Overnight cultures of A. baumannii were used to inoculate 5 ml of LB in 50-ml conical tubes at a 1:100 dilution. Sterile polystyrene coverslips were placed in the inoculated 50-mL conical tubes and the tubes were incubated for 48 h at 37°C without shaking as previously described [22 (link)]. Coverslips were washed, dehydrated in ethanol, processed with a critical point drier, and sputter coated as described previously [18 (link)]. Biofilms formed above, at and below the liquid-air interface were viewed using a Zeiss Supra Gemini Series 35V scanning electron microscope as described previously [18 (link)].
Supra gemini series 35v
Manufactured by Zeiss
The Supra Gemini Series 35V is a high-performance scanning electron microscope (SEM) manufactured by Zeiss. It is designed to provide high-resolution imaging and advanced analytical capabilities for a wide range of applications in materials science, nanotechnology, and life sciences research. The core function of the Supra Gemini Series 35V is to enable users to observe and analyze the microstructure and composition of samples at the nanoscale level with exceptional resolution and image quality.
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Lab products found in correlation
3 protocols using supra gemini series 35v
1
Scanning Electron Microscopy of Acinetobacter baumannii Biofilms
2
Biofilm Formation by A. baumannii
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Overnight cultures of A. baumannii were used to inoculate 5 ml of LB in 50-ml conical tubes at a 1:100 dilution. Sterile polystyrene coverslips were placed in the inoculated 50-mL conical tubes and the tubes were incubated for 48 h at 37°C without shaking as previously described.24 (link) Coverslips were washed, dehydrated in ethanol, processed with a critical point drier, and sputter coated as described previously.21 (link) Biofilms formed above, at and below of the liquid-air interface were viewed using a Zeiss Supra Gemini Series 35V scanning electron microscope as described previously.21 (link)
3
Scanning Electron Microscopy of Acinetobacter baumannii Biofilms
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Overnight cultures (two biological replicates of each strain) of A. baumannii were used to inoculate 5 mL of modified LB-LN in 50 ml conical tubes at a 1:100 dilution. The test medium was supplemented with 0.5% bile salts, and the control medium was not supplemented. Sterile polystyrene coverslips were placed in the inoculated 50 mL conical tubes, which were incubated for 48 h at 37°C without shaking, as previously described (Gaddy et al., 2009 (link)). Coverslips were washed, dehydrated in ethanol, processed with a critical point drier, and sputter-coated, as described above (Tomaras et al., 2003 (link)). Biofilms formed above, at and below the liquid-air interface were viewed in a Zeiss Supra Gemini Series 35 V scanning electron microscope, as previously described (Rey et al., 1989 (link)).
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