Apc anti mouse cd11b

Manufactured by BD

APC anti-mouse CD11b is a fluorescently labeled antibody that binds to the CD11b cell surface marker expressed on myeloid cells, including monocytes, macrophages, and granulocytes, in mouse samples. It is used for the identification and analysis of these cell populations in flow cytometry applications.

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Lab products found in correlation

Anti mouse cd3 pe cy7, by BD (2 mentions) Pacific blue anti mouse cd4, by BD (2 mentions) Apc cy7 anti mouse cd8, by BD (2 mentions) Apc cy7 anti mouse cd11c, by BD (2 mentions) Af700 anti mouse cd19, by BD (2 mentions) Percp cy5.5 anti mouse cd45r b220, by BD (2 mentions) Lsrii fortessa, by BD (2 mentions) Pe anti mouse cd8α, by BD (1 mentions) Fitc anti mouse cd8, by BD (1 mentions) Apc anti mouse cd4, by BD (1 mentions) Bv605 anti mouse cd4, by BD (1 mentions) Fitc anti mouse cd3ε, by BD (1 mentions) Bv786 anti mouse cd3, by BD (1 mentions) Anti mouse f4 80 pe, by BD (1 mentions) Pe anti mouse cd1d, by BioLegend (1 mentions) Streptavidin apc, by BD (1 mentions) Pe anti mouse foxp3, by BD (1 mentions) Fitc anti mouse cd62l, by BD (1 mentions) Pe anti mouse cd44, by BD (1 mentions) Anti mouse ly6g pe, by BD (1 mentions)

6 protocols using apc anti mouse cd11b

Multiparameter Flow Cytometry Profiling

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For surface staining, cells were first incubated with FcγR blocker (CD16/32), followed by fluorochrome-labeled antibodies (Abs). For intracellular staining, cells were stimulated by phorbol myristate acetate (50 ng/ml) and ionomycin (750 ng/ml) for 5 hrs. After incubation, cells were stained for surface markers first, then fixed by using Foxp3/transcription factor staining set followed by intracellular staining. The specific antibodies and their corresponding isotype controls were purchased from Biolegend (San Diego, CA) and eBioscience (Waltham, MA). The following Abs were used in combinations: PE-Cy7 anti-mouse CD3, Pacific Blue anti-mouse CD4, APC-Cy7 anti-mouse CD8, APC anti-mouse CD11b, APC-Cy7 anti-mouse CD11c, AF700 anti-mouse CD19, Percp-cy5.5 anti-mouse CD45R/B220, FITC anti-mouse CD279 (PD-1) and PEAF610 anti-mouse IFN-γ Abs (BD Pharmingen, San Jose, CA). Flow cytometric analysis were done using an LSRII Fortessa (Becton Dickinson, San Jose, CA), and the data analyzed by using FlowJo software 10.0 (TreeStar, Ashland, OR).

Wang H., Banerjee N., Liang Y., Wang G., Hoffman K.L, & Khan M.F. (2021). Gut microbiome-host interactions in driving environmental pollutant trichloroethene-mediated autoimmunity. Toxicology and applied pharmacology, 424, 115597.

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Comprehensive Immune Cell Profiling

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APC anti-mouse CD4 (clone: RM4-5), BV605 anti-mouse CD4 (clone: GK1.5), FITC anti-mouse CD3ε, PE anti-mouse CD8α, V500 rat anti-mouse B220, FITC anti-mouse CD45R/B220, APC anti-mouse IgD, APC anti-mouse CD21/CD35, PE anti-mouse CD23, FITC anti-mouse CD8, BV786 anti-mouse CD3, PE CF594 anti-mouse CD23, APC Cy7 anti-mouse CD21, FITC anti-mouse Gr1, APC anti-mouse CD11b, PE anti-mouse F4/80, FITC anti-mouse I-A/E, and APC streptavidin were bought from BD bioscience (San Jose, CA). Biotinylated anti-mouse C3 was obtained from Cedarlane (Burlington, Canada), and APC anti-mouse IgG, APC anti-mouse IgM and FITC anti-mouse IgM were purchased from Jackson Immunoresearch (West Grove, PA). PE anti-mouse CD1d and Zombie yellow live dead was purchased from Biolegend (San diego, CA).

Patel S.R., Gibb D.R., Girard-Pierce K., Zhou X., Rodrigues L.C., Arthur C.M., Bennett A.L., Jajosky R.P., Fuller M., Maier C.L., Zerra P.E., Chonat S., Smith N.H., Tormey C.A., Hendrickson J.E, & Stowell S.R. (2018). Marginal Zone B Cells Induce Alloantibody Formation Following RBC Transfusion. Frontiers in Immunology, 9, 2516.

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Multiparametric Immune Cell Analysis

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For surface staining, cells were first incubated with FcγR blocker (CD16/32), followed by fluorochrome-labeled antibodies (Abs). For intracellular staining, cells were stimulated by phorbol myristate acetate (50 ng/ml) and ionomycin (750 ng/ml) for 5 hrs. After incubation, cells were stained for surface markers first, then fixed by using Foxp3/transcription factor staining set followed by intracellular staining. The specific antibodies and their corresponding isotype controls were purchased from Biolegend (San Diego, CA) and eBioscience (Waltham, MA). The following Abs were used in combinations: PE-Cy7 anti-mouse CD3, Pacific Blue anti-mouse CD4, APC-Cy7 anti-mouse CD8, PE anti-mouse CD44, FITC anti-mouse CD62L, APC anti-mouse CD11b, APC-Cy7 anti-mouse CD11c, AF700 anti-mouse CD19, Percp-cy5.5 anti-mouse CD45R/B220, APC anti-mouse IL-17 Abs, and PE anti-mouse Foxp3 (BD Pharmingen, San Jose, CA). Flow cytometric experiments were performed using an LSRII Fortessa (Becton Dickinson, San Jose, CA), and analyzed by using FlowJo software 10.0 (TreeStar, Ashland, OR).

Wang H., Wang G., Liang Y., Du X., Boor P.J., Sun J, & Khan M.F. (2019). Redox regulation of hepatic NLRP3 inflammasome activation and immune dysregulation in Trichloroethene-mediated autoimmunity. Free radical biology & medicine, 143, 223-231.

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MDSC Apoptosis Induction and Inhibition

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Purified MDSCs were incubated at 37°C alone or with E7-specific CD8+ T cells or OT-1-specific CD8+ T cells at a 1:1 ratio for up to 24 hours in 96-well plates. Twenty-four hours later, cells were were stained with APC anti-mouse CD11b, PE anti-mouse Ly6G, FITC anti-mouse Annexin V antibody and 7-AAD (FITC Annexin V Apoptosis Detection Kit, BD Pharmingen, San Diego, CA) and examined by flow cytometry. MDSCs were first gated on CD11b+ and Ly6G^Hi, and then defined as apoptotic by Annexin V+ and 7-AAD-.
To measure antibody-induced apoptosis of MDSCs, isolated MDSCs were cultured for 12 hours with anti-mouse Fas agonistic antibody (2μg/ml, BD Pharmingen, San Diego, CA), or isotype mAb. For the in vitro blocking assay, isolated MDSCs were co-cultured with E7-specific CD8+ T cells or OT-1 specific CD8+ T cells at a 1:1 ratio and then incubated with isotype mAb, Fas-Fas L antagonist (Kp7-6, CALBIOCHEM for 24 hours.

Song L., Yang M.C., Knoff J., Sun Z.Y., Wu T.C, & Hung C.F. (2014). Cancer immunotherapy using a potent immunodominant CTL epitope. Vaccine, 32(46), 6039-6048.

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Immunophenotyping of Mouse Hematopoietic Cells

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mHPCs were collected, washed with cold PBS containing 2% FBS and stained with fluorochrome conjugated monoclonal antibodies anti-mouse CD11b-APC (BD-Bioscience) or anti-mouse Ly-6A/E (Sca-1)-PE (eBioscience, San Diego, CA). Cells were acquired and analyzed on a FACS Canto II flow cytometer (Becton Dickinson, Franklin Lakes, NJ) using the FACS Diva software (BD Biosciences, Bedford, MA).

Barriocanal-Casado E., Cueto-Ureña C., Benabdellah K., Gutiérrez-Guerrero A., Cobo M., Hidalgo-Gutiérrez A., Rodríguez-Sevilla J.J., Martín F, & López L.C. (2016). Gene Therapy Corrects Mitochondrial Dysfunction in Hematopoietic Progenitor Cells and Fibroblasts from Coq9R239X Mice. PLoS ONE, 11(6), e0158344.

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Murine Osteoclast Precursor Identification

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Bone marrow was flushed from 6–8 week old mice using 1X PBS with 2% FBS. Red blood cells were lysed using 1X RBC lysis buffer (Biolegend). Cells were washed twice in 1X PBS with 0.5% BSA, then blocked using rat IgG (TruStain fcX, Biolegend) for 10 minutes on ice. Cells (1 × 10⁶ cells in 100 μl) were then detected with antibodies at 0.2 μg per million cells for 30 minutes on ice protected from light. The antibodies used for flow cytometry to detect osteoclast precursors were the following: anti-mouse CD3-BV421 (BD Biosciences #564008), anti-mouse CD45R-Alexa Fluor488 (BD Biosciences #557669), anti-mouse CD11b-APC (BD Biosciences #553312), anti-mouse CD115-PE (BD Biosciences #565249). Flow cytometry was conducted with a LSR II (BD Biosciences) and data were analyzed using FlowJo software.

Ramaswamy G., Kim H., Zhang D., Lounev V., Wu J.Y., Choi Y., Kaplan F.S., Pignolo R.J, & Shore E.M. (2017). Gsα Controls Cortical Bone Quality by Regulating Osteoclast Differentiation via cAMP/PKA and β-Catenin Pathways. Scientific Reports, 7, 45140.

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