Western blotting to analyze protein expression was performed as previously described44 (link), 45 (link). Briefly, cell pellets and tissues were lysed using IP lysis buffer (Fisher Scientific) and the protein concentration was determined by a Bicinchoninic Acid Protein Assay (Thermo Scientific) using a FLUOstar Omega microplate reader (BMG Labtech). 25 μg of protein was used for immunoblot analysis using antibodies against DHODH (1:1,000, 14877-1-AP, Proteintech), GPX4 (1:1,000, MAB5457, R&D systems), vinculin (1:3,000, V4505, Sigma-Aldrich), SLC7A11 (1:1,000, 12691, Cell Signaling), ACSL4 (1:1,000, sc-271800, Santa Cruz), COQ2 (1:1,000, sc-517107, Santa Cruz Biotechnology), FSP1 (1:1,000, sc-377120, Santa Cruz Biotechnology), and Flag (1:10,000, 66008-3-Ig, Proteintech).
Sc 517107
Manufactured by Santa Cruz Biotechnology
Sc-517107 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for use in scientific research and experiments. The core function of this product is to [SPECIFIC CORE FUNCTION]. No further details or interpretation about the intended use of this product are provided.
Automatically generated - may contain errors
Lab products found in correlation
Bicinchoninic acid protein assay, by Thermo Fisher Scientific (2 mentions)
Sc 271800, by Santa Cruz Biotechnology (2 mentions)
Mab5457, by R&D Systems (2 mentions)
Sc 377120, by Santa Cruz Biotechnology (2 mentions)
V4505, by Merck Group (2 mentions)
Fluostar omega microplate reader, by BMG Labtech (2 mentions)
Ip lysis buffer, by Thermo Fisher Scientific (2 mentions)
2 protocols using sc 517107
1
Protein Expression Analysis by Western Blot
2
Western Blotting Analysis of Mitochondrial Proteins
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Western blotting was used to analyze protein expression in cells as described previously (52 (link), 53 (link)). In brief, cell pellets were lysed using IP lysis buffer (Thermo Fisher Scientific), and the protein concentrations were measured using a bicinchoninic acid protein assay (Thermo Fisher Scientific) with a FLUOstar Omega microplate reader (BMG LABTECH). Mitochondrial protein was extracted by using a mitochondrial fractionation kit (K256; BioVision). Thirty micrograms of protein was used for immunoblot analysis with antibodies against GPD1 (1:1,000,13451-1-AP; Proteintech), GPD1L (1:1,000, 17263-1-AP; Proteintech), GPD2 (1:1,000, 17219-1-AP; Proteintech), GPX4 (1:1,000, MAB5457; R&D Systems), DHODH (1:1,000, 14877-1-AP; Proteintech), SLC7A11 (1:1,000, 12691; Cell Signaling Technology), ACSL4 (1:1,000, sc-271800; Santa Cruz Biotechnology), COQ2 (1:1,000, sc-517107; Santa Cruz Biotechnology), FSP1 (1:1,000, sc-377120; Santa Cruz Biotechnology), and vinculin (1:3,000, V4505; Sigma-Aldrich).
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