Animals were anaesthetised with isoflurane (1.5 % v/v), and the skull was exposed through a midline incision. A cranial window was created and closed by gluing a glass coverslip onto the skull and adhering the skin to the coverslip rim. For imaging, mice were anaesthetised with isoflurane (1 % v/v) and fixed within a stereotaxic frame (Narishige). Brain perfusion was determined through a closed cranial window in anaesthetised mice using PeriCam PSI Laser Speckle Imager and PIMsoft software (Perimed). PcomA development was determined by transcardial perfusion with India ink (Pelikan; 10 % in water containing 10 % gelatine), followed by decapitation and fixation in 4 % paraformaldehype (PFA). PcomA was scored as: 0, absent; 1, hypoplastic; 2, truncal. A single PcomA score was calculated by averaging left and right scores.
Pimsoft
Manufactured by Perimed
Sourced in Sweden
PIMSoft is a software application developed by Perimed for data acquisition and analysis. The software is designed to work with Perimed's range of medical and research equipment, enabling users to collect, manage, and analyze data from various measurement modalities.
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Lab products found in correlation
23 protocols using pimsoft
1
Imaging and Scoring of PcomA Development
2
Quantifying Cutaneous Blood Perfusion by LSCI
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Cutaneous blood perfusion quantification was performed with laser speckle contrast imaging (LSCI; PeriCam PSI System, Perimed AB, Järfälla, Sweden), as previously described.30 In short, assessments were performed in a temperature‐controlled room (22°C) after acclimatization of the subjects. LSCI recordings of the target area on the left and right ventral forearms were captured with the use of dedicated software (PimSoft, Perimed AB). Circular regions of interest at the intradermal injection sites were defined and cutaneous blood perfusion (indicated as basal flow) was quantitatively assessed and expressed in arbitrary units (AUs). The homogeneity of cutaneous blood perfusion in the region of interest (indicated as flare), expressed as values that are +1 standard deviation (SD) from the mean basal flow within the region, was also quantitatively assessed and expressed in AUs. Illustrations of cutaneous blood perfusion measured with LSCI are depicted in Figure 1
3
Cutaneous Microvascular Flow Dynamics
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A laser speckle contrast imaging system with a laser wavelength of 785 nm (PeriCam PSI system, Perimed, Järfälla, Sweden) coupled to iontophoresis of acetylcholine and sodium nitroprusside will measure non-invasively real-time cutaneous microvascular flow changes in the forearm [42 (link)]. For the post-occlusive reactive hyperemia (PORH) test, arterial occlusion will be performed with suprasystolic pressure (50 mmHg above the systolic arterial pressure) using a sphygmomanometer applied to the arm of the subject over 3 min. Peak skin flow will be measured after pressure release.
Images will be analyzed using the manufacturer’s software (PIMSoft, Perimed, Järfälla, Sweden). The measurements of skin blood flow will be divided by the mean arterial pressure to yield the cutaneous vascular conductance (CVC) in arbitrary perfusion units (APU)/mmHg, to avoid interference of blood pressure levels on calculation of microvascular flow.
Images will be analyzed using the manufacturer’s software (PIMSoft, Perimed, Järfälla, Sweden). The measurements of skin blood flow will be divided by the mean arterial pressure to yield the cutaneous vascular conductance (CVC) in arbitrary perfusion units (APU)/mmHg, to avoid interference of blood pressure levels on calculation of microvascular flow.
4
Vascular Response to Cold Exposure
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All vascular measurements were performed 1 or 2 days prior to the surgery (baseline) and 1 month after, in a temperature-controlled room by one vascular technician.
The cooling and recovery procedure was performed on both hands, as described previously [9 (link)]. In short, photo-electric plethysmography sensors were placed on all fingertips to assess blood flow. Both hands were submerged in water up till the radio carpal joint. Every 4 min, the water was cooled by 3°C, from 33°C to 6°C, or until the patient could not tolerate the pain any more. After cooling, the blood flow was assessed every minute during a 10-min recovery period.
LASCA was performed to measure peripheral blood perfusion, at room temperature (23°C), on both hands using a PeriCam PSI System (PeriMed, Jarfalla, Sweden), and data acquisition and analysis were performed making use of PIMSoft (PeriMed). Perfusion was processed as numerical values in perfusion units and colour-coded images. A total of 1 min was recorded per hand, of which a stable period of 10 s was assessed. Two regions of interest were selected: ROI1 entailed the index, middle and ring finger distal from the DIP joint, and ROI2 the dorsum of the hand. The perfusion gradient was calculated by subtracting ROI2 from ROI1, as described previously [10 (link)].
The cooling and recovery procedure was performed on both hands, as described previously [9 (link)]. In short, photo-electric plethysmography sensors were placed on all fingertips to assess blood flow. Both hands were submerged in water up till the radio carpal joint. Every 4 min, the water was cooled by 3°C, from 33°C to 6°C, or until the patient could not tolerate the pain any more. After cooling, the blood flow was assessed every minute during a 10-min recovery period.
LASCA was performed to measure peripheral blood perfusion, at room temperature (23°C), on both hands using a PeriCam PSI System (PeriMed, Jarfalla, Sweden), and data acquisition and analysis were performed making use of PIMSoft (PeriMed). Perfusion was processed as numerical values in perfusion units and colour-coded images. A total of 1 min was recorded per hand, of which a stable period of 10 s was assessed. Two regions of interest were selected: ROI1 entailed the index, middle and ring finger distal from the DIP joint, and ROI2 the dorsum of the hand. The perfusion gradient was calculated by subtracting ROI2 from ROI1, as described previously [10 (link)].
5
Investigating Cerebral Microcirculation in Ischemic Stroke
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To analyze the effect of low and high dose of TAK-063 on cerebral microcirculation, LSI was performed as described previously [20, (link)19] (link). Brie y, C57BL/6 mice were subjected to 90 min MCAO as described above followed by the oral delivery of vehicle, low dose TAK-063 or high dose TAK-063 at the onset of reperfusion. Thereafter, cerebral microcirculation was recorded by Pericam PSI System (Perimed) for 90 min. To analyze CBF changes in the ischemic core and periphery, regions of interest (ROI) covering 1.0 mm x 5.5 mm (in lateral and rostrocaudal direction, respectively) were de ned 1.5 and 2.5 mm lateral and 0.5 mm posterior to the bregma, in which mean CBF was calculated using a blood perfusion imaging software (PIMSoft; Perimed) [19] (link). Regional CBF was recorded throughout the 90 min observation period. From the measurements obtained, relative CBF changes (in %) at the end of the observation period compared to the beginning of the observation period were calculated.
6
Laser Speckle Contrast Analysis of Penile Perfusion
7
Quantifying Cutaneous Blood Perfusion
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Cutaneous blood perfusion quantification was performed with laser speckle contrast imaging (LSCI; PeriCam PSI System, Perimed AB, Järfälla, Sweden) as previously described (15 (link)). In short, assessments were performed in a temperature-controlled room (22°C) after acclimatization of the subjects. LSCI recordings of the target area on the left and right ventral forearms were captured with the use of dedicated software (PimSoft, Perimed AB, Järfälla, Sweden). Circular regions of interest at the intradermal injection sites were defined and cutaneous blood perfusion (indicated as basal flow) was quantitatively assessed and expressed in arbitrary units (AUs). The homogeneity of cutaneous blood perfusion in the region of interest (indicated as flare), expressed as values that are +1 standard deviation (SD) from the mean basal flow within the region, was also quantitatively assessed and expressed in AUs.
8
In Vivo Laser Speckle Contrast Imaging in Stroke Mice
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LSCI for stroke animals was performed similar to as reported earlier by us for laser Doppler imaging in experimental stroke [12 (link),13 (link)], with certain suitable modifications as described below. Mice were anesthetized using isofluorane, body temperature was maintained at 37 ± 0.5°C, the skull was shaved, exposed by a midline skin incision and cleaned. Perfusion images were acquired using PeriCam high resolution LSCI (PSI system, Perimed) with a 70 mW built-in laser diode for illumination and 1388 × 1038 pixels CCD camera installed 10 cm above the skull (speed 19 Hz, and exposure time 6 mSec). Acquired images were analyzed for dynamic changes in CBF using PIMSoft (Perimed). Since anesthesia and stroke procedure affects the cerebral perfusion of uninjured stroke side too, the absolute value from the ipsilateral side was normalized with the value from contralateral side and calculated as percent change as reported by us for laser Doppler imaging [12 (link),13 (link)].
9
Evaluating rTMS Effects on Cerebral Microcirculation
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To evaluate the effect of rTMS on microcirculation, LSI was carried out as described previously (Beker et al., 2015 (link)) with minor modifications. Briefly, mice were exposed to 90 min MCAo as described above. Starting 1 min after reperfusion, real-time CBF changes were recorded by Pericam PSI System (Perimed). To evaluate the CBF changes in the ischemic core and ischemic penumbra regions of interest (ROI) covering 1.0 mm × 5.5 mm (in lateral and rostrocaudal direction, respectively) were defined 0.5 and 1.5 lateral and 0.5 mm posterior to the bregma, in which mean CBF was calculated using a blood perfusion imaging software (PIMSoft; Perimed). Regional CBF was recorded throughout the 60 min observation period after rTMS treatment. From the measurements obtained, relative CBF changes (in %) at the end of the observation period which is compared with the values of before rTMS treatment after MCAo.
10
Ischemic Limb Blood Flow Monitoring
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Blood flow measurements were performed using a small-animal blood flow imaging perfusion machine. At 0, 3, 7, 14, 21 and 28 days after surgery, blood flow was measured by PeriCamPS I (Perimed) in a constant temperature, humidity and sound insulation environment (room temperature of 24° C, no excessive light and noise). The imaging data were analysed using PIMSoft (Perimed) software [24 (link)], and the ratio of ischemic limb blood flow to normal limb blood flow was used as the index of ischemic limb blood flow recovery.
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