Anti sr bi

The following primary mouse antibodies were used: anti-actin (Sigma-Aldrich, St. Louis, MO, United States, catalog No. A2228), anti-Flag (Sigma-Aldrich, St. Louis, MO, United States, catalog No. A2220), IgG control (MBL, Nagoya, Japan, catalog No. M075-3), anti-SR-BI (BD Biosciences, Franklin Lakes, NJ, United States, catalog No. 610883), and anti-UGGT1 (Santa Cruz Biotechnology, Santa Cruz, CA, United States, catalog No. Sc-374565). The following primary rabbit antibodies were used: anti-calnexin (Cell Signaling Technology, Boston, MA, United States, catalog No. 2433), anti-SR-BI (Novus Biological, Centennial, CO, United States, catalog No. NB400-104). The secondary antibodies included: HRP-conjugated ECL goat anti-rabbit IgG (Sigma-Aldrich, St. Louis, MO, United States, catalog No. A6154), HRP-conjugated ECL goat anti-mouse IgG (Sigma-Aldrich, St. Louis, MO, United States, catalog No. A4416), donkey anti-mouse-Alexa Fluor 488, donkey anti-rabbit-Alexa Fluor 594, donkey anti-rabbit-Alexa Fluor 488, donkey anti-mouse-Alexa Fluor 594, donkey anti-rat-Alexa Fluor 488 (Invitrogen, Carlsbad, CA, United States).

Proteins were isolated and equal amounts were separated by SDS-PAGE and transferred to nitrocellulose membranes (Sigma). After blocking, membranes were incubated with primary antibodies (anti-SR-BI, BD Biosciences 610882; anti-β-actin, Abcam ab8229; anti-CD36, Cayman 100011) at 4°C over-night. Membranes were then incubated with the appropriate horseradish peroxidase-coupled secondary antibodies followed by detection using the Super Signal chemiluminescence system (Thermo Scientific) and a Chemilmager 4440 (Biozym, Oldendorf, Germany).