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Anti ly6g antibody

Manufactured by Wuhan Servicebio Technology
Sourced in China

The Anti-Ly6G antibody is a laboratory reagent used for the detection and analysis of Ly6G, a protein marker expressed on the surface of granulocytes, particularly neutrophils. This antibody can be utilized in various immunological techniques, such as flow cytometry, to identify and study the properties of these cell types.

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2 protocols using anti ly6g antibody

1

Immunohistochemistry of Kidney Injury Markers

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Mice kidney samples were incubated with H2O2 before treatment with anti-NGAL antibody (1:100, Abcam, USA), anti-MPO antibody (1:500, Servicebio, China) anti-Ly6G antibody (1:500, Servicebio, China) and anti-F4/80 antibody (1:500, Servicebio, China). The slices were washed by PBS before incubating with a secondary antibody. The positive areas of NGAL and positive cells of MPO, Ly6G and F4/80 were measured under a light microscope and calculated by ImageJ software (version 1.51, USA).
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2

Quantifying Pancreatic Neutrophils

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Paraffin-embedded sections (5 μm) of pancreatic tissue were incubated with a rabbit polyclonal anti-Ly6G antibody (1:500, Servicebio, Wuhan, China) at 4°C overnight. Further experimental steps were performed according to the manufacturer’s instructions for the Streptavidin/Peroxidase Histostain™ Plus Kit (ZSGQ-BIO, Beijing, China). The number of neutrophils was determined by counting Ly6G+ cells per microscopic field (200×).
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