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Amicon ultra 10 000 mwco centrifugal filter units

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The Amicon Ultra® 10,000 MWCO centrifugal filter units are a type of lab equipment used for ultrafiltration. They are designed to separate and concentrate macromolecules, such as proteins, from solutions based on their molecular weight. The units feature a 10,000 molecular weight cut-off (MWCO), which means they can retain molecules larger than 10,000 Daltons while allowing smaller molecules to pass through.

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Lab products found in correlation

Ni nta, by Qiagen (2 mentions) Tev protease, by Thermo Fisher Scientific (2 mentions) Ni nta, by Thermo Fisher Scientific (2 mentions) Hiload 16 60 superdex 200, by GE Healthcare (2 mentions)

Spelling variants of this product

Amicon centrifugal filters (166 citations) Centrifugal filter units (146 citations) Amicon Ultra filters (132 citations) Amicon filter units (21 citations) Amicon centrifugal units (20 citations) Amicon Ultra centrifugal units (17 citations) MWCO centrifugal filter (11 citations) MWCO-10,000 (8 citations) Amicon Ultra (10,000 MWCO) (5 citations) Filter units (3 citations)

2 protocols using amicon ultra 10 000 mwco centrifugal filter units

1

Purification and Characterization of CtIP Protein Domains

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Sequences corresponding to human CtIP residues 1–145, 18–145, 18–52, 52–145 and 769–897, including point mutants L27E, F20E, double mutant C89A,C92A and Δ20-27, were cloned into pMAT11 vectors38 (link) for expression as N-terminal His6-MBP fusion proteins. Constructs were expressed in BL21(DE3)Rosetta2 cells (Novagen®), in 2xYT media, induced with 0.5 mM IPTG for 16 hours at 20°C. Fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen) and amylose (NEB) affinity chromatography. Affinity tags were removed by cleavage with TEV protease (Invitrogen) and capture on to Ni-NTA resin. Further purification was achieved through ion-exchange chromatography (Resource S for CtIP-nNTD, Resource Q for all other samples: GE Healthcare) and size-exclusion chromatography (HiLoad™ 16/60 Superdex 200: GE Healthcare) in 20 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT. Protein samples were concentrated to 10 mg/ml using Amicon Ultra® 10,000 MWCO centrifugal filter units (Millipore), and were stored at −80°C following flash-freezing in liquid nitrogen.
Davies O.R., Forment J.V., Sun M., Belotserkovskaya R., Coates J., Galanty Y., Demir M., Morton C.R., Rzechorzek N.J., Jackson S.P, & Pellegrini L. (2015). CtIP tetramer assembly is required for DNA-end resection and repair. Nature structural & molecular biology, 22(2), 150-157.
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2

Purification and Characterization of CtIP Protein Domains

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sequences corresponding to human CtIP residues 1–145, 18–145, 18–52, 52–145 and 769–897, including point mutants L27E, F20E, double mutant C89A,C92A and Δ20-27, were cloned into pMAT11 vectors38 (link) for expression as N-terminal His6-MBP fusion proteins. Constructs were expressed in BL21(DE3)Rosetta2 cells (Novagen®), in 2xYT media, induced with 0.5 mM IPTG for 16 hours at 20°C. Fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen) and amylose (NEB) affinity chromatography. Affinity tags were removed by cleavage with TEV protease (Invitrogen) and capture on to Ni-NTA resin. Further purification was achieved through ion-exchange chromatography (Resource S for CtIP-nNTD, Resource Q for all other samples: GE Healthcare) and size-exclusion chromatography (HiLoad™ 16/60 Superdex 200: GE Healthcare) in 20 mM Tris pH 8.0, 150 mM NaCl, 2 mM DTT. Protein samples were concentrated to 10 mg/ml using Amicon Ultra® 10,000 MWCO centrifugal filter units (Millipore), and were stored at −80°C following flash-freezing in liquid nitrogen.
Davies O.R., Forment J.V., Sun M., Belotserkovskaya R., Coates J., Galanty Y., Demir M., Morton C.R., Rzechorzek N.J., Jackson S.P, & Pellegrini L. (2015). CtIP tetramer assembly is required for DNA-end resection and repair. Nature structural & molecular biology, 22(2), 150-157.
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