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Eos rebel t1i

Manufactured by Canon
Sourced in Japan

The EOS Rebel T1i is a digital single-lens reflex (DSLR) camera designed and manufactured by Canon. It features a 15.1-megapixel CMOS sensor, DIGIC 4 image processor, and the ability to record full HD 1080p video at 20 frames per second.

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5 protocols using eos rebel t1i

1

Trypan Blue Viability Assay

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Live and UV-inactivated parasites were checked for damage to the cell membrane via the trypan blue dye test. Trypan blue solution (0.4% in PBS, Cellgro) was mixed with purified tachyzoites (1:1) and immediately visualized under brightfield using a Nikon Eclipse E400 microscope. Images were taken with the program EOS Utility 2.8.1.0 (Canon, EOS Rebel T1i camera).
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2

Timed Up and Go Test with Vestibular Stimulation

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During this walking test, the participants were asked to perform a 5-m Timed Up and Go (TUG) test at their preferred walking speed while wearing a pair of accelerometers (Physilog® IV, GaitUp, Switzerland) fixed on the top of their shoes. A total of six iTUG trials were administered of which the first three were without stimuli (“GVS off” trials) and the last three were with vestibular stimulation (“GVS on” trials). Such an order for trials was chosen to minimize any residual effect that vestibular stimulation might have in the subsequent trials. To remove any bias that subjects might have had toward the electric sensation, the study was designed as a single-blind experiment in which the subjects were not aware of the order of “GVS on” and “GVS off” tests. The electrodes were placed on participants’ skin and were connected to the stimulator during the entire data collection session. Sham stimulation was administered during the “GVS off” trials. The participants were told that the stimulus was always on with different degrees of intensity and, therefore, they might or might not feel the current during the trials. The iTUG trials were timed with a stop watch and also recorded with a camera (EOS Rebel T1i, Canon, Japan).
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3

Laser-Enabled Bilayer Actuation Analysis

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The tested samples had their edges taped onto a glass slide which was fixed to an optical mount. The illumination area of a collimated green laser with wavelength of 532 nm (Millennia Pro™, Spectra-Physics, USA) was expanded by a lens to cover the entire sample. The beam waist was measured by the knife-edge method. For actuator displacement analysis the beam waist radius was 4.32 mm. The total output power Ptotal of the laser was measured by an optical power meter (PM100A, Thorlabs GmbH, Germany). The received power (or input power, Pinput) on the sample surface from a Gaussian laser beam was calculated by: Pinput=(1R)w/2w/2l/2l/2I0exp2(x2+y2)r2dxdy where R is the reflectance of the sample surface, r is the beam radius, I0 is the intensity amplitude of the laser calculated by I0=2Ptotal/πr2 for Gaussian beam profile, w and l are the sample width and length, respectively.
The illumination intensity (I) was calculated by: I=Pinput/A where A is the area of the sample.
The deformation of the bilayer during actuation was recorded by a DSLR camera (Canon EOS rebel T1i) at a frame rate of 30 fps and the tip movement was analyzed using Matlab®. The length of the samples (1 cm) was used to calibrate the pixel length in order to calculate the displacement. All the displacement measurements were performed at 20–25 °C and 20–30% RH.
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4

Twitch Ring Assay for Bacterial Motility

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Colonies of cells were picked with a 10 μl pipette tip and stabbed through the agar of a LB 1.5% agar plate and placed at 30 °C for 4 days. After 4 days, the agar was gently removed from the dish and a sufficient volume of 0.5% (w/v) crystal violet in water was added to the plate until the surface covered. After 5 min of staining, the crystal violet solution was removed, and the plate was washed three times with water. The resulting crystal violet stained twitch rings were imaged using a Canon EOS Rebel T1i and measured in FIJI (41 (link), 42 (link), 43 (link)).
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5

Twitching Motility Assay for Bacteria

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Individual colonies of cells of the RWJ collection, as well as PA14 and PAO1 as twitch positive controls, were picked with a 10 μl pipette tip and stabbed through the agar of a LB 1.5% agar plate and placed at 30°C for 4 days. After 4 days, the agar was gently removed from the dish and a sufficient volume of 0.5% (w/v) crystal violet in water was added to the plate until the surface covered. After 5 minutes of staining, the crystal violet solution was removed, and the plate was washed 3 times with water. The resulting crystal violet stained twitch rings were imaged using a Canon EOS Rebel T1i (Lake Success, NY) and measured in FIJI [27 (link)–29 (link)].
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