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2 protocols using anti corest

1

Immunoprecipitation and Western Blotting

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For immunoprecipitation (IP), cells were lysed in Triton lysis buffer supplemented with protein inhibitor cocktails (Thermo Fisher Scientific), followed by a brief sonication, and then lysates were immunoprecipitated with anti-E2F1 (Abcam), anti-LSD1 (Abcam), or anti-CoREST (Abcam) antibodies. For immunoblotting, proteins were separated on 4–15% SDS gradient gels (Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad), and then probed with primary antibodies, including anti-Rb (Cell Signaling), anti-tubulin (Abcam), anti-LSD1 (Abcam), anti-CoREST (Abcam), anti-E2F1 (Cell Signaling), anti-GAPDH (Abcam), anti-V5 (Abcam), anti-H3 (Abcam) and anti-FOXA1 (Abcam).
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2

Immunoprecipitation and Western Blotting

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunoprecipitation (IP), cells were lysed in Triton lysis buffer supplemented with protein inhibitor cocktails (Thermo Fisher Scientific), followed by a brief sonication, and then lysates were immunoprecipitated with anti-E2F1 (Abcam), anti-LSD1 (Abcam), or anti-CoREST (Abcam) antibodies. For immunoblotting, proteins were separated on 4–15% SDS gradient gels (Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad), and then probed with primary antibodies, including anti-Rb (Cell Signaling), anti-tubulin (Abcam), anti-LSD1 (Abcam), anti-CoREST (Abcam), anti-E2F1 (Cell Signaling), anti-GAPDH (Abcam), anti-V5 (Abcam), anti-H3 (Abcam) and anti-FOXA1 (Abcam).
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