10-HCPT, and EDCI (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide) were obtained from Sigma Chemical Co. (St Louis, MO, USA). 5-Carbonyl caproic acid and dichloromethane were obtained from Shanghai Topbiochem Ltd. (Shanghai, People's Republic of China). Anhydrous N,N-dimethylformamide (DMF), thiosemicarbazide, and sulfoxide chloride were obtained from Aladdin Industrial Corporation (Shanghai, People's Republic of China). MeO-PEG2000-COOH (molecular weight 2,000 Da) was obtained from Xi'an Ruixi Biological Technology Co. Ltd. (Xi'an, People's Republic of China). Sephadex LH-20 was obtained from Shanghai Yuanye Bio-Technology Co. Ltd. (Shanghai, People's Republic of China). All other chemicals were of analytical grade and were used without further purification.
Sephadex lh 20
Manufactured by Yuanye Bio-Technology
Sourced in China
Sephadex LH-20 is a size-exclusion chromatography gel matrix used for the separation and purification of a variety of organic compounds, including pharmaceuticals, natural products, and other small molecules. It is a hydrophilic, cross-linked dextran-based gel with a porous structure that allows for the separation of molecules based on their size and molecular weight.
Automatically generated - may contain errors
Lab products found in correlation
Meo peg2000 cooh, by Xi’an Ruixi (6 mentions)
10 hcpt, by Merck Group (2 mentions)
Autopol 1 automatic polarimeter, by Rudolph Research Analytical (1 mentions)
Lichroprep rp 18 gel, by Merck Group (1 mentions)
Microsoft time of flight qii mass spectrometer, by Bruker (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Autopol 6, by Rudolph Research Analytical (1 mentions)
Avance 3 500 mhz equipment, by Bruker (1 mentions)
Nicolet is10 spectrometer, by Thermo Fisher Scientific (1 mentions)
Chirascan cd spectrophotometer, by Applied Photophysics (1 mentions)
4 protocols using sephadex lh 20
1
Synthesis of PEGylated 10-HCPT Conjugate
2
Comprehensive Analytical Characterization
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HR-ESI-MS were obtained using a Bruker microsoft time-of-flight QII mass spectrometer (Bruker Daltonics, Fremont, CA, USA). The NMR spectra were recorded using a Bruker AV 600/400 MHz spectrometer (Bruker, Fällanden, Switzerland). Optical rotation was measured using a Rudolph Autopol I automatic polarimeter (Rudolph Research Analytical, Hackettstown, NJ, USA). Column chromatography was performed using silica gel (200–300 mesh) and Sephadex LH-20 (Shanghai Yuanye Biological Technology Co., Ltd., Shanghai, China), and Lichroprep RP-18 gel (40–60 µm) was purchased from Merck KGaA (Darmstadt, Germany). Thin-layer chromatography (TLC) was performed with precoated silica gel GF 254 glass plates (100 mm × 200 mm, Branch of Qingdao Haiyang Chemical Co., Ltd.). All other chemicals and solvents were of analytical grade and used without further purification.
3
Synthesis and Characterization of PEG-Conjugated HCPT Prodrug
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10-HCPT, EDCI (1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide), and MTT (methylthiazolyldiphenyl-tetrazolium bromide) were obtained from Sigma Chemical Co. (St Louis, MO, USA). 5-carbonyl caproic acid and dichloromethane were obtained from Shanghai Topbiochem Ltd. (Shanghai, People’s Republic of China). Anhydrous N,N-dimethylformamide (DMF), thiosemicarbazide, and sulfoxide chloride were obtained from Aladdin Industrial Corporation (Shanghai, People’s Republic of China). MeO-PEG2000–COOH (molecular weight ~2,000 Da) was obtained from Xi’an Ruixi Biological Technology Co. Ltd. (Xi’an, People’s Republic of China). Sephadex LH-20 was obtained from Shanghai Yuanye Bio-Technology Co. Ltd. (Shanghai, People’s Republic of China). All other chemicals were of analytical grade and were used without further purification.
4
Spectroscopic Analysis of Natural Compounds
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The optical rotations and CD spectra were measured on Autopol VI in MeOH (Rudolph Research Analytical, Hackettstown, NJ, USA) and Chirascan CD spectrophotometer in MeOH (Applied Photophysics, Leatherhead, UK), respectively. The IR spectra via KBr pellets and UV spectra were recorded on Nicolet iS10 spectrometer (Thermo Fisher Scientific, Madison, WI, USA) and Shimadzu UV2401PC (Shimadzu, Kyoto, Japan), respectively. NMR spectra (500 MHz for 1H and 125 MHz for 13C) were recorded using Avance III 500 MHz equipment (Bruker, Bremerhaven, Germany). HRFABMS data and HRESIMS data were determined using Fast-atom-bombardment mass spectrometry DFS (Thermo Fisher Scientific, Madison, WI, USA) and Shimadzu LC/MS-IT-TOF mass instrument (Shimadzu, Kyoto, Japan), respectively. Column chromatography was conducted on silica gel (200–300 mesh, Qingdao Puke Abruption Materials Co., Ltd. Qingdao, China) and Sephadex LH-20 (Shanghai Yuanye Bio-Technology Co., Ltd. Shanghai, China). ODS column chromatography was performed using C18 silica gel (Fuji Silysia Chemical Ltd. Kasugai, Japan). TLC was carried out on precoated glass silica gel GF254 plates and compounds were visualized under UV light or via heating silica gel plates sprayed with 5% H2SO4/EtOH.
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