To create MED19-AKO mice, we obtained mice with conditional-potential allele of MED19 (MED19tm1a(EUCOMM)Wtsi from the EUCOMM repository and crossed them with actin-Flp transgenic mice to remove the targeting cassette and then subsequently crossed the mice with adiponectin-Cre mice obtained from the Jackson Laboratory (028020) to generate adipose-specific MED19 knockout (MED19-AKO) mice. To generate adipose-specific, tamoxifen-inducible MED19 KO (MED19-iAKO) mice, MED19 floxed animals were crossed with Adiponectin-CreER mice obtained from the Jackson Laboratory (025124). To induce knockout, 12-week-od control (MED19Lox/Lox without Cre) and MED19-iAKO mice were treated daily with tamoxifen (100 μg/g body weight) for five consecutive days. All experiments used male mice unless otherwise stated. All mice were kept on a 12h light/dark cycle and provided ad libitum access to food (Purina 5053) and water. All animal experiments were performed in accordance with procedures approved by the Institutional Animal Care and Use Committee at Washington University School of Medicine.
Adiponectin cre mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
Adiponectin-Cre mice are a genetically modified mouse line that expresses the Cre recombinase enzyme under the control of the Adiponectin promoter. This allows for the specific inactivation or modification of target genes in adipose tissue.
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Lab products found in correlation
Lox stop lox rosa diphtheria toxin mice, by Jackson ImmunoResearch (2 mentions)
C57bl 6j mice, by Jackson ImmunoResearch (2 mentions)
Adiponectin creer mice, by Jackson ImmunoResearch (1 mentions)
Ap2 cre mice, by Jackson ImmunoResearch (1 mentions)
D12492, by Research Diets (1 mentions)
Foxp3egfp mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
F3282, by Bio-Serv (1 mentions)
Mi laboratory rodent diet 5001, by Inotiv (1 mentions)
Echomri 3 in 1, by Echo Medical Systems (1 mentions)
D12451, by Research Diets (1 mentions)
Rodent diet 20, by PicoLab (1 mentions)
Rodent laboratory chow 5001, by Purina (1 mentions)
Dexamethasone sodium phosphate, by Santa Cruz Biotechnology (1 mentions)
Gr floxed mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6 mice, by Charles River Laboratories (1 mentions)
Ucp1 cre mice, by Jackson ImmunoResearch (1 mentions)
Td 88137, by Inotiv (1 mentions)
Hpp750life, by Memmert (1 mentions)
27 protocols using adiponectin cre mice
1
Generating Adipose-Specific MED19 Knockout Mice
2
FGF21 Knockout Mouse Generation
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Male FGF21KO mice generated by Department of Genetic Biochemistry, Kyoto University40 (link) and WT littermates with the same genetic background were used for this study. Klb AdipoKO mice were generated by crossing the βklotho gene floxed mice (Shanghai Nanfang Centre for Model Organisms) with adiponectin-Cre mice (The Jackson Laboratory, stock No. 010803). FGF21 AdipoKO mice were generated by crossing the FGF21 gene floxed mice (Shanghai Nanfang Centre for Model Organisms) with aP2-Cre mice (The Jackson Laboratory, stock No. 005069)60 (link). Both Klb AdipoKO and FGF21 AdipoKO mice were back-crossed with C57BL/6 J background for at least eight generations to ensure the genetic homogeneity. Klb AdipoKO and FGF21 AdipoKO mice were confirmed by genotyping and western blot analysis (Supplementary Fig. 10 ). No randomization of mice was used. These mice were fed with either STC or HFD (Research Diet, containing 45% fat, 20% protein, and 35% carbohydrate [kcal%]). All animals were kept under 12 h light-dark cycles at 22–24 °C, 60–70% humidity with free access to water. The sample sizes of animal experiments were estimated according to previous studies and the known variability of the assays. All animal experimental protocols were approved by the Animal Ethics Committee of the University of Hong Kong.
3
Adipose-Specific Piezo1 Knockout Mice
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Adiponectin-Cre mice were purchased from the Jackson Laboratory (Stock No: 010803). The Piezo1-flox and Piezo1-tdTomato were generously provided by Dr. Ardem Patapoutian at The Scripps Research Institute. To generate adipose-specific Piezo1 knockout mice, Piezo1-flox/flox mice were crossed to Adiponectin-Cre mice. Mice were housed four or five per cage and maintained under a 12 h light/12 h dark cycle at constant temperature (23°C) with ad libitum access to normal laboratory chow (#2920X; Harlan Teklad) and water. For diet-induced obesity, mice were fed a high-fat diet containing 54.8% fat calories, 24.0% carbohydrate calories, and 21.2% protein calories (4.8 kcal/g) (TD.93075; Envigo Inc.) from 4 weeks of age for 30–35 weeks. Body weight was measured biweekly. All mouse studies were conducted in accordance with federal guidelines and were approved by the Institutional Animal Care and Use Committee of the University of California, Irvine.
4
Adipocyte-specific ATGL Knockout Mice
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ATGLflox/flox mice were crossbred with Adiponectin-Cre mice (Eguchi et al., 2011 (link); The Jackson Laboratory; JAX stock number 010803; genetic background: C57BL/6J) to obtain adipocyte-specific ATGL knockout (AAKO) mice. Body composition of AAKO mice was similar as previously published (Schoiswohl et al., 2015 (link)). Body weight was unchanged compared to ATGLflox/flox control mice, but fat mass was ∼1.7-fold higher.
5
Genetically Modified Mouse Models
6
Flox HO-1 and Adipose-Specific Knockout Mice
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The experimental procedures and protocols of this study conform to the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of the University of Mississippi Medical Center (protocol 1283, initial approval 2/2014, reapproved 1/2017).
Studies were performed on male and female Flox HO-1 and adipose-specific HO-1 knockout mice. Flox HO-1 mice are designed to delete exons 3–5 and activate red fluorescent protein (dsRed) upon cre-mediated deletion and maintained on a C57BL/6J genetic background as originally described [22 (link)]. Adiponectin-Cre mice were mice purchased from Jackson Labs (Bar Harbor, ME, USA) and were derived from the originally described colony and bred onto a C57BL/6J background [21 (link)]. Mice which contain both the Adiponectin-Cre and the flox HO-1 alleles are considered knockouts while mice which lack the Adiponectin-Cre and only contain the flox HO-1 allele are considered Flox mice. Mice were housed under standard conditions until 6 weeks of age after which time some mice were switched to a 60% high-fat diet (diet # D12492, Research Diets, Inc., New Brunswick, NJ, USA) other mice were allowed full access to standard laboratory chow. The groups of mice consumed each diet for 30 weeks.
Studies were performed on male and female Flox HO-1 and adipose-specific HO-1 knockout mice. Flox HO-1 mice are designed to delete exons 3–5 and activate red fluorescent protein (dsRed) upon cre-mediated deletion and maintained on a C57BL/6J genetic background as originally described [22 (link)]. Adiponectin-Cre mice were mice purchased from Jackson Labs (Bar Harbor, ME, USA) and were derived from the originally described colony and bred onto a C57BL/6J background [21 (link)]. Mice which contain both the Adiponectin-Cre and the flox HO-1 alleles are considered knockouts while mice which lack the Adiponectin-Cre and only contain the flox HO-1 allele are considered Flox mice. Mice were housed under standard conditions until 6 weeks of age after which time some mice were switched to a 60% high-fat diet (diet # D12492, Research Diets, Inc., New Brunswick, NJ, USA) other mice were allowed full access to standard laboratory chow. The groups of mice consumed each diet for 30 weeks.
7
Generation of adipose-specific Cnot1-AKO mice
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The creation of Cnot1 conditional knockout (Cnot1loxP/loxP) mice has been previously described [29 (link)]. We backcrossed Cnot1loxP/loxP mice with C57BL/6J mice for at least eight generations. Mice were maintained on a 12 h light/dark cycle in a temperature-controlled (22 °C) barrier facility with free access to water and a normal diet (NCD, CA-1, CLEA Japan, Inc., Meguro, Tokyo, Japan). To generate adipose tissue-specific Cnot1-AKO mice, we crossed Cnot1loxP/loxP mice with Adiponectin-Cre mice (The Jackson Laboratory, Stock No: 010803, Bar Harbor, ME, USA). We used Cnot1loxP/loxP mice as controls unless otherwise indicated. Mouse experiments were approved by the Committee of Animal Experiments at Okinawa Institute of Science and Technology Graduate University (2015-122, 7/12/2015; 2012-054, 1/12/2012).
8
Adiponectin Cre-mediated Phb1 Knockout
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Adiponectin Cre mice (stock no: 028020) were purchased from the Jackson Laboratory (Bar Harbor, ME). Phb1‐floxed mice were generated as described.[9 ] Homozygous Phbflox/flox mice were crossed with Adipo Cre mice through several steps to generate AdipoCre+Phb1flox/flox (Phb1adipo−/−) and AdipoCre−Phb1flox/flox littermate controls (defined as WT mice). Phb1adipo−/− and WT mice (male, 7–8 weeks of age) were fed an HFD (60 kcal% fat; D12492; Research Diets, New Brunswick, NJ) for 3 months and sacrificed for analyses. All animal experiments were approved by the National Institute on Alcohol Abuse and Alcoholism Animal Care and Use Committee.
9
Adiponectin-Cre Driven Oct3 Knockout Mice
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Adiponectin-Cre mice were obtained from the Jackson Laboratory (stock NO.010803). Oct3fl/fl mice were generated in the Ying Xu lab (Soochow University), backcrossed with C57BL/6J mice for at least 8 generations, and subsequently intercrossed with Adiponectin-Cre mice. Mice were housed under a 12-hour light/dark cycle (light period 7:00–19:00) at constant temperature (22°C RT). Food and water were available ad libitum. Male mice were 8 to 10 weeks of age when used for experiments.
10
Adipose-Specific ANG2 Knockout Mice
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ANG2 flox/flox mice was provided by Dr. Martin Witzenrath from Charité – Universitätsmedizin Berlin. Adiponectin-Cre mice were purchased from Jackson Laboratory (Jackson Labs, Bar Harbor, ME). To evaluate whether ANG2 from the adipose tissue affects whole-body metabolism, we first created an ANG2 adipose tissue specific knockout mice model (ANG2 aKO) by cross breeding the ANG2 flox/flox to Adiponectin-Cre mice. All mice are housed under controlled temperature (22 °C) 12-hour light (06:00–17:59) to 12-hour dark (18:00–05:59) cycles at Animal Care Facility at Virginia Commonwealth University. For all experiments, male and female mice aged 8-weeks were used for long term HFD (45 kcal% fat, D12451i, Research Diets) feeding.
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