The expressions of pluripotency-associated markers were analyzed by flow cytometry. Feeder-free cultured iPSCs were passaged by TrypLE (Life Technologies, Waltham, MA, USA) to be prepared as a suspension. The cells were stained with antibodies for SSEA4, Tra1-81, and Oct3/4 (BD Biosciences Pharmingen, San Diego, CA, USA).
Antibodies for ssea4
Manufactured by BD
Sourced in United States, China
Antibodies for SSEA4 are laboratory reagents used to detect and measure the presence of the SSEA4 (Stage-Specific Embryonic Antigen 4) protein in biological samples. SSEA4 is a cell surface marker that is commonly expressed in undifferentiated stem cells. These antibodies can be used in various research techniques, such as flow cytometry, immunocytochemistry, and Western blotting, to investigate the properties and behavior of stem cells.
Automatically generated - may contain errors
2 protocols using antibodies for ssea4
1
Pluripotency Marker Expression Analysis
2
Pluripotency Validation via Flow Cytometry
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Flow cytometric analysis was used for pluripotency validation [20] . GC-ADO2-iPSCs were dissociated with Solase dissociation buffer (no.
RP01021, Nuwacell Biotechnologies Co., Ltd., Hefei, Anhui, China) and incubated with the antibodies for SSEA-4 (no. 560128, BD Pharmingen, San Diego, CA, USA) or TRA-1-81 (no. 560161, BD Pharmingen, San Diego, CA, USA) at 4 ℃ for 20 min in dark. Samples were then analyzed using a FACScan flow cytometry (BD Biosciences, San Diego, CA, USA), and data were analyzed using the FlowJo Software (version 7.6.5, FlowJo LLC, Ashland, OR, USA).
RP01021, Nuwacell Biotechnologies Co., Ltd., Hefei, Anhui, China) and incubated with the antibodies for SSEA-4 (no. 560128, BD Pharmingen, San Diego, CA, USA) or TRA-1-81 (no. 560161, BD Pharmingen, San Diego, CA, USA) at 4 ℃ for 20 min in dark. Samples were then analyzed using a FACScan flow cytometry (BD Biosciences, San Diego, CA, USA), and data were analyzed using the FlowJo Software (version 7.6.5, FlowJo LLC, Ashland, OR, USA).
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