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Laminin

Manufactured by Novus Biologicals
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Laminin is a large glycoprotein found in the extracellular matrix and basement membranes of various tissues. It plays a crucial role in cell attachment, differentiation, migration, and signaling processes.

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Lab products found in correlation

Calponin, by Abcam (2 mentions) α tubulin, by Merck Group (1 mentions) Ne per nuclear extraction kit, by Thermo Fisher Scientific (1 mentions) Ab31962, by Abcam (1 mentions) β actin, by Cell Signaling Technology (1 mentions) β catenin, by BD (1 mentions) Lamin a c, by Santa Cruz Biotechnology (1 mentions) Collagen 1, by Cedarlane (1 mentions) Mac 1, by BD (1 mentions) α sma, by Agilent Technologies (1 mentions) Cd105, by BD (1 mentions) Collagen 4, by Abcam (1 mentions) Runx2, by Santa Cruz Biotechnology (1 mentions) Pparγ, by Santa Cruz Biotechnology (1 mentions) Fibronectin, by Abcam (1 mentions) Collagen 1, by Novus Biologicals (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) α sma, by Merck Group (1 mentions) Phosphorylated stat3 tyr705, by Cell Signaling Technology (1 mentions) Smad2 3, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-laminin (8 citations) Laminin-Alexa Fluor 647 (2 citations) Anti-laminin gamma 1 (A5, (2 citations) Anti-LAMININ DyLight 650 antibody (2 citations) Anti-laminin DyLight 650 (1 citations)

4 protocols using laminin

1

Western Blot Analysis of Wnt11, HIF, and ECM Proteins

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Tissue or cell extracts were immunoblotted with antibodies specific for WNT11 (ab31962, Abcam), Wnt11 (#AF2647; R&D Systems), HIF-1α (NB100–105, NB100–134), HIF-2α (NB100–122), Laminin (Novus Biologicals), ERK, β-actin (Cell Signaling), β-catenin (BD BioScience), Lamin A/C (Santa Cruz Biotechnology), α-tubulin (Sigma-Aldrich), MMP-2 (IM33), MMP-9 (IM37) (EMD Millipore), and HA (Covance, Princeton, NJ). For detection of HIF-1α and HIF-2α, nuclear proteins were isolated using the NE-PER nuclear extraction kit according to manufacture’s protocol (Thermo Scientific). Quantification of WNT11, Tubulin or ERK protein expression were done using ImageJ software (NIH).
Mori H., Yao Y., Learman B.S., Kurozumi K., Ishida J., Ramakrishnan S.K., Overmyer K.A., Xue X., Cawthorn W.P., Reid M.A., Taylor M., Ning X., Shah Y.M, & MacDougald O.A. (2016). Induction of WNT11 by hypoxia and hypoxia-inducible factor-1α regulates cell proliferation, migration and invasion. Scientific Reports, 6, 21520.
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2

Multilineage Marker Expression Profiling

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Calponin, pancytokeratins AE1/AE3, collagen IV (Abcam), collagen 1, collagen 2 (Cedarlane), PPARγ, Sox9, Runx2 (Santa Cruz Biotechnology), osteocalcin (AbD Serotec), FGFR3, laminin (Novus Biologicals), CD31, CD44, CD45, CD73, CD105, Mac1 (BD Biosciences), α-SMA, Ki67 (Dako).
Coutu D.L., Mahfouz W., Loutochin O., Galipeau J, & Corcos J. (2014). Tissue Engineering of Rat Bladder Using Marrow-Derived Mesenchymal Stem Cells and Bladder Acellular Matrix. PLoS ONE, 9(12), e111966.
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3

Reagents for Cell Culture Experiments

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Antibodies and reagents were purchased from the following sources: fibronectin (catalog # Ab2413, Abcam, Cambridge, MA, USA), KDEL (catalog # NBP1–97469, Novus Biologicals, Littleton, CO, USA), collagen I (catalog # NB600–408, Novus Biologicals, Littleton, CO, USA), laminin (catalog # NB300–144, Novus Biologicals, Littleton, CO, USA), CHOP (catalog # 13172, Novus Biologicals, Littleton, CO, USA), and GAPDH (catalog # 3683, Cell signaling technology, Danvers, MA, USA), dexamethasone-21-acetate (Spectrum Chemicals, New Brunswick, NJ, USA), minocycline hydrochloride (Enzo Life Sciences, Inc., New York, NY, USA), and PBA (Scandinavian formulas, PA, USA).
Maddineni P., Kasetti R.B., Kodati B., Yacoub S, & Zode G.S. (2021). Sodium 4-Phenylbutyrate Reduces Ocular Hypertension by Degrading Extracellular Matrix Deposition via Activation of MMP9. International Journal of Molecular Sciences, 22(18), 10095.
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4

Immunoblotting of Tissue Proteins

Cited 2 times
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Colonic, ileal, and gastric tissues were collected in fresh carbogenated Krebs buffer. The muscularis externae was separated from the mucosa/submucosa layer by microdissection. Tissues were snap frozen and stored in −80°C until use. Samples were homogenized on ice in lysis buffer supplemented with protease inhibitor cocktails (Sigma-Aldrich). After spinning at 12,000 g at 4°C for 15 minutes, the supernatant proteins were collected and resolved by a standard immunoblotting method [30 (link)–32 (link)]. Antibodies used included phosphorylated STAT3 Tyr705 (Cat#9145), STAT3 (Cat#4904), AKT (Cat#4691), phosphorylated AKT (Cat#4060), MLC2 (#8505), phosphorylated MLC2 (#3671), SMAD2/3 (#8685), and phosphorylated SMAD (#9510) from Cell Signaling (Danvers, MA). SM22 (Cat#ab10135) and calponin (Cat#ab46794) antibodies were purchased from Abcam (Cambridge, UK). Fibronectin (Cat#SC-6952), phosphorylated PKCα (Cat#SC-12356-R), and PKCα (Cat#SC-208) were purchased from Santa Cruz Biotechnology (Dallas, TX). Laminin (Cat#NB300–144SS) antibodies were purchased from Novus Biologicals (Centennial, CO). α-SMA (Cat#A5228) antibodies were purchased from Sigma-Aldrich (St. Louis, MO). Collagen I antibodies (Cat#600-401-103) were purchased from Rockland (Pottstown, PA). All blots were repeated at least 3 times.
Cummins C.B., Gu Y., Wang X., Lin Y.M., Shi X.Z, & Radhakrishnan R.S. (2019). Burn-Induced Impairment of Ileal Muscle Contractility is Associated with Increased Extracellular Matrix Components. Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract, 24(1), 188-197.
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