Milliplex human cardiovascular disease panels

Manufactured by Merck Group

Sourced in United States

The Milliplex Human Cardiovascular Disease panels are a set of multiplex assays designed to measure a variety of cardiovascular disease-related analytes in human biological samples. The panels utilize the Luminex xMAP technology to enable simultaneous quantification of multiple analytes from a single sample. The core function of these panels is to provide a comprehensive and efficient way to analyze cardiovascular biomarkers in research and clinical settings.

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Lab products found in correlation

Quant it picogreen dsdna assay kit, by Thermo Fisher Scientific (1 mentions) Victor3 plate reader, by PerkinElmer (1 mentions) Bio plex manager 6, by Bio-Rad (1 mentions) Luminex 200 instrument, by R&D Systems (1 mentions)

7 protocols using milliplex human cardiovascular disease panels

Cardiovascular Disease Biomarkers Profiling

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Testing was conducted using Milliplex Human Cardiovascular Disease panels (EMD Millipore, USA) as outlined in the manufacturer’s protocols. Soluble biomarkers assessed included MMP-9, tPAI-1, hsCRP, IL-6, IL-8, IL-10, TNF-α, soluble E-selectin (sE-selectin), soluble vascular cell adhesion molecule-1 (sVCAM-1), soluble intercellular cell adhesion molecule-1 (sICAM-1), monocyte chemoattractant protein-1 (MCP-1), and interferon-gamma (IFN-γ).

Chow D.C., Kagihara J.M., Zhang G., Souza S.A., Hodis H.N., Li Y., Mitchell B.I., Nakamoto B.K., Kallianpur K.J., Keating S.M., Norris P.J., Kohorn L.B., Ndhlovu L.C, & Shikuma C.M. (2016). Non-Classical Monocytes Predict Progression of Carotid Artery Bifurcation Intima-Media Thickness in HIV-infected Individuals on Stable Antiretroviral Therapy. HIV clinical trials, 17(3), 114-122.

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Quantification of Circulating cfDNA and NET Markers

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Circulating cfDNA in the plasma was quantified using the Quant-iT™ PicoGreen™ dsDNA Assay-Kit (ThermoScientific, P11496) according to the manufacturer's instructions. The fluorescence was read in a VICTOR3 plate reader (Perkin Elmer) by fluorescence 485 nm/535 nm, 1.0 s protocol. The concentration of the circulating cfDNA was calculated based on the standard provided by the kit. CitH3 was quantified in plasma using the enzyme-linked immunosorbent assay according to the manufacturer’s instructions (Cayman, 501620). We used previously generated plasma cytokine data to assess associations of circulating NET markers and LDG parameters with soluble biomarkers in PLWH. List of cytokines in Table 2 was measured via a MILLIPLEX^® Human Cardiovascular Disease panels (EMD Millipore) according to the manufacturers protocol.

Park J., Dean L.S., Heckl J., Gangcuangco L.M., Pedro T.K., Tallquist M.D., Seto T.B., Shiramizu B., Chow D.C, & Shikuma C.M. (2023). Low-density granulocytes display immature cells with enhanced NET formation in people living with HIV. Scientific Reports, 13, 13282.

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Multiplex Biomarker Profiling of Cardiovascular Disease

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Testing was conducted using Milliplex Human Cardiovascular Disease panels (EMD Millipore, USA) as outlined in the manufacturer’s protocols. Soluble biomarkers assessed included MMP-9, MPO, tPAI-1, CRP, SAA, SAP, IL-1β, IL-6, IL-8, IL-10, TNF-α, soluble E-selectin (sE-selectin), soluble vascular cell adhesion molecule-1 (sVCAM-1), soluble intercellular cell adhesion molecule-1 (sICAM-1), monocyte chemoattractant protein-1 (MCP-1), vascular endothelial growth factor (VEGF), interferon-gamma (IFN-γ), and N-terminal pro-brain natriuretic peptide (NT-proBNP).

Shikuma C.M., Chow D.C., Gangcuangco L.M., Zhang G., Keating S.M., Norris P.J., Seto T.B., Parikh N., Kallianpur K.J., Nakamoto B.K., Nagamine L.S., Ndhlovu L.C, & Barbour J.D. (2014). Monocytes Expand with Immune Dysregulation and Is Associated with Insulin Resistance in Older Individuals with Chronic HIV. PLoS ONE, 9(2), e90330.

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Multiplex Biomarker Analysis of HIV Patients

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Aliquots of plasma from 74 HIV-infected individuals on cART were used for multiplex biomarker analysis using Milliplex human cardiovascular disease panels (EMD Millipore, USA). Soluble biomarkers assessed included sE-selectin, sVCAM-1, sICAM-1, matrix metalloprotease 9 (MMP-9), myeloperoxidase (MPO), plasminogen activator inhibitor-1 (tPAI-1), C-reactive protein (CRP), serum amyloid A (SAA), serum amyloid P (SAP), monocyte chemoattractant protein-1 (MCP-1), vascular endothelial growth factor (VEGF), IL-1β, IL-6, IL-8, IL-10, tumor necrosis factor alpha (TNF-α), and gamma interferon (IFN-γ). Standards and samples were measured in duplicate. Samples were acquired on a Luminex 200 instrument (R&D Systems, Inc.). Bio-Plex Manager 6.1 software (Bio-Rad Laboratories, Inc.) was used for data processing.

Marques de Menezes E.G., Deng X., Liu J., Bowler S.A., Shikuma C.M., Stone M., Hunt P.W., Ndhlovu L.C, & Norris P.J. (2022). Plasma CD16+ Extracellular Vesicles Associate with Carotid Artery Intima-Media Thickness in HIV+ Adults on Combination Antiretroviral Therapy. mBio, 13(3), e03005-21.

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Cytokine Profiling in HIV+ Individuals

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Blood was separated within 30 minutes of the blood draw and plasma aliquots were cryopreserved until used for cytokine/chemokine assessments in a subset of the HIV+ individuals. Testing was conducted using Milliplex Human Cardiovascular Disease panels (EMD Millipore, USA) as outlined in the manufacturer’s protocols. The soluble biomarkers assessed were IL-6, IL-8, IL-10, and TNF-α.

McIntosh R.C., Paul R., Ndhlovu L.C., Hidalgo M., Lobo J.D., Walker M., Shikuma C.M, & Kallianpur K.J. (2018). Resting-State Connectivity and Spontaneous Neural Activity Predict Depressive Symptomology and Peripheral Inflammation in HIV. Journal of neurovirology, 24(5), 616-628.

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Cardiovascular Biomarker Profiling

Cited 1 time

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The plasma soluble biomarkers, matrix metallopeptidase-9, myeloperoxidase, tissue plasminogen activator inhibitor-1, C-reactive protein, serum amyloid A, serum amyloid P, interleukin (IL)-1β, IL-6, IL-8, IL-10, tumor necrosis factor (TNF)-α, soluble E-selectin, soluble vascular cell adhesion molecule-1, soluble intercellular cell adhesion molecule-1 (sICAM-1), monocyte chemoattractant protein-1 (MCP-1), vascular endothelial growth factor, interferon-gamma (IFN-γ), and N-terminal pro-brain natriuretic peptide were measured by Milliplex Human Cardiovascular Disease panels (EMD Millipore, Temecula, CA) as outlined in the manufacturer's protocols as previously described (40 (link)).

D'Antoni M.L., Kallianpur K.J., Premeaux T.A., Corley M.J., Fujita T., Laws E.I., Ogata-Arakaki D., Chow D.C., Khadka V.S., Shikuma C.M, & Ndhlovu L.C. (2019). Lower Interferon Regulatory Factor-8 Expression in Peripheral Myeloid Cells Tracks With Adverse Central Nervous System Outcomes in Treated HIV Infection. Frontiers in Immunology, 10, 2789.

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Measurement of Cardiovascular Biomarkers and Dysfunction

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Fasting blood samples were collected (nothing by mouth for 12 hours except water), stored in EDTA tubes, frozen at −140⁰C and forwarded to LipoScience Inc. (Raleigh, NC). Chemiluminescent immunoassay (DiaSorin) was used to measure serum 25(OH)D levels.
Milliplex Human Cardiovascular Disease panels (EMD Millipore) were used to measure the following plasma soluble biomarkers: C-reactive protein (CRP), interferon-gamma (IFN-γ), interleukin-1beta (IL-1β), IL-6, IL-8, IL-10, matrix metallopeptidase-9 (MMP-9), monocyte chemoattractant protein-1 (MCP-1), myeloperoxidase (MPO), plasminogen activator inhibitor type 1 (PAI-1), serum amyloid A (SAA), serum amyloid P component (SAP), soluble E-selectin (sE-selectin), soluble vascular cell adhesion molecule-1 (sVCAM-1), soluble intercellular adhesion molecule-1 (sICAM-1), tumor necrosis factor-alpha (TNF-α), and vascular endothelial growth factor (VEGF).
Three different markers of arterial dysfunction were available in the database: carotid intima media thickness (cIMT), flow-mediated dilatation (FMD), and coronary artery calcium (CAC). Methods utilized to obtain cIMT, FMD, and CAC have been previously published.^[5] (link)

Gangcuangco L.M., Kohorn L.B., Chow D.C., Keating S.M., Norris P.J., Nagamine L.S., Ndhlovu L.C., Souza S.A., Kallianpur K.J, & Shikuma C.M. (2016). High 25-hydroxyvitamin D is associated with unexpectedly high plasma inflammatory markers in HIV patients on antiretroviral therapy. Medicine, 95(43), e5270.

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