protocol for Quantification

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For the analysis a Surveyor ® Autosampler Plus with a quaternary MSpump plus and degasser (ThermoFischer, Breda, The Netherlands) as a chromatographic system were used. A TSQ Quantum-Access ® triplequad mass spectrometer (ThermoFischer, Breda, the Netherlands) with an electrospray ionization interface combined with Excalibur ® software (version 2.2SP1) was used for detection and quantification.
Chromatographic separation was performed on a HyPURITY ® C 18 analytical column (50 × 2.1 mm, 3 μm, Thermo Fischer Scientific) combined with a drop-in guard (HyPURITY ® C 18 , 10 × 2.1 mm, 3 μm).

van Veelen A., van Geel R., de Beer Y., Dingemans A.M., Stolk L., Ter Heine R., de Vries F, & Croes S. (2020). Validation of an analytical method using HPLC-MS/MS to quantify osimertinib in human plasma and supplementary stability results. Biomedical chromatography : BMC, 34(4).

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Quantitative Lipid Profiling by Mass Spectrometry

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Analysis of phospholipids containing a choline headgroup (PC, lyso-PC, SPH) and of PE was performed as described before [21, 27] , using a Thermo TSQ Quantum Discovery MAX triple quadrupole mass spectrometer (Thermo, Dreieich, Germany) equipped with a heated electrospray ionization (H-ESI) interface. In brief, 25µL sample (6µmol/L phospholipid in butanol:methanol:water (75:23:2,v/v)) containing dieicosanoyl-PC and dimyristoyl-PC as internal standards were introduced via loop injection using a Finnigan Surveyor Autosampler Plus and MS Pump Plus (Thermo, Dreieich, Germany), and a mobile phase comprising butanol:methanol:water:25% NH 4 OH (75:23:1.7:0.3;v/v). PC species were quantified from precursor ions of the respective diagnostic fragment ions. These were phosphocholine for endogenous PC, lyso-PC and SPH (mass / charge (m/z)=184), D 9 -phosphocholine (m/z=193) for PC synthesized de novo by direct [methyl-D 9 ]choline incorporation, and D 3 -phosphocholine (m/z=187) and D 6 -phosphocholine (m/ z=190) for PC synthesized by sequential PE N-methylation to yield PC. Here, sequential transfer of one or two deuterated methyl groups (CD 3 -) from D 3 -S-adenosylmethionine originating from [D 9 -methyl]-choline catabolism leads to mass shifts of PC species of M+3 and M+6. PE species were analyzed from the neutral loss fragment phosphoethanolamine (m/z=+141) of the individual masses.

Grothe J., Riethmüller J., Tschürtz S.M., Raith M., Pynn C.J., Stoll D, & Bernhard W. (2015). Plasma phosphatidylcholine alterations in cystic fibrosis patients: impaired metabolism and correlation with lung function and inflammation. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 35(4).

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