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Alexafluo 647 anti mouse secondary antibodies for immunofluorescence

Manufactured by LI COR

Alexafluo-647 anti-mouse secondary antibodies are fluorescently labeled antibodies used for immunofluorescence applications. These antibodies specifically bind to mouse primary antibodies, allowing for the detection and visualization of target proteins or structures in cell or tissue samples.

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2 protocols using alexafluo 647 anti mouse secondary antibodies for immunofluorescence

1

Immunofluorescence Antibody Validation

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The following primary antibodies were used: rabbit polyclonal hnRNP M (Abcam, #177957), rabbit polyclonal Histone 3 (Abcam, #1791), mouse monoclonal Beta-Actin (Abcam, #6276), mouse monoclonal hnRNP L (Abcam, #6106–100), rabbit polyclonal Beta-Tubulin (Abcam, #179513), mouse monoclonal hnRNP U (Santa-Cruz, sc-32315), DAPI nuclear staining (Thermo Fisher), and mouse monoclonal ANTI-FLAG M2 antibody (Sigma-Aldrich, F3165). Secondary antibodies used were asfollows: IR Dye CW 680 goat anti-rabbit, IR Dye CW800 goat anti-mouse (LI-COR), Alexfluor-488 anti-rabbit and Alexafluo-647 anti-mouse secondary antibodies for immunofluorescence (LI-COR).
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2

Immunofluorescence Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used: rabbit polyclonal hnRNP M (Abcam, #177957), rabbit polyclonal Histone 3 (Abcam, #1791), mouse monoclonal Beta-Actin (Abcam, #6276), mouse monoclonal hnRNP L (Abcam, #6106–100), rabbit polyclonal Beta-Tubulin (Abcam, #179513), mouse monoclonal hnRNP U (Santa-Cruz, sc-32315), DAPI nuclear staining (Thermo Fisher), and mouse monoclonal ANTI-FLAG M2 antibody (Sigma-Aldrich, F3165). Secondary antibodies used were asfollows: IR Dye CW 680 goat anti-rabbit, IR Dye CW800 goat anti-mouse (LI-COR), Alexfluor-488 anti-rabbit and Alexafluo-647 anti-mouse secondary antibodies for immunofluorescence (LI-COR).
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