ELISA kits involving hsa-IL-10 (ab46034, Abcam), mmu-IL-10 (ab46103, Abcam), hsa-TNF-α (ab100654, Abcam) and mmu-TNF-α (ab208348, Abcam) were commercially obtained to detect the levels of IL-10 and TNF-α in the supernatant of U87MG cells, peripheral blood of mice and lysate supernatant of mouse xenograft tumors with 10 μL sample added in each well of a 96-well microtiter plate. The OD value was measured by an automatic microplate reader at wavelength of 450 nm with normalization to diluted antibodies in the medium, ranging from 10 to 2000 pg/mL, and standard curves were plotted.
Ab46103
Manufactured by Abcam
Sourced in United States
Ab46103 is a lab equipment product manufactured by Abcam. It serves as a general-purpose tool for various laboratory applications. The product's core function is to provide a reliable and consistent solution for researchers and scientists.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using ab46103
1
ELISA Quantification of Cytokines
2
Quantifying Inflammatory Cytokines in Sepsis
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Mice were euthanized 24 h after CLP, and then plasma and PLF were collected. The concentrations of mice inflammatory cytokines: Tnf, IL-6, IL-1β, and IL-10 were directly quantified by Tnf (#E16ME0004, Enogene), IL-6 (#E16ME0006, Enogene), IL-1β (#E16ME0015, Enogene), and IL-10 (#ab46103, Abcam) ELISA kits.
3
Quantifying Cytokine Levels in Glutamate-Induced Samples
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Cytokine levels were measured using the mouse ultrasensitive ELISA kit assay for IL-1β (ab100704), IL-4 (ab100710), IL-6 (Abcam, ab100712), IL-10 (ab46103), IL-12 p40/70 (100699), TNF-α (100747), and TGF-β (119557). The tissue samples, corresponding to 100 μg of striatal protein (diluted 1:10), were obtained after different times (1, 3, 6, 12, and 24 h) of glutamate administration (Fig. 1 ) and were used for cytokines measurement according to the manufacturer’s protocol (Abcam, MA, USA). Assays were carried out in a colorimetric spectrometer (Shimadzu, RF-5301PC). The results were calculated based on a concentration curve provided by the manufacturer and were expressed as picograms of cytokine per milligrams of protein.
4
Multiplex Cytokine Quantification
5
Serum Cytokine and Autoantibody Analysis
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Shortly after the animals were euthanized, the blood was drawn via cardiac puncture. Blood was left to clot for 30 min at room temperature then centrifuged at 1212× g for 8 min. Serum was isolated, aliquoted and then stored in −80 °C freezer for further analysis later. ELISA was used for the analysis of serum EGF (ab100679, abcam), Anti-SSA/Ro (5710, Alpha Diagnostics), anti-SSB/La (5810, Alpha Diagnostics), and IL-10 (ab46103, abcam).
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