Graphite (282863), H2SO4 (339741), KMnO4 (223468), H2O2 (216763), HCl (H1758), NaNO3 (S5506), 3-aminopropyltriethoxylsilane (APTES) solution (440140), hydrazine solution (35%) (309400), ethanolamine (NH2CH2CH2OH; ≥98%) (E9508), Cr etchant (651826), Au etchant (651818), and myoglobin (MB; M0630) were purchased from Sigma-Aldrich (USA); 5′-amine modified, and 5′-amine/3′-FAM (6-carboxy fluorescein) modified cTnT aptamers (091), where the sequence of the aptamers was 5′-ATACGGGAGCCAACACCAGGACTAACATTATAAGAATTGCGAATAATCATTGGAGAGCAGGTGTGACGGAT-3′, were obtained from OTC Biotech (USA); cTnT (9202–1107) and cTnI (9202–0707) antigens were obtained from AbD-Serotec (USA); normal human serum (S1-100ML) was procured from Merck Millipore (USA); diethylpyrocarbonate (DEPC)-treated water (W2004) and dimethylformamide (DMF) (D1021; 98%) were procured from Biosesang (South Korea); PBSE (P130) was procured from Thermo Scientific (USA); and PBS (pH 7.4, 10×) was procured from Life Technologies (South Korea). DEPC-treated water was used for dilution of the aptamer stock solution, which was stored at −20 °C before use. Deionized (DI) water (18.2 MΩ) was prepared from the water purification system (Millipore).
Normal human serum s1 100ml
Manufactured by Merck Group
Sourced in United States
Normal human serum (S1-100ML) is a laboratory reagent that provides a source of normal human serum. It is intended for use in various in vitro diagnostic procedures and research applications that require a standardized human serum sample.
Automatically generated - may contain errors
2 protocols using normal human serum s1 100ml
1
Aptamer-Based Myoglobin Detection Protocol
2
Electrochemical Aptasensor for Cardiac Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Graphite
(282863), H2SO4 (339741), KMnO4 (223468),
H2O2 (216763), HCl (H1758), NaNO3 (S5506), C2H3O2Cl (C19627), EDC
(3449), BSA (A2153), and myoglobin (Mb; M0630) were procured from
Sigma-Aldrich (USA); PEI (P3143) was purchased from Polyscience, Inc,
(USA); glass wafers (6 in., Pyrex 7740) were procured from Inexus,
Inc., (South Korea); Ag/AgCl ink (RD66) was purchased from BAS Inc.
(Tokyo, Japan); Mb aptamer (089) was obtained from OTC Biotech (USA),
and its sequence was 5′-ATCCGTCACACCTGCTCTTAATTACAGGCAGTTCCACTTAGACAG
ACACACGAATGGTGTTGGCTCCCGTAT-3; cTnT (9202-1107) and cTnI (9202-0707)
antigens were purchased from AbD Serotec (USA); normal human serum
(S1-100ML) was procured from Merck Millipore (USA); NaOH (S1011) and
diethylpyrocarbonate (DEPC)-treated water (W2004) were obtained from
Biosesang (South Korea); and PBS (pH 7.4, 10×) was procured from
Life Technologies (South Korea) and 10-fold-diluted. DEPC-treated
water was used for dilution of the aptamer stock solution, which was
stored at −20 °C before use.
(282863), H2SO4 (339741), KMnO4 (223468),
H2O2 (216763), HCl (H1758), NaNO3 (S5506), C2H3O2Cl (C19627), EDC
(3449), BSA (A2153), and myoglobin (Mb; M0630) were procured from
Sigma-Aldrich (USA); PEI (P3143) was purchased from Polyscience, Inc,
(USA); glass wafers (6 in., Pyrex 7740) were procured from Inexus,
Inc., (South Korea); Ag/AgCl ink (RD66) was purchased from BAS Inc.
(Tokyo, Japan); Mb aptamer (089) was obtained from OTC Biotech (USA),
and its sequence was 5′-ATCCGTCACACCTGCTCTTAATTACAGGCAGTTCCACTTAGACAG
ACACACGAATGGTGTTGGCTCCCGTAT-3; cTnT (9202-1107) and cTnI (9202-0707)
antigens were purchased from AbD Serotec (USA); normal human serum
(S1-100ML) was procured from Merck Millipore (USA); NaOH (S1011) and
diethylpyrocarbonate (DEPC)-treated water (W2004) were obtained from
Biosesang (South Korea); and PBS (pH 7.4, 10×) was procured from
Life Technologies (South Korea) and 10-fold-diluted. DEPC-treated
water was used for dilution of the aptamer stock solution, which was
stored at −20 °C before use.
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