Cell proliferation was determined by the Alarmar Blue assay following the protocol (Sigma, USA). Briefly, cells were seeded in 96-well plates and starved in medium with 1% serum overnight. After 24 h treatment, resazurin was added into culture media at a final concentration of 10%, and then cells were cultured for additional 2 h. Thereafter, cells were washed with phosphate-buffer saline (PBS) three times and then fluorescence intensity was measured using a microplate reader. The excitation and emission wavelength was 530 and 590 nm, respectively.
Alarmar blue assay
Manufactured by Merck Group
Sourced in United States
Alarmar Blue assay is a laboratory product developed by Merck Group. It is a colorimetric assay used to measure the metabolic activity of cells in culture. The assay relies on the reduction of a redox indicator dye, which changes color in response to chemical reduction of growth medium, providing a quantitative measure of cell viability and proliferation.
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2 protocols using alarmar blue assay
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Alamar Blue Cell Proliferation Assay
2
Alamar Blue Assay for Cell Growth
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Cell growth was determined by the Alarmar Blue assay following the manufacture's protocol (Sigma, USA). Briefly, cells were seeded in 96-well plates and starved in medium with 1% serum overnight. After 24 h treatment, resazurin was added into culture media at a final concentration of 10%, and then cells were cultured for additional 2 h. Thereafter, cells were washed with phosphate-buffer saline (PBS) three times and then fluorescence intensity was measured using a microplate reader. The excitation and emission wavelength were 530 and 590 nm, respectively.
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