Total RNA samples extracted from peripheral blood of three patients and three unrelated male controls were used to build cDNA libraries using the TruSeq®Stranded Total RNA LT- kit (with Ribo-Zero TM Gold) (Illumina, USA). Sequencing was performed on the NextSeq 500 platform Mid Output v2 Kit (150 cycles) (Illumina, USA). The FASTQ files were aligned against the ribosomal reference sequence (NCBI, 12/2017) using the BWA software [26] version 0.7.17-r1188, in MEM mode, with the standard parameters, except for the -t 4 parameters. Reads not aligned to ribosomal sequences went to the alignment step against the reference sequence of the human genome (version GRCh37 - hg19) using the STAR software [27], version 2.6.1a_08–27. The annotation database (GTF file) used was the Ensembl file in version 87 in the same build as the human genome reference (GRCh37).
Nextseq 500 platform mid output v2 kit
Manufactured by Illumina
Sourced in United States
The NextSeq 500 platform Mid Output v2 Kit is a laboratory equipment product designed for DNA sequencing. It provides a set of reagents and consumables necessary for the operation of the NextSeq 500 sequencing system, allowing for the analysis of genetic samples.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nextseq 500 platform mid output v2 kit
1
Transcriptome Analysis of Peripheral Blood
2
RNA-seq Analysis of Blood Transcriptome
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Total RNA samples extracted from peripheral blood of three patients and three unrelated male controls were used to build cDNA libraries using the TruSeq®Stranded Total RNA LT-kit (with Ribo-Zero TM Gold) (Illumina, USA). Sequencing was performed on the NextSeq 500 platform Mid Output v2 Kit (150 cycles) (Illumina, USA). The FASTQ files were aligned against the ribosomal reference sequence (NCBI, 12/2017) using the BWA software [26] (link) version 0.7.17-r1188, in MEM mode, with the standard parameters, except for the -t 4 parameters.
Reads not aligned to ribosomal sequences went to the alignment step against the reference sequence of the human genome (version GRCh37 -hg19) using the STAR software [27] (link), version 2.6.1a_08-27. The annotation database (GTF file) used was the Ensembl file in version 87 in the same build as the human genome reference (GRCh37).
Reads not aligned to ribosomal sequences went to the alignment step against the reference sequence of the human genome (version GRCh37 -hg19) using the STAR software [27] (link), version 2.6.1a_08-27. The annotation database (GTF file) used was the Ensembl file in version 87 in the same build as the human genome reference (GRCh37).
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