Fasting maternal venous blood samples were obtained on the day of electronic impedance cardiography monitoring. Samples were centrifuged, and supernatants were stored at − 80 °C. TSH was measured using an Elecsys-TSH kit (Roche Diagnostics, Mannheim, Germany, RRID:AB_2756377). FT4 was measured using an FT4 Flex reagent (Siemens, Berlin, Germany, RRID:AB_2801666). Thyroid peroxidase antibody was measured with an E-Anti-TPO kit (Roche Diagnostics, Mannheim, Germany, RRID:AB_10698637) and was graded as positive when greater than 60 IU/ml. The normal reference for TSH in the third trimester was 0.3 to 3.5 mIU/L, as recommended [21 (link)].
Elecsys tsh kit
Manufactured by Roche
The Elecsys TSH kit is a laboratory test used to measure the levels of thyroid-stimulating hormone (TSH) in a patient's blood sample. TSH is a hormone produced by the pituitary gland that regulates the function of the thyroid gland. The Elecsys TSH kit provides a quantitative measurement of TSH levels, which is important for the diagnosis and management of thyroid disorders.
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4 protocols using elecsys tsh kit
1
Maternal Thyroid Function Monitoring
2
Thyroid Function and Autoantibody Evaluation
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Thyroid function tests which consisted of serum free T3 (fT3), free T4 (fT4), and TSH levels were performed using electrochemiluminescent immunoassay (Elecsys FT3 II kit, Elecsys FT4 kit, and Elecsys TSH kit, respectively; Roche Diagnostics, Mannheim, Germany); the reference ranges were 2.33–4.00 pg/mL, 0.880–1.620 ng/dL, and 0.500–5.000 μIU/mL, respectively. Levels of anti-thyroperoxidase antibodies (TPOAbs) and anti-thyroglobulin antibodies (TgAbs) were also measured using electrochemiluminescent immunoassay (Elecsys Anti-TPO kit and Elecsys Anti-Tg kit, respectively; Roche Diagnostics). Thyroid uptake of FDG-PET was defined as diffusely increased FDG uptake in the thyroid gland.
3
Thyroid Function Biomarker Measurement
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Serum levels of free T3 (fT3), free T4 (fT4), and TSH were measured with electrochemiluminescence immunoassays. During the period analyzed in the retrospective study, Elecsys kits were used (Elecsys FT3 II kit, Elecsys FT4 kit, and Elecsys TSH kit; Roche Diagnostics, Mannheim, Germany), and the reference ranges were 2.33-4.00 pg/mL, 0.880-1.620 ng/dL, and 0.500-5.000 μIU/mL, respectively. In the case series, AIA kits were used (AIA-PACK CL FT3 kit, AIA-PACK CL FT4 kit, and AIA-PACK CL TSH kit; Tosoh, Tokyo, Japan), and the reference ranges were 2.06-3.17 pg/mL, 0.880-1.620 ng/dL, and 0.500-5.000 μIU/mL, respectively. TgAb, TPOAb, and anti-TSH receptor antibody (TRAb) were measured using Elecsys kits (Roche Diagnostics), with reference ranges of <28 IU/L, <16 IU/L, and <2.0 IU/L, respectively. Measurement of thyroid stimulating antibody (TSAb) titers was performed using a TSAb enzyme immunoassay kit (Yamasa Corp., Chiba, Japan), with a cut-off level of 120%.
4
Comprehensive Thyroid Function Evaluation
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Serum levels of free T3 (fT3), free T4 (fT4), TSH, and thyroglobulin (Tg) were measured with electrochemiluminescence immunoassays (Elecsys FT3 II kit, Elecsys FT4 kit, Elecsys TSH kit, and Elecsys Tg II kit, respectively; Roche Diagnostics, Mannheim, Germany). The reference ranges were 2.33-4.00 pg/mL, 0.880-1.620 ng/dL, 0.500-5.000 μIU/mL, and <33.7 ng/mL respectively. Anti-thyroid peroxidase antibody (TPOAb), anti-thyroglobulin antibody (TgAb), and anti-TSH receptor antibody (TRAb) were measured using Elecsys kits (Roche Diagnostics). The reference ranges were <16 IU/L, <28 IU/L, and <2.0 IU/L, respectively. Measurement of thyroid stimulating antibody (TSAb) titers was performed using a TSAb enzyme immunoassay kit (Yamasa Corp., Chiba, Japan); the cut-off level was 120%.
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