The presence of PKH67-stained LV9 on various B cell subsets was assessed using the following antibodies: anti-CD21-phycoerythrin (PE)-CF594 (clone 7G6; BD Biosciences), anti-CD23-allophycocyanine (APC) (clone B3B4; BioLegend), anti-CD45.1-PE (clone A20; BD Biosciences), anti-CD45.2-APC-Cy7 (clone 104; BioLegend), anti-CD86-BV510 (clone GL1; BD Biosciences), and anti-MHCII-BV421 (clone M5/114.15.2; BD Biosciences). To conserve the phosphorylation status during the measurement of type I IFN signaling, cells were fixed in 2% PFA prior to permeabilization using cold methanol and staining using the following antibody panel: anti-CD21-PE-CF594 (clone 7G6; BD Biosciences), anti-CD23-PE-Cy7 (clone B3B4; BD Biosciences), anti-MHCII-fluorescein isothiocyanate (FITC) (clone 2G9; BD Biosciences), and anti-pSTAT1-AF647 (clone 4a; BD Biosciences). For each sample, 100,000 cells were acquired using an LSRFortessa cell analyzer (BD), and data were analyzed using FlowJo software (BD).
Anti cd21 phycoerythrin pe cf594 clone 7g6
Manufactured by BD
Anti-CD21-phycoerythrin (PE)-CF594 (clone 7G6) is a fluorescent-labeled antibody that binds to the CD21 receptor on the surface of B cells. It can be used for the identification and enumeration of B cells in flow cytometry applications.
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Lab products found in correlation
Lsrfortessa cell analyzer, by BD (2 mentions)
Anti cd45.2 apc cy7 clone 104, by BioLegend (2 mentions)
Anti mhcii bv421 clone m5 114.15.2, by BD (2 mentions)
Anti cd23 allophycocyanine apc clone b3b4, by BioLegend (2 mentions)
Anti cd45.1 pe clone a20, by BD (2 mentions)
Anti cd86 bv510 clone gl1, by BD (2 mentions)
Anti cd21 pe cf594 clone 7g6, by BD (2 mentions)
Anti cd23 pe cy7 clone b3b4, by BD (2 mentions)
Anti mhcii fluorescein isothiocyanate fitc clone 2g9, by BD (2 mentions)
Anti pstat1 af647 clone 4a, by BD (2 mentions)
2 protocols using anti cd21 phycoerythrin pe cf594 clone 7g6
1
Flow Cytometric Analysis of IFN-Signaling in B Cells
2
Flow Cytometric Analysis of IFN-Signaling in B Cells
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The presence of PKH67-stained LV9 on various B cell subsets was assessed using the following antibodies: anti-CD21-phycoerythrin (PE)-CF594 (clone 7G6; BD Biosciences), anti-CD23-allophycocyanine (APC) (clone B3B4; BioLegend), anti-CD45.1-PE (clone A20; BD Biosciences), anti-CD45.2-APC-Cy7 (clone 104; BioLegend), anti-CD86-BV510 (clone GL1; BD Biosciences), and anti-MHCII-BV421 (clone M5/114.15.2; BD Biosciences). To conserve the phosphorylation status during the measurement of type I IFN signaling, cells were fixed in 2% PFA prior to permeabilization using cold methanol and staining using the following antibody panel: anti-CD21-PE-CF594 (clone 7G6; BD Biosciences), anti-CD23-PE-Cy7 (clone B3B4; BD Biosciences), anti-MHCII-fluorescein isothiocyanate (FITC) (clone 2G9; BD Biosciences), and anti-pSTAT1-AF647 (clone 4a; BD Biosciences). For each sample, 100,000 cells were acquired using an LSRFortessa cell analyzer (BD), and data were analyzed using FlowJo software (BD).
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