Ab210574

Manufactured by Abcam

Sourced in United States

Ab210574 is a laboratory product from Abcam. It is a recombinant protein.

Automatically generated - may contain errors

Lab products found in correlation

Rab0477, by Merck Group (1 mentions) Rab0308, by Merck Group (1 mentions) Rab0274, by Merck Group (1 mentions) Kge001, by R&D Systems (1 mentions) Ab176648, by Abcam (1 mentions)

2 protocols using ab210574

Evaluating Inflammatory Markers in VSMCs

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Two hours after transfection, Ang II or PBS was added into medium to pretreat the transfected VSMCs for 24 hours. The cell supernatant or separated serum of mice was collected to determine levels of TNF-α (RAB0477; Sigma-Aldrich Chemical Company, St Louis, MO, USA), COX-2 (ab210574, Abcam Inc., Cambridge, MA, USA), IL-6 (RAB0308; Sigma-Aldrich Chemical Company, St Louis, MO, USA), and IL-1β (RAB0274; Sigma-Aldrich Chemical Company, St Louis, MO, USA) by ELISA. Because NO would immediately transform into NO2- and further transform into NO3-, Griess reagent was used to measure the content of NO (KGE001, R&D Systems, Minneapolis, MN, USA) based on the reaction between nitrite and nitrate. Three replicates were set for each group.

Lin H., You B., Lin X., Wang X., Zhou D., Chen Z., Chen Y, & Wang R. (2020). Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition. Aging (Albany NY), 12(13), 12684-12702.

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Measuring Inflammatory Cytokine Levels in RAW 264.7 Cells

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The COX‐2 and NF‐κB level was measured in the cell lysate of RAW 264.7 following the manufacturer's instructions using 96well plates pre‐coated with target‐specific immobilised antibody (Abcam, abID# ab210574 and abID# ab176648, respectively). The OD values and standard reference curve determined the pro‐inflammatory cytokine levels (ng mL⁻¹). The fold change in cytokine expression was calculated using Equation (5), and the results are presented as mean±SD from two experiments.

F o l d c h a n g e = \frac{N F - K B o r C O X - 2 l e v e l i n t r e a t m e n t c e l l s}{N F - K B o r C O X - 2 l e v e l i n L P S E c c e l l s}

Mak K., Shiming Z., Epemolu O., Dinkova‐Kostova A.T., Wells G., Gazaryan I.G., Sakirolla R., Mohd Z, & Pichika M.R. (2022). Synthesis and Anti‐Inflammatory Activity of 2‐Amino‐4,5,6,7‐tetrahydrobenzo[b]thiophene‐Derived NRF2 Activators. ChemistryOpen, 11(10), e202200181.

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