Ezchrom elite hplc system

Manufactured by Hitachi

The EZChrom Elite HPLC system is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a modular design that allows for customization and scalability to meet various laboratory requirements. The system provides precise control of fluid flow, temperature, and other parameters to ensure reliable and reproducible results.

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Lab products found in correlation

Nmr spectrometer, by Bruker (1 mentions) Delta pak c 18, by Waters Corporation (1 mentions) Eurospher 2 100 5 c18 column, by Knauer (1 mentions)

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HPLC system (77 citations) EZChrom Elite (14 citations)

2 protocols using ezchrom elite hplc system

Purification and Characterization of Organic Compounds

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Example 13

All the organic solvents and reagents were purchased from Sigma-Aldrich Corporation and VWR Scientific and used without further purification. The HPLC system consists of a Hitachi EZChrom Elite HPLC system with a L2450 diode array as a detector. The purification system employed a mobile phase system involving 20 mM of ammonium acetate buffer with a flow rate of 2.0 mL/min and a Waters Delta PAK C₁₈(15 μm, 300×7.8 mm) column. MALDI-mass spectra were obtained from Proteomics Resource center at Rockefeller University with using the PerSeptive Voyager DERP MALDI-TOF Mass Spectrometer. HRMS spectra were obtained from the Hunter College Mass Spectrometry Facility. ¹H and ¹³C NMR spectra were recorded on Bruker NMR spectrometer operating at 400 MHz (DPX Avance), respectively. ¹H and ¹³C chemical shifts are expressed in ppm with respect to the standard deuterium solvent peaks.

US11633414B2. Nicotinoyl riboside compositions and methods of use (2023-04-25). Cornell University [US]. Inventors: Anthony A. Sauve [US], Tianle Yang Redanz [US].

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HPLC Analysis of P. aeruginosa AQ Levels

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AQ levels in P. aeruginosa cultures were determined with a Hitachi EZchrom Elite HPLC system. Culture samples (500 µL each) were extracted three times with 500 µL ethyl acetate (acidified with 1 mL L⁻¹ acetic acid), dried to completion, re-dissolved in acidified (0.1% (w/v) citric acid) methanol, and separated by HPLC on a Eurospher II 100-5 C18 column (Knauer) at 35 °C using a linear gradient (within 40 min) from 60% methanol in water with 0.1% (w/v) citric acid to 100% methanol with 0.1% (w/v) citric acid at a flow rate of 0.5 mL min⁻¹. A diode array detector (L-2450 LaChrome Elite, Merck Hitachi) was used to record the light absorption spectra of eluted compounds. PQS, HHQ, and HQNO were used as reference compounds for calibration.

Arranz San Martín A., Vogel J., Wullich S.C., Quax W.J, & Fetzner S. (2022). Enzyme-Mediated Quenching of the Pseudomonas Quinolone Signal (PQS): A Comparison between Naturally Occurring and Engineered PQS-Cleaving Dioxygenases. Biomolecules, 12(2), 170.

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