Ampicillin sodium salt

Manufactured by Research Products International

Sourced in United States

Ampicillin sodium salt is a white to off-white crystalline powder that is used as a bactericidal antibiotic. It is soluble in water and is commonly used in microbiological research and laboratory settings.

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Ampicillin (5 citations)

2 protocols using ampicillin sodium salt

Antibiotic Susceptibility in Planktonic and Biofilm Bacteria

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Antimicrobials were diluted as per manufacturer specifications and stored at -20 °C until needed, and included trimethoprim (Cayman Chemical 16473), ceftriaxone sodium salt (Cayman Chemical 18866), nitrofurantoin (TCI Chemicals N0883), ampicillin sodium salt (Research Products International A40040), and daptomycin (Cayman Chemical 15651). For planktonic bacteria, the minimum inhibitory concentration was determined as to be the lowest concentration that impaired visible growth during an 18 h incubation in TSB-G at 37 °C with aeration. Growth was also assessed by OD₆₀₀ measurements taken every 15 min for 18 h as previously described using a microplate spectrophotometer (Biotek Synergy H1) [62 (link)]. Susceptibility of biofilms to antimicrobials was determined by establishing biofilms as above. After 20 h, supernatants were carefully removed and replaced with fresh TSB-G containing antimicrobials. Biofilms were then incubated for an additional 24 h, and viable CFUs/mL were determined as above.

Gaston J.R., Andersen M.J., Johnson A.O., Bair K.L., Sullivan C.M., Guterman L.B., White A.N., Brauer A.L., Learman B.S., Flores-Mireles A.L, & Armbruster C.E. (2020). Enterococcus faecalis Polymicrobial Interactions Facilitate Biofilm Formation, Antibiotic Recalcitrance, and Persistent Colonization of the Catheterized Urinary Tract. Pathogens, 9(10), 835.

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Comprehensive Protocol for Microbial Analysis

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Magnesium sulfate, triethylammonium acetate buffer, vancomycin, neomycin, amphotericin B, and methylamine solution (40 wt. % in water) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Organic solvents including m ethanol, chloroform, ethanol, 1-butanol and acetonitrile were all HPLC grade purchased from Thermo Fisher Scientific (Waltham, MA, USA). Calcium chloride dihydrate, ammonium chloride, sodium phosphate dibasic anhydrous, potassium phosphate monobasic, acetic acid (glacial), metronidazole, casamino acids (vitamin-free) and sodium chloride were also purchased from Thermo Fisher Scientific. Ampicillin sodium salt and isopropyl-β-D-thiogalactoside (IPTG) were obtained from Research Products International (RPI; Mt. Prospect, IL, USA). Sep-Pak silica cartridges were purchased from the Waters Corporation (Milford, MA, USA). Kinetex 2.6μ C18 100Å column (50×2.1 mm) and Kinetex C8 100Å column (50×2.1 mm) were purchased from Phenomenex (Torrance, CA, USA).

Dosoky N., Chen Z., Guo Y., McMillan C., Flynn C.R, & Davies S.S. (2019). Two-week administration of engineered Escherichia coli establishes persistent resistance to diet induced obesity even without antibiotic pretreatment.. Applied microbiology and biotechnology, 103(16), 6711-6723.

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