Purified colchicine

Manufactured by Merck Group

Sourced in Sao Tome and Principe

Purified Colchicine is a laboratory reagent produced by Merck Group. It is a plant-derived compound used in various research applications. The product provides a consistent and high-purity source of colchicine for scientific investigations.

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Lab products found in correlation

Methyl thiazolyl tetrazolium (mtt), by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Streptomycin, by Cell Signaling Technology (1 mentions) Annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit, by BD (1 mentions) Antibodies against bcl 2, by Santa Cruz Biotechnology (1 mentions) Dulbecco s modified eagle s medium dmem, by Cell Signaling Technology (1 mentions) Rpmi 1640, by Cell Signaling Technology (1 mentions) Propidium iodide, by BD (1 mentions) Fetal calf serum (fcs), by GE Healthcare (1 mentions) Fetal bovine serum (fbs), by GE Healthcare (1 mentions) Rpmi 1640 medium, by GE Healthcare (1 mentions) Annexin 5 fitc pi apoptosis detection kit, by Keygen Biotech (1 mentions) Hoechst 33342, by Keygen Biotech (1 mentions)

2 protocols using purified colchicine

Colchicine-Induced Apoptosis Pathway

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Purified Colchicine (99.89%), MTT, and DMSO were purchased from Sigma–Aldrich Co. (St. Louis, MO, U.S.A.). Antibodies against Bcl-2, Bax, cleaved-caspase-3, p53, Rb, phospho Rb (pRb), β-actin and horseradish peroxidase (HRP) secondary conjugated antibodies were obtained from Santa Cruz Biotechnology, Inc., (Santa Cruz, CA, U.S.A.). Dulbecco’s Modified Eagle’s Medium (DMEM), RPMI 1640, fetal bovine serum (FBS), penicillin, and streptomycin were obtained from Cell Signaling Technology (Danvers, MA, U.S.A.). Annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit and propidium iodide (PI) were purchased from BD Bioscience (San Jose, CA, U.S.A.).

Yan L., Huang H., Zhang Y., Yuan X., Yan Z., Cao C, & Luo X. (2020). Involvement of p53-dependent apoptosis signal in antitumor effect of Colchicine on human papilloma virus (HPV)-positive human cervical cancer cells. Bioscience Reports, 40(3), BSR20194065.

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Colchicine-Induced Apoptosis Mechanism

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Purified Colchicine (99.89%, Figure 1), MTT, and DMSO was purchased from Sigma–Aldrich Co. (St. Louis, MO, U.S.A.). Hoechst 33342 and Annexin V-FITC/PI Apoptosis Detection Kit were obtained from KeyGen Biotech (Nanjing, China). Antibodies for bax, bcl-2, cleaved caspase-3, β-actin, and horseradish peroxidase (HRP)–conjugated goat anti-rat secondary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Inc., Dallas, TX, U.S.A.). Rosewell Park Memorial Institute (RPMI) 1640 medium, FCS, antibiotics and FBS were supplied by GE Healthcare Life Sciences (Logan, UT, U.S.A.). All other chemicals were of analytical grade.

Zhang T., Chen W., Jiang X., Liu L., Wei K., Du H., Wang H, & Li J. (2019). Anticancer effects and underlying mechanism of Colchicine on human gastric cancer cell lines in vitro and in vivo. Bioscience Reports, 39(1), BSR20181802.

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